Restriction map for
pF1KE0644
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspI AvaI AvrII
BalI BamHI BanII BbeI BbsI
BbvCI BciVI BclI BfrI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiWI
BsmAI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BssHII Bst1107I BstAPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRV FbaI
FseI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspI PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AhdI 95 GACCGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 625 GTGCAC G^TGCAC
AlwI 529 GGATC GGATC(4/5)
ApaLI 625 GTGCAC G^TGCAC
AspEI 95 GACCGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 227 TGCGCA TGC^GCA
BbrPI 544 CACGTG CAC^GTG
BcgI 27 GCAGTTCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 496 CTAG C^TAG
BglI 130 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 635 AGATCT A^GATCT
BseRI 1213 GAGGAG GAGGAG(10/8)
BsiEI 94 CGACCG CG[GA][CT]^CG
Bsp1407I 698 TGTACA T^GTACA
BsrBI 97 CCGCTC CCGCTC(-3/-3)
BsrGI 698 TGTACA T^GTACA
BssSI 142 CTCGTG CACGAG(-5/-1)
BstBI 758 TTCGAA TT^CGAA
BstEII 207 GGTGACC G^GT[ACGT]ACC
BstPI 207 GGTGACC G^GT[ACGT]ACC
BstYI 635 AGATCT [GA]^GATC[CT]
DraI 451 TTTAAA TTT^AAA
Eam1105I 95 GACCGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 207 GGTGACC G^GT[ACGT]ACC
EcoRI 1269 GAATTC G^AATTC
EcoT14I 257 CCTTGG C^C[AT][AT]GG
EcoT22I 995 ATGCAT ATGCA^T
FspI 227 TGCGCA TGC^GCA
HinfI 1140 GAATC G^A[ACGT]TC
MaeI 496 CTAG C^TAG
MflI 635 AGATCT [GA]^GATC[CT]
MspA1I 380 CCGCTG C[AC]G^C[GT]G
NdeI 877 CATATG CA^TATG
NsiI 995 ATGCAT ATGCA^T
NspV 758 TTCGAA TT^CGAA
PmaCI 544 CACGTG CAC^GTG
PmlI 544 CACGTG CAC^GTG
PstI 1034 CTGCAG CTGCA^G
SfcI 1034 CTGCAG C^T[GA][CT]AG
SfuI 758 TTCGAA TT^CGAA
SgrAI 150 CACCGGCG C[GA]^CCGG[CT]G
SmlI 556 CTTGAG C^T[CT][GA]AG
SspBI 698 TGTACA T^GTACA
StyI 257 CCTTGG C^C[AT][AT]GG
TaqI 759 TCGA T^CGA
TfiI 1140 GAATC G^A[AT]TC
TthHB8I 759 TCGA T^CGA
XhoII 635 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 103 GTCTAC GT^[AC][GT]AC
375 GTCTAC
AccII 62 CGCG CG^CG
127 CGCG
AcsI 1089 AAATTC [GA]^AATT[CT]
1269 GAATTC
AfaI 84 GTAC GT^AC
699 GTAC
ApoI 1089 AAATTC [GA]^AATT[CT]
1269 GAATTC
AvaII 48 GGACC G^G[AT]CC
614 GGACC
BcnI 21 CCCGG CC^[GC]GG
121 CCGGG
BpmI 310 CTCCAG CTGGAG(16/14)
1070 CTCCAG
BsaAI 202 TACGTG [CT]AC^GT[GA]
544 CACGTG
BsmI 293 GCATTC GAATGC(1/-1)
1168 GCATTC
BspMI 272 ACCTGC ACCTGC(4/8)
409 ACCTGC
BsrDI 414 CATTGC GCAATG(2/0)
185 GCAATG
BsrFI 86 ACCGGC [GA]^CCGG[CT]
151 ACCGGC
BstUI 62 CGCG CG^CG
127 CGCG
Cfr10I 86 ACCGGC [GA]^CCGG[CT]
151 ACCGGC
EarI 664 CTCTTC CTCTTC(1/4)
607 GAAGAG
HaeIII 138 GGCC GG^CC
222 GGCC
HgaI 60 GACGC GACGC(5/10)
125 GACGC
Ksp632I 664 CTCTTC CTCTTC(1/4)
607 GAAGAG
MaeII 203 ACGT A^CGT
545 ACGT
MseI 11 TTAA T^TAA
452 TTAA
MvnI 62 CGCG CG^CG
127 CGCG
NciI 21 CCCGG CC^[GC]GG
121 CCGGG
RsaI 84 GTAC GT^AC
699 GTAC
Tru9I 11 TTAA T^TAA
452 TTAA
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AspHI BanI BbvI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII FauI Fnu4HI FokI
HaeII HapII HhaI HinP1I HpaII
HphI ItaI MaeIII MboI MboII
MnlI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I Tsp509I
TspRI