Restriction map for
pF1KB7854
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI AlwI
AlwNI AocI Aor51HI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaHI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
FseI FspI Hin1I HincII HindII
HpaI KasI KpnI KspI MfeI
MflI MluI MspA1I MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XcmI
XhoII
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccIII 633 TCCGGA T^CCGGA
AflIII 713 ACATGT A^C[GA][CT]GT
Alw44I 514 GTGCAC G^TGCAC
ApaI 792 GGGCCC GGGCC^C
ApaLI 514 GTGCAC G^TGCAC
AseI 160 ATTAAT AT^TAAT
AsnI 160 ATTAAT AT^TAAT
Asp700I 1095 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 590 TGGCCA TGG^CCA
BanI 295 GGCACC G^G[CT][GA]CC
BbvCI 703 GCTGAGG CCTCAGC(-5/-2)
BcgI 432 CGAGAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 720 TGATCA T^GATCA
BfaI 31 CTAG C^TAG
BglI 139 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 391 CTGGAG CTGGAG(16/14)
BsaAI 1621 TACGTG [CT]AC^GT[GA]
BsaBI 1098 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 844 GAGACC GGTCTC(1/5)
BseAI 633 TCCGGA T^CCGGA
BspEI 633 TCCGGA T^CCGGA
BspLU11I 713 ACATGT A^CATGT
BspMI 1466 ACCTGC ACCTGC(4/8)
BsrBI 1285 GAGCGG CCGCTC(-3/-3)
BsrDI 999 CATTGC GCAATG(2/0)
BstAPI 1051 GCATATAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EaeI 590 TGGCCA [CT]^GGCC[GA]
EcoRI 396 GAATTC G^AATTC
EcoRV 1698 GATATC GAT^ATC
EcoT22I 1652 ATGCAT ATGCA^T
FauI 405 GCGGG CCCGC(4/6)
FbaI 720 TGATCA T^GATCA
HaeII 601 AGCGCC [GA]GCGC^[CT]
HgaI 664 GACGC GACGC(5/10)
HindIII 1738 AAGCTT A^AGCTT
MaeI 31 CTAG C^TAG
MamI 1098 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 590 TGGCCA TGG^CCA
MroI 633 TCCGGA T^CCGGA
MscI 590 TGGCCA TGG^CCA
NcoI 1550 CCATGG C^CATGG
NsiI 1652 ATGCAT ATGCA^T
NspI 713 ACATGT [GA]CATG^[CT]
PaeR7I 221 CTCGAG C^TCGAG
PpuMI 989 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 160 ATTAAT AT^TAAT
PstI 1468 CTGCAG CTGCA^G
SacI 435 GAGCTC GAGCT^C
SfcI 1468 CTGCAG C^T[GA][CT]AG
SmaI 524 CCCGGG CCC^GGG
SpeI 30 ACTAGT A^CTAGT
StuI 1319 AGGCCT AGG^CCT
XhoI 221 CTCGAG C^TCGAG
XmaCI 524 CCCGGG C^CCGGG
XmaI 524 CCCGGG C^CCGGG
XmnI 1095 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AciI 405 GCGG CCGC(-3/-1)
1287 GCGG
AcsI 396 GAATTC [GA]^AATT[CT]
679 AAATTT
ApoI 396 GAATTC [GA]^AATT[CT]
679 AAATTT
BanII 435 GAGCTC G[GA]GC[CT]^C
792 GGGCCC
BsmFI 1076 GGGAC GGGAC(10/14)
1600 GGGAC
Cac8I 194 GCCTGC GC[ACGT]^[ACGT]GC
588 GCTGGC
CfoI 602 GCGC GCG^C
624 GCGC
DsaI 1550 CCATGG C^C[GA][CT]GG
1721 CCGTGG
HhaI 602 GCGC GCG^C
624 GCGC
HinP1I 602 GCGC G^CGC
624 GCGC
PleI 567 GACTC GAGTC(4/5)
10 GAGTC
SmlI 221 CTCGAG C^T[CT][GA]AG
1119 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AfaI AluI AspHI AvaI AvaII
BbsI BbvI BcnI BmyI BpuAI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I