################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaJI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmI BsoBI BspDI BspEI BspLU11I BsrFI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRII EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaII KasI KpnI KspI MaeIII MamI MfeI MluI MroI MspA1I MspI MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI StyI SwaI TaqI Tsp45I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AciI 183 CCGC CCGC(-3/-1) AfaI 679 GTAC GT^AC AflIII 568 ACACGT A^C[GA][CT]GT AspI 140 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 379 GGACC G^G[AT]CC BalI 206 TGGCCA TGG^CCA BbvI 523 GCTGC GCAGC(8/12) BfaI 289 CTAG C^TAG BglII 193 AGATCT A^GATCT BmyI 199 GGGCAC G[AGT]GC[ACT]^C BpmI 186 CTCCAG CTGGAG(16/14) BsaI 621 GAGACC GGTCTC(1/5) BsmBI 639 GAGACG CGTCTC(1/5) BsmFI 378 GGGAC GGGAC(10/14) Bsp1286I 199 GGGCAC G[AGT]GC[ACT]^C Bsp1407I 678 TGTACA T^GTACA BspHI 171 TCATGA T^CATGA BspMI 658 ACCTGC ACCTGC(4/8) BsrBI 183 CCGCTC CCGCTC(-3/-3) BsrGI 678 TGTACA T^GTACA BsrI 188 CCAGT ACTGG(1/-1) BstXI 276 CCATAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 193 AGATCT [GA]^GATC[CT] Cfr13I 379 GGACC G^G[ACGT]CC DraII 378 GGGACCT [GA]G^G[ACGT]CC[CT] EaeI 206 TGGCCA [CT]^GGCC[GA] EarI 232 GAAGAG CTCTTC(1/4) EcoO109I 378 GGGACCT [GA]G^G[ACGT]CC[CT] FauI 182 CCCGC CCCGC(4/6) Fnu4HI 523 GCTGC GC^[ACGT]GC HaeIII 207 GGCC GG^CC HincII 451 GTTAAC GT[CT]^[GA]AC HindII 451 GTTAAC GT[CT]^[GA]AC HpaI 451 GTTAAC GTT^AAC ItaI 523 GCTGC GC^[ACGT]GC Ksp632I 232 GAAGAG CTCTTC(1/4) MaeI 289 CTAG C^TAG MaeII 570 ACGT A^CGT MflI 193 AGATCT [GA]^GATC[CT] MluNI 206 TGGCCA TGG^CCA MscI 206 TGGCCA TGG^CCA MslI 277 CATAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIV 378 GGGACC GG[ACGT]^[ACGT]CC PflFI 140 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 221 GAGTC GAGTC(4/5) PpuMI 378 GGGACCT [GA]G^G[AT]CC[CT] RcaI 171 TCATGA T^CATGA RsaI 679 GTAC GT^AC Sau96I 379 GGACC G^G[ACGT]CC SspBI 678 TGTACA T^GTACA SspI 334 AATATT AAT^ATT TseI 523 GCTGC G^C[AT]GC Tth111I 140 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 193 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AluI 120 AGCT AG^CT 545 AGCT BsgI 524 CTGCAC GTGCAG(16/14) 154 GTGCAG BsiYI 228 CCCTGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 441 CCCAAAAGAGG BslI 228 CCCTGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 441 CCCAAAAGAGG BsrDI 11 CATTGC GCAATG(2/0) 662 GCAATG DpnII 194 GATC ^GATC 644 GATC EcoRI 319 GAATTC G^AATTC 667 GAATTC HphI 250 GGTGA GGTGA(8/7) 393 TCACC MboI 194 GATC ^GATC 644 GATC NdeII 194 GATC ^GATC 644 GATC Sau3AI 194 GATC ^GATC 644 GATC TfiI 43 GAATC G^A[AT]TC 253 GAATC TspRI 239 CAGTG CA[GC]TG[ACGT][ACGT]^ 324 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AcsI ApoI BbsI BpuAI BsmAI DdeI HinfI MboII MnlI MseI NlaIII SfaNI Tru9I Tsp509I