Restriction map for
pF1KE0773
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BbeI BbrPI
BbvCI BciVI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KasI KpnI MluI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
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AccIII 86 TCCGGA T^CCGGA
AcyI 239 GGCGTC G[GA]^CG[CT]C
AflIII 269 ACGTGT A^C[GA][CT]GT
AocI 681 CCTGAGG CC^T[ACGT]AGG
AvaI 281 CTCGGG C^[CT]CG[GA]G
AviII 365 TGCGCA TGC^GCA
BalI 159 TGGCCA TGG^CCA
BanI 703 GGCACC G^G[CT][GA]CC
BbsI 690 GAAGAC GAAGAC(2/6)
BcgI 368 GCAGAGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 981 CCGGG CC^[GC]GG
BfaI 460 CTAG C^TAG
BpuAI 690 GAAGAC GAAGAC(2/6)
BsaBI 375 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 239 GGCGTC G[GA]^CG[CT]C
BseAI 86 TCCGGA T^CCGGA
BsiEI 36 CGGCCG CG[GA][CT]^CG
BsmBI 415 CGTCTC CGTCTC(1/5)
BsoBI 281 CTCGGG C^[CT]CG[GA]G
BspEI 86 TCCGGA T^CCGGA
BspHI 122 TCATGA T^CATGA
BsrBI 129 CCGCTC CCGCTC(-3/-3)
BstAPI 357 GCATCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 533 CCACGATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 681 CCTGAGG CC^T[ACGT]AGG
EagI 36 CGGCCG C^GGCCG
EclXI 36 CGGCCG C^GGCCG
Eco52I 36 CGGCCG C^GGCCG
Eco81I 681 CCTGAGG CC^T[ACGT]AGG
FspI 365 TGCGCA TGC^GCA
HaeII 309 GGCGCT [GA]GCGC^[CT]
Hin1I 239 GGCGTC G[GA]^CG[CT]C
KspI 474 CCGCGG CCGC^GG
MaeI 460 CTAG C^TAG
MaeII 269 ACGT A^CGT
MaeIII 100 GTCAC ^GT[ACGT]AC
MamI 375 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 218 CAATTG C^AATTG
MluNI 159 TGGCCA TGG^CCA
MroI 86 TCCGGA T^CCGGA
MscI 159 TGGCCA TGG^CCA
MseI 202 TTAA T^TAA
MunI 218 CAATTG C^AATTG
NciI 981 CCGGG CC^[GC]GG
NcoI 573 CCATGG C^CATGG
PleI 80 GAGTC GAGTC(4/5)
PstI 496 CTGCAG CTGCA^G
RcaI 122 TCATGA T^CATGA
SacI 481 GAGCTC GAGCT^C
SacII 474 CCGCGG CCGC^GG
SapI 501 GAAGAGC GCTCTTC(1/4)
SfcI 496 CTGCAG C^T[GA][CT]AG
TaqI 377 TCGA T^CGA
Tru9I 202 TTAA T^TAA
Tsp45I 100 GTCAC ^GT[GC]AC
Tsp509I 219 AATT ^AATT
TspRI 183 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 377 TCGA T^CGA
XbaI 459 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 206 CGCG CG^CG
475 CGCG
AfaI 273 GTAC GT^AC
567 GTAC
AlwI 446 GGATC GGATC(4/5)
762 GGATC
AspHI 63 GAGCAC G[AT]GC[AT]^C
481 GAGCTC
BsaI 838 GAGACC GGTCTC(1/5)
960 GGTCTC
BsaWI 86 TCCGGA [AT]^CCGG[AT]
437 ACCGGA
BsiHKAI 63 GAGCAC G[AT]GC[AT]^C
481 GAGCTC
BsmFI 187 GGGAC GGGAC(10/14)
330 GTCCC
BspMI 392 ACCTGC ACCTGC(4/8)
716 ACCTGC
BstF5I 400 CATCC GGATG(2/0)
408 CATCC
BstUI 206 CGCG CG^CG
475 CGCG
BstYI 446 GGATCT [GA]^GATC[CT]
762 GGATCT
Cac8I 669 GCATGC GC[ACGT]^[ACGT]GC
743 GCATGC
DraII 317 AGGTCCT [GA]G^G[ACGT]CC[CT]
902 AGGACCT
EaeI 36 CGGCCG [CT]^GGCC[GA]
159 TGGCCA
EarI 322 CTCTTC CTCTTC(1/4)
501 GAAGAG
EcoNI 677 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
951 CCTGGCCCAGG
EcoO109I 317 AGGTCCT [GA]G^G[ACGT]CC[CT]
902 AGGACCT
EcoT14I 573 CCATGG C^C[AT][AT]GG
973 CCTTGG
FokI 400 CATCC GGATG(9/13)
408 CATCC
HgaI 240 GCGTC GACGC(5/10)
414 GCGTC
Ksp632I 322 CTCTTC CTCTTC(1/4)
501 GAAGAG
MflI 446 GGATCT [GA]^GATC[CT]
762 GGATCT
MslI 534 CACGATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
774 CATTGGAGTG
MvnI 206 CGCG CG^CG
475 CGCG
MwoI 357 GCATCAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
618 GCTCTTAGGGC
NspI 669 GCATGC [GA]CATG^[CT]
743 GCATGC
PpuMI 317 AGGTCCT [GA]G^G[AT]CC[CT]
902 AGGACCT
PvuII 149 CAGCTG CAG^CTG
361 CAGCTG
RsaI 273 GTAC GT^AC
567 GTAC
SmlI 313 CTCAAG C^T[CT][GA]AG
628 CTCAAG
SphI 669 GCATGC GCATG^C
743 GCATGC
StyI 573 CCATGG C^C[AT][AT]GG
973 CCTTGG
TfiI 456 GATTC G^A[AT]TC
588 GATTC
XhoII 446 GGATCT [GA]^GATC[CT]
762 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BmyI BsaJI BseRI BsgI BsiYI
BslI BsmAI Bsp1286I BssKI BstNI
CfoI Cfr13I DdeI DpnII DsaI
EcoRII FauI Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MboI MboII MnlI MspA1I
MspI MvaI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TseI