Restriction map for pF1KB7809
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
AocI        Aor51HI     AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       BspDI       BspEI       
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      Cac8I       CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HapII       HgaI        
Hin1I       HincII      HindII      HpaI        HpaII       
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


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# ONE CUT ENZYMES (   33 enzymes) #
###################################

AciI          217  GCGG                 CCGC(-3/-1)
AlwNI         358  CAGTTACTG            CAG[ACGT][ACGT][ACGT]^CTG
BalI          290  TGGCCA               TGG^CCA
BamHI        1061  GGATCC               G^GATCC
BbsI          152  GTCTTC               GAAGAC(2/6)
BglII        1326  AGATCT               A^GATCT
BpuAI         152  GTCTTC               GAAGAC(2/6)
BseRI          64  GAGGAG               GAGGAG(10/8)
BsmAI         310  GAGAC                GTCTC(1/5)
BspHI         756  TCATGA               T^CATGA
BspMI         246  ACCTGC               ACCTGC(4/8)
BsrDI         923  GCAATG               GCAATG(2/0)
BstAPI        244  GCACCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I         98  CATCC                GGATG(2/0)
CfoI          623  GCGC                 GCG^C
EaeI          290  TGGCCA               [CT]^GGCC[GA]
EcoNI         448  CCTGAGTTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI           98  CATCC                GGATG(9/13)
HaeII         622  GGCGCT               [GA]GCGC^[CT]
HhaI          623  GCGC                 GCG^C
HinP1I        623  GCGC                 G^CGC
MluNI         290  TGGCCA               TGG^CCA
MscI          290  TGGCCA               TGG^CCA
MwoI          244  GCACCTGCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI         1166  CATATG               CA^TATG
PpuMI         444  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI        1451  GACAGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          756  TCATGA               T^CATGA
SacI         1035  GAGCTC               GAGCT^C
SmlI          440  CTCAAG               C^T[CT][GA]AG
TaqI         1459  TCGA                 T^CGA
Tsp45I        545  GTCAC                ^GT[GC]AC
TthHB8I      1459  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AfaI          883  GTAC                 GT^AC
             1135  GTAC                
ApaI           42  GGGCCC               GGGCC^C
              137  GGGCCC              
AvaII         320  GGACC                G^G[AT]CC
              445  GGACC               
BanI          243  GGCACC               G^G[CT][GA]CC
              968  GGCACC              
BsaAI         666  TACGTG               [CT]AC^GT[GA]
             1336  TACGTG              
BsgI          145  GTGCAG               GTGCAG(16/14)
             1523  GTGCAG              
BsmFI         283  GGGAC                GGGAC(10/14)
              319  GGGAC               
Bsp1407I      882  TGTACA               T^GTACA
             1134  TGTACA              
BsrGI         882  TGTACA               T^GTACA
             1134  TGTACA              
BstYI        1061  GGATCC               [GA]^GATC[CT]
             1326  AGATCT              
DsaI           23  CCATGG               C^C[GA][CT]GG
               37  CCATGG              
EarI          178  GAAGAG               CTCTTC(1/4)
              568  GAAGAG              
HindIII       461  AAGCTT               A^AGCTT
              558  AAGCTT              
Ksp632I       178  GAAGAG               CTCTTC(1/4)
              568  GAAGAG              
MaeII         667  ACGT                 A^CGT
             1337  ACGT                
MflI         1061  GGATCC               [GA]^GATC[CT]
             1326  AGATCT              
NcoI           23  CCATGG               C^CATGG
               37  CCATGG              
PleI          285  GACTC                GAGTC(4/5)
              312  GACTC               
RsaI          883  GTAC                 GT^AC
             1135  GTAC                
SspBI         882  TGTACA               T^GTACA
             1134  TGTACA              
XhoII        1061  GGATCC               [GA]^GATC[CT]
             1326  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AcsI        AflIII      AluI        Alw44I      AlwI        
ApaLI       ApoI        Asp700I     AspHI       BanII       
BbvI        BfaI        BmyI        BpmI        BsaJI       
BsiHKAI     BsiYI       BslI        Bsp1286I    BspLU11I    
BsrI        BssKI       BstNI       Cfr13I      DdeI        
DpnII       DraII       EcoO109I    EcoRI       EcoRII      
EcoT14I     Fnu4HI      HaeIII      HinfI       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MnlI        MseI        MslI        MvaI        NdeII       
NlaIII      NlaIV       NspI        PstI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StuI        
StyI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       XmnI        
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