Restriction map for
pF1KB7554
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BfaI BfrI BglII
BlnI BsaAI BsaI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I MaeI MaeII MaeIII
MfeI MflI MluI MroI MslI
MunI NcoI NdeI NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XhoII
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 463 AAATTC [GA]^AATT[CT]
AlwI 222 GATCC GGATC(4/5)
AlwNI 636 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 463 AAATTC [GA]^AATT[CT]
Asp700I 459 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 509 TGGCCA TGG^CCA
BbeI 31 GGCGCC GGCGC^C
BbsI 730 GAAGAC GAAGAC(2/6)
BglI 253 GCCGCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 525 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 525 GCTCAGC GC^T[ACGT]AGC
BpuAI 730 GAAGAC GAAGAC(2/6)
BsaBI 757 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 194 ACCGGA [AT]^CCGG[AT]
BsmBI 469 GAGACG CGTCTC(1/5)
BsrFI 21 GCCGGC [GA]^CCGG[CT]
BssSI 181 CTCGTG CACGAG(-5/-1)
BstXI 446 CCATCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 525 GCTCAGC GC^T[ACGT]AGC
Cfr10I 21 GCCGGC [GA]^CCGG[CT]
DdeI 526 CTCAG C^T[ACGT]AG
HaeII 31 GGCGCC [GA]GCGC^[CT]
KasI 31 GGCGCC G^GCGCC
MamI 757 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 509 TGGCCA TGG^CCA
MscI 509 TGGCCA TGG^CCA
MseI 800 TTAA T^TAA
NaeI 21 GCCGGC GCC^GGC
NarI 31 GGCGCC GG^CGCC
NgoMIV 21 GCCGGC G^CCGGC
PstI 520 CTGCAG CTGCA^G
SexAI 547 ACCTGGT A^CC[AT]GGT
SfaNI 516 GATGC GCATC(5/9)
SfcI 520 CTGCAG C^T[GA][CT]AG
SfoI 31 GGCGCC GGC^GCC
SmaI 16 CCCGGG CCC^GGG
StuI 394 AGGCCT AGG^CCT
Tru9I 800 TTAA T^TAA
Tsp509I 464 AATT ^AATT
TspRI 666 CAGTG CA[GC]TG[ACGT][ACGT]^
XmaCI 16 CCCGGG C^CCGGG
XmaI 16 CCCGGG C^CCGGG
XmnI 459 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 31 GGCGCC G[GA]^CG[CT]C
87 GACGCC
ApaI 121 GGGCCC GGGCC^C
291 GGGCCC
AvaI 16 CCCGGG C^[CT]CG[GA]G
493 CCCGAG
AvaII 197 GGACC G^G[AT]CC
274 GGACC
BanI 31 GGCGCC G^G[CT][GA]CC
42 GGTGCC
BclI 645 TGATCA T^GATCA
762 TGATCA
BpmI 375 CTGGAG CTGGAG(16/14)
454 CTGGAG
BsaHI 31 GGCGCC G[GA]^CG[CT]C
87 GACGCC
BseRI 165 CTCCTC GAGGAG(10/8)
576 GAGGAG
BsmFI 156 GTCCC GGGAC(10/14)
186 GTCCC
BsoBI 16 CCCGGG C^[CT]CG[GA]G
493 CCCGAG
BsrI 621 ACTGG ACTGG(1/-1)
665 CCAGT
BstAPI 333 GCACGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
654 GCAAGATTTGC
BstF5I 108 CATCC GGATG(2/0)
226 CATCC
DraII 290 GGGGCCC [GA]G^G[ACGT]CC[CT]
291 GGGCCCT
EcoO109I 290 GGGGCCC [GA]G^G[ACGT]CC[CT]
291 GGGCCCT
FbaI 645 TGATCA T^GATCA
762 TGATCA
FokI 108 CATCC GGATG(9/13)
226 CATCC
Hin1I 31 GGCGCC G[GA]^CG[CT]C
87 GACGCC
KspI 117 CCGCGG CCGC^GG
310 CCGCGG
SacII 117 CCGCGG CCGC^GG
310 CCGCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AfaI AluI BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BssKI BstNI
BstUI Cac8I CfoI Cfr13I DpnII
DsaI EaeI EcoRII FauI Fnu4HI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIV
RsaI Sau3AI Sau96I ScrFI TaqI
TfiI TseI TthHB8I