Restriction map for
pF1KB7714
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AvaI AvrII
BalI BamHI BbrPI BcgI BciVI
BfaI BfrI BglI BlnI BsaAI
BsiEI BsiWI BsmBI BsoBI BspDI
BsrDI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
HindIII HpaI KpnI KspI MaeI
MaeII MfeI MluI MluNI MscI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StyI SwaI
Tru9I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 361 GGCGCC G[GA]^CG[CT]C
AfaI 160 GTAC GT^AC
AhdI 172 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 172 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 361 GGCGCC GGCGC^C
BbvCI 339 GCTGAGG CCTCAGC(-5/-2)
BsaBI 561 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 361 GGCGCC G[GA]^CG[CT]C
BsmI 1249 GAATGC GAATGC(1/-1)
Bsp1407I 159 TGTACA T^GTACA
BspHI 281 TCATGA T^CATGA
BsrBI 429 GAGCGG CCGCTC(-3/-3)
BsrFI 1022 GCCGGC [GA]^CCGG[CT]
BsrGI 159 TGTACA T^GTACA
BstAPI 653 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1022 GCCGGC [GA]^CCGG[CT]
DraIII 1192 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 467 CCGTGG C^C[GA][CT]GG
EaeI 1020 TGGCCG [CT]^GGCC[GA]
Eam1105I 172 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1159 CTCTTC CTCTTC(1/4)
HaeII 361 GGCGCC [GA]GCGC^[CT]
HgaI 583 GACGC GACGC(5/10)
Hin1I 361 GGCGCC G[GA]^CG[CT]C
KasI 361 GGCGCC G^GCGCC
Ksp632I 1159 CTCTTC CTCTTC(1/4)
MaeIII 7 GTCAC ^GT[ACGT]AC
MamI 561 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1022 GCCGGC GCC^GGC
NarI 361 GGCGCC GG^CGCC
NgoMIV 1022 GCCGGC G^CCGGC
RcaI 281 TCATGA T^CATGA
RsaI 160 GTAC GT^AC
SacI 1312 GAGCTC GAGCT^C
SfoI 361 GGCGCC GGC^GCC
SspBI 159 TGTACA T^GTACA
StuI 401 AGGCCT AGG^CCT
TfiI 977 GATTC G^A[AT]TC
Tsp45I 7 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 996 CGCG CG^CG
1195 CGCG
AccIII 353 TCCGGA T^CCGGA
722 TCCGGA
AcsI 627 GAATTT [GA]^AATT[CT]
1090 AAATTC
ApoI 627 GAATTT [GA]^AATT[CT]
1090 AAATTC
AviII 413 TGCGCA TGC^GCA
1352 TGCGCA
BanI 344 GGTGCC G^G[CT][GA]CC
361 GGCGCC
BanII 1078 GAGCCC G[GA]GC[CT]^C
1312 GAGCTC
BbsI 381 GAAGAC GAAGAC(2/6)
1042 GAAGAC
BlpI 648 GCTTAGC GC^T[ACGT]AGC
1330 GCTCAGC
Bpu1102I 648 GCTTAGC GC^T[ACGT]AGC
1330 GCTCAGC
BpuAI 381 GAAGAC GAAGAC(2/6)
1042 GAAGAC
BsaI 126 GAGACC GGTCTC(1/5)
747 GGTCTC
BsaWI 353 TCCGGA [AT]^CCGG[AT]
722 TCCGGA
BseAI 353 TCCGGA T^CCGGA
722 TCCGGA
BsgI 61 CTGCAC GTGCAG(16/14)
535 GTGCAG
BspEI 353 TCCGGA T^CCGGA
722 TCCGGA
BspMI 48 ACCTGC ACCTGC(4/8)
1051 GCAGGT
BstUI 996 CGCG CG^CG
1195 CGCG
CelII 648 GCTTAGC GC^T[ACGT]AGC
1330 GCTCAGC
FspI 413 TGCGCA TGC^GCA
1352 TGCGCA
MroI 353 TCCGGA T^CCGGA
722 TCCGGA
MslI 271 CACTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1296 CATGGCTATG
MvnI 996 CGCG CG^CG
1195 CGCG
PpuMI 34 GGGTCCC [GA]G^G[AT]CC[CT]
225 AGGTCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AflIII AluI AlwNI AspHI
AvaII BbvI BclI BcnI BglII
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoNI EcoO109I EcoRII
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp509I TspRI TthHB8I
XhoII