Restriction map for pF1KB6952
##################################
# NO CUT ENZYMES (  177 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AvaI        
AviII       AvrII       BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaJI       BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmI        BsoBI       BspDI       
BspEI       BspLU11I    BsrFI       BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRII      
EcoRV       EcoT14I     EcoT22I     FbaI        FokI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HpaII       
KasI        KpnI        KspI        MaeIII      MamI        
MfeI        MluI        MroI        MspA1I      MspI        
MunI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        ScrFI       SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    StuI        
StyI        SwaI        TaqI        Tsp45I      TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AciI          183  CCGC                 CCGC(-3/-1)
AfaI          679  GTAC                 GT^AC
AflIII        568  ACACGT               A^C[GA][CT]GT
AspI          140  GACTAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         379  GGACC                G^G[AT]CC
BalI          206  TGGCCA               TGG^CCA
BbvI          523  GCTGC                GCAGC(8/12)
BfaI          289  CTAG                 C^TAG
BglII         193  AGATCT               A^GATCT
BmyI          199  GGGCAC               G[AGT]GC[ACT]^C
BpmI          186  CTCCAG               CTGGAG(16/14)
BsaI          621  GAGACC               GGTCTC(1/5)
BsmBI         639  GAGACG               CGTCTC(1/5)
BsmFI         378  GGGAC                GGGAC(10/14)
Bsp1286I      199  GGGCAC               G[AGT]GC[ACT]^C
Bsp1407I      678  TGTACA               T^GTACA
BspHI         171  TCATGA               T^CATGA
BspMI         658  ACCTGC               ACCTGC(4/8)
BsrBI         183  CCGCTC               CCGCTC(-3/-3)
BsrGI         678  TGTACA               T^GTACA
BsrI          188  CCAGT                ACTGG(1/-1)
BstXI         276  CCATAGAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         193  AGATCT               [GA]^GATC[CT]
Cfr13I        379  GGACC                G^G[ACGT]CC
DraII         378  GGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI          206  TGGCCA               [CT]^GGCC[GA]
EarI          232  GAAGAG               CTCTTC(1/4)
EcoO109I      378  GGGACCT              [GA]G^G[ACGT]CC[CT]
FauI          182  CCCGC                CCCGC(4/6)
Fnu4HI        523  GCTGC                GC^[ACGT]GC
HaeIII        207  GGCC                 GG^CC
HincII        451  GTTAAC               GT[CT]^[GA]AC
HindII        451  GTTAAC               GT[CT]^[GA]AC
HpaI          451  GTTAAC               GTT^AAC
ItaI          523  GCTGC                GC^[ACGT]GC
Ksp632I       232  GAAGAG               CTCTTC(1/4)
MaeI          289  CTAG                 C^TAG
MaeII         570  ACGT                 A^CGT
MflI          193  AGATCT               [GA]^GATC[CT]
MluNI         206  TGGCCA               TGG^CCA
MscI          206  TGGCCA               TGG^CCA
MslI          277  CATAGAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIV         378  GGGACC               GG[ACGT]^[ACGT]CC
PflFI         140  GACTAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          221  GAGTC                GAGTC(4/5)
PpuMI         378  GGGACCT              [GA]G^G[AT]CC[CT]
RcaI          171  TCATGA               T^CATGA
RsaI          679  GTAC                 GT^AC
Sau96I        379  GGACC                G^G[ACGT]CC
SspBI         678  TGTACA               T^GTACA
SspI          334  AATATT               AAT^ATT
TseI          523  GCTGC                G^C[AT]GC
Tth111I       140  GACTAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         193  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AluI          120  AGCT                 AG^CT
              545  AGCT                
BsgI          524  CTGCAC               GTGCAG(16/14)
              154  GTGCAG              
BsiYI         228  CCCTGAAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              441  CCCAAAAGAGG         
BslI          228  CCCTGAAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              441  CCCAAAAGAGG         
BsrDI          11  CATTGC               GCAATG(2/0)
              662  GCAATG              
DpnII         194  GATC                 ^GATC
              644  GATC                
EcoRI         319  GAATTC               G^AATTC
              667  GAATTC              
HphI          250  GGTGA                GGTGA(8/7)
              393  TCACC               
MboI          194  GATC                 ^GATC
              644  GATC                
NdeII         194  GATC                 ^GATC
              644  GATC                
Sau3AI        194  GATC                 ^GATC
              644  GATC                
TfiI           43  GAATC                G^A[AT]TC
              253  GAATC               
TspRI         239  CAGTG                CA[GC]TG[ACGT][ACGT]^
              324  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AcsI        ApoI        BbsI        BpuAI       BsmAI       
DdeI        HinfI       MboII       MnlI        MseI        
NlaIII      SfaNI       Tru9I       Tsp509I     
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