################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AciI 217 GCGG CCGC(-3/-1) AlwNI 358 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG BalI 290 TGGCCA TGG^CCA BamHI 1061 GGATCC G^GATCC BbsI 152 GTCTTC GAAGAC(2/6) BglII 1326 AGATCT A^GATCT BpuAI 152 GTCTTC GAAGAC(2/6) BseRI 64 GAGGAG GAGGAG(10/8) BsmAI 310 GAGAC GTCTC(1/5) BspHI 756 TCATGA T^CATGA BspMI 246 ACCTGC ACCTGC(4/8) BsrDI 923 GCAATG GCAATG(2/0) BstAPI 244 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 98 CATCC GGATG(2/0) CfoI 623 GCGC GCG^C EaeI 290 TGGCCA [CT]^GGCC[GA] EcoNI 448 CCTGAGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 98 CATCC GGATG(9/13) HaeII 622 GGCGCT [GA]GCGC^[CT] HhaI 623 GCGC GCG^C HinP1I 623 GCGC G^CGC MluNI 290 TGGCCA TGG^CCA MscI 290 TGGCCA TGG^CCA MwoI 244 GCACCTGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 1166 CATATG CA^TATG PpuMI 444 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1451 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 756 TCATGA T^CATGA SacI 1035 GAGCTC GAGCT^C SmlI 440 CTCAAG C^T[CT][GA]AG TaqI 1459 TCGA T^CGA Tsp45I 545 GTCAC ^GT[GC]AC TthHB8I 1459 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 883 GTAC GT^AC 1135 GTAC ApaI 42 GGGCCC GGGCC^C 137 GGGCCC AvaII 320 GGACC G^G[AT]CC 445 GGACC BanI 243 GGCACC G^G[CT][GA]CC 968 GGCACC BsaAI 666 TACGTG [CT]AC^GT[GA] 1336 TACGTG BsgI 145 GTGCAG GTGCAG(16/14) 1523 GTGCAG BsmFI 283 GGGAC GGGAC(10/14) 319 GGGAC Bsp1407I 882 TGTACA T^GTACA 1134 TGTACA BsrGI 882 TGTACA T^GTACA 1134 TGTACA BstYI 1061 GGATCC [GA]^GATC[CT] 1326 AGATCT DsaI 23 CCATGG C^C[GA][CT]GG 37 CCATGG EarI 178 GAAGAG CTCTTC(1/4) 568 GAAGAG HindIII 461 AAGCTT A^AGCTT 558 AAGCTT Ksp632I 178 GAAGAG CTCTTC(1/4) 568 GAAGAG MaeII 667 ACGT A^CGT 1337 ACGT MflI 1061 GGATCC [GA]^GATC[CT] 1326 AGATCT NcoI 23 CCATGG C^CATGG 37 CCATGG PleI 285 GACTC GAGTC(4/5) 312 GACTC RsaI 883 GTAC GT^AC 1135 GTAC SspBI 882 TGTACA T^GTACA 1134 TGTACA XhoII 1061 GGATCC [GA]^GATC[CT] 1326 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AcsI AflIII AluI Alw44I AlwI ApaLI ApoI Asp700I AspHI BanII BbvI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI Bsp1286I BspLU11I BsrI BssKI BstNI Cfr13I DdeI DpnII DraII EcoO109I EcoRI EcoRII EcoT14I Fnu4HI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MvaI NdeII NlaIII NlaIV NspI PstI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TfiI Tru9I TseI Tsp509I TspRI XmnI