Restriction map for
pF1KB7774
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwNI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I FauI FbaI Fnu4HI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI ItaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MspA1I MunI MvnI
MwoI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tru9I TseI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 575 GCGG CCGC(-3/-1)
Alw44I 1105 GTGCAC G^TGCAC
ApaLI 1105 GTGCAC G^TGCAC
AspHI 1105 GTGCAC G[AT]GC[AT]^C
BalI 209 TGGCCA TGG^CCA
BamHI 1121 GGATCC G^GATCC
BglII 61 AGATCT A^GATCT
BmyI 1105 GTGCAC G[AGT]GC[ACT]^C
BsaJI 282 CCATGG C^C[ACGT][ACGT]GG
BsaWI 1295 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1105 GTGCAC G[AT]GC[AT]^C
BsmFI 13 GGGAC GGGAC(10/14)
Bsp1286I 1105 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 1495 TGTACA T^GTACA
BspHI 492 TCATGA T^CATGA
BsrGI 1495 TGTACA T^GTACA
DraII 646 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 282 CCATGG C^C[GA][CT]GG
EaeI 209 TGGCCA [CT]^GGCC[GA]
EarI 247 GAAGAG CTCTTC(1/4)
EcoO109I 646 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 858 GATATC GAT^ATC
EcoT14I 282 CCATGG C^C[AT][AT]GG
EcoT22I 1219 ATGCAT ATGCA^T
HincII 1289 GTCAAC GT[CT]^[GA]AC
HindII 1289 GTCAAC GT[CT]^[GA]AC
Ksp632I 247 GAAGAG CTCTTC(1/4)
MaeII 453 ACGT A^CGT
MluNI 209 TGGCCA TGG^CCA
MscI 209 TGGCCA TGG^CCA
NcoI 282 CCATGG C^CATGG
NsiI 1219 ATGCAT ATGCA^T
PflMI 4 CCAGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1029 GACTC GAGTC(4/5)
RcaI 492 TCATGA T^CATGA
SapI 247 GAAGAGC GCTCTTC(1/4)
SspBI 1495 TGTACA T^GTACA
StyI 282 CCATGG C^C[AT][AT]GG
TaqI 86 TCGA T^CGA
Tsp45I 690 GTCAC ^GT[GC]AC
TthHB8I 86 TCGA T^CGA
Van91I 4 CCAGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AocI 275 CCTCAGG CC^T[ACGT]AGG
342 CCTCAGG
BpmI 169 CTGGAG CTGGAG(16/14)
722 CTGGAG
BsiYI 4 CCAGCTAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
201 CCTCTGCGTGG
BslI 4 CCAGCTAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
201 CCTCTGCGTGG
BsmI 571 GAATGC GAATGC(1/-1)
1221 GCATTC
BsrDI 615 CATTGC GCAATG(2/0)
317 GCAATG
BsrI 129 ACTGG ACTGG(1/-1)
721 ACTGG
Bsu36I 275 CCTCAGG CC^T[ACGT]AGG
342 CCTCAGG
Cac8I 3 GCCAGC GC[ACGT]^[ACGT]GC
1195 GCACGC
Eco81I 275 CCTCAGG CC^T[ACGT]AGG
342 CCTCAGG
HapII 1296 CCGG C^CGG
1440 CCGG
HindIII 506 AAGCTT A^AGCTT
1430 AAGCTT
HpaII 1296 CCGG C^CGG
1440 CCGG
MaeIII 287 GTTAC ^GT[ACGT]AC
690 GTCAC
MspI 1296 CCGG C^CGG
1440 CCGG
SfcI 177 CTACAG C^T[GA][CT]AG
513 CTGTAG
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AvaII BsaI BseRI BsmAI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII EcoRI EcoRII FokI
HaeIII HinfI HphI MboI MboII
MflI MnlI MslI MvaI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TfiI Tsp509I TspRI
XhoII XmnI