Restriction map for
pF1KE0753
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwNI AocI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AviII AvrII BbeI
BbrPI BbsI BciVI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FauI FbaI
FseI FspI HapII HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MfeI MluI
MroI MspI MunI MvnI MwoI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TspRI Tth111I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccI 292 GTATAC GT^[AC][GT]AC
AcyI 706 GGCGTC G[GA]^CG[CT]C
AfaI 249 GTAC GT^AC
Alw44I 433 GTGCAC G^TGCAC
Aor51HI 344 AGCGCT AGC^GCT
ApaLI 433 GTGCAC G^TGCAC
AvaI 287 CTCGAG C^[CT]CG[GA]G
BalI 93 TGGCCA TGG^CCA
BamHI 338 GGATCC G^GATCC
BanI 546 GGTGCC G^G[CT][GA]CC
BanII 364 GAGCCC G[GA]GC[CT]^C
BbvCI 609 CCTCAGC CCTCAGC(-5/-2)
BcgI 422 GCAAGAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 724 CTAG C^TAG
BglII 653 AGATCT A^GATCT
BsaBI 187 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 706 GGCGTC G[GA]^CG[CT]C
BsgI 415 CTGCAC GTGCAG(16/14)
BsmAI 778 GAGAC GTCTC(1/5)
BsmFI 470 GGGAC GGGAC(10/14)
BsoBI 287 CTCGAG C^[CT]CG[GA]G
BspMI 413 ACCTGC ACCTGC(4/8)
BsrDI 405 CATTGC GCAATG(2/0)
BsrI 309 ACTGG ACTGG(1/-1)
Bst1107I 292 GTATAC GTA^TAC
BstXI 875 CCACCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 292 GTATAC GTA^TAC
CfoI 345 GCGC GCG^C
DraII 352 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 96 CCATGG C^C[GA][CT]GG
EaeI 93 TGGCCA [CT]^GGCC[GA]
Eco47III 344 AGCGCT AGC^GCT
EcoO109I 352 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 519 GATATC GAT^ATC
EcoT14I 96 CCATGG C^C[AT][AT]GG
HaeII 344 AGCGCT [GA]GCGC^[CT]
HgaI 707 GCGTC GACGC(5/10)
HhaI 345 GCGC GCG^C
Hin1I 706 GGCGTC G[GA]^CG[CT]C
HinP1I 345 GCGC G^CGC
HphI 322 GGTGA GGTGA(8/7)
MaeI 724 CTAG C^TAG
MaeIII 793 GTCAC ^GT[ACGT]AC
MamI 187 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 93 TGGCCA TGG^CCA
MscI 93 TGGCCA TGG^CCA
MslI 720 CACTCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 391 CAGCTG C[AC]G^C[GT]G
NcoI 96 CCATGG C^CATGG
PaeR7I 287 CTCGAG C^TCGAG
PflMI 558 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 780 GACTC GAGTC(4/5)
PvuII 391 CAGCTG CAG^CTG
RsaI 249 GTAC GT^AC
StuI 179 AGGCCT AGG^CCT
StyI 96 CCATGG C^C[AT][AT]GG
TaqI 288 TCGA T^CGA
TfiI 898 GATTC G^A[AT]TC
Tsp45I 793 GTCAC ^GT[GC]AC
Tsp509I 1005 AATT ^AATT
TthHB8I 288 TCGA T^CGA
Van91I 558 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 287 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 13 enzymes) #
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BpmI 844 CTCCAG CTGGAG(16/14)
696 CTGGAG
BstF5I 711 CATCC GGATG(2/0)
60 GGATG
BstYI 338 GGATCC [GA]^GATC[CT]
653 AGATCT
Cfr13I 269 GGGCC G^G[ACGT]CC
353 GGGCC
FokI 711 CATCC GGATG(9/13)
60 GGATG
HinfI 780 GACTC G^A[ACGT]TC
898 GATTC
MboII 174 GAAGA GAAGA(8/7)
516 GAAGA
MflI 338 GGATCC [GA]^GATC[CT]
653 AGATCT
MseI 197 TTAA T^TAA
475 TTAA
Sau96I 269 GGGCC G^G[ACGT]CC
353 GGGCC
Tru9I 197 TTAA T^TAA
475 TTAA
XcmI 96 CCATGGCTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
615 CCAGACTTACTGTGG
XhoII 338 GGATCC [GA]^GATC[CT]
653 AGATCT
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI AlwI AspHI BbvI
BmyI BsaJI BsiHKAI BsiYI BslI
Bsp1286I BssKI BstNI Cac8I DdeI
DpnII EcoRII Fnu4HI HaeIII ItaI
MboI MnlI MvaI NdeII NlaIII
NlaIV Sau3AI ScrFI SfaNI SmlI
TseI