Restriction map for
pF1KB6952
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaJI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmI BsoBI BspDI
BspEI BspLU11I BsrFI BssHII BssKI
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstNI BstPI BstUI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRII
EcoRV EcoT14I EcoT22I FbaI FokI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaII
KasI KpnI KspI MaeIII MamI
MfeI MluI MroI MspA1I MspI
MunI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI ScrFI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
StyI SwaI TaqI Tsp45I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AciI 183 CCGC CCGC(-3/-1)
AfaI 679 GTAC GT^AC
AflIII 568 ACACGT A^C[GA][CT]GT
AspI 140 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 379 GGACC G^G[AT]CC
BalI 206 TGGCCA TGG^CCA
BbvI 523 GCTGC GCAGC(8/12)
BfaI 289 CTAG C^TAG
BglII 193 AGATCT A^GATCT
BmyI 199 GGGCAC G[AGT]GC[ACT]^C
BpmI 186 CTCCAG CTGGAG(16/14)
BsaI 621 GAGACC GGTCTC(1/5)
BsmBI 639 GAGACG CGTCTC(1/5)
BsmFI 378 GGGAC GGGAC(10/14)
Bsp1286I 199 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 678 TGTACA T^GTACA
BspHI 171 TCATGA T^CATGA
BspMI 658 ACCTGC ACCTGC(4/8)
BsrBI 183 CCGCTC CCGCTC(-3/-3)
BsrGI 678 TGTACA T^GTACA
BsrI 188 CCAGT ACTGG(1/-1)
BstXI 276 CCATAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 193 AGATCT [GA]^GATC[CT]
Cfr13I 379 GGACC G^G[ACGT]CC
DraII 378 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 206 TGGCCA [CT]^GGCC[GA]
EarI 232 GAAGAG CTCTTC(1/4)
EcoO109I 378 GGGACCT [GA]G^G[ACGT]CC[CT]
FauI 182 CCCGC CCCGC(4/6)
Fnu4HI 523 GCTGC GC^[ACGT]GC
HaeIII 207 GGCC GG^CC
HincII 451 GTTAAC GT[CT]^[GA]AC
HindII 451 GTTAAC GT[CT]^[GA]AC
HpaI 451 GTTAAC GTT^AAC
ItaI 523 GCTGC GC^[ACGT]GC
Ksp632I 232 GAAGAG CTCTTC(1/4)
MaeI 289 CTAG C^TAG
MaeII 570 ACGT A^CGT
MflI 193 AGATCT [GA]^GATC[CT]
MluNI 206 TGGCCA TGG^CCA
MscI 206 TGGCCA TGG^CCA
MslI 277 CATAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIV 378 GGGACC GG[ACGT]^[ACGT]CC
PflFI 140 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 221 GAGTC GAGTC(4/5)
PpuMI 378 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 171 TCATGA T^CATGA
RsaI 679 GTAC GT^AC
Sau96I 379 GGACC G^G[ACGT]CC
SspBI 678 TGTACA T^GTACA
SspI 334 AATATT AAT^ATT
TseI 523 GCTGC G^C[AT]GC
Tth111I 140 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 193 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AluI 120 AGCT AG^CT
545 AGCT
BsgI 524 CTGCAC GTGCAG(16/14)
154 GTGCAG
BsiYI 228 CCCTGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
441 CCCAAAAGAGG
BslI 228 CCCTGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
441 CCCAAAAGAGG
BsrDI 11 CATTGC GCAATG(2/0)
662 GCAATG
DpnII 194 GATC ^GATC
644 GATC
EcoRI 319 GAATTC G^AATTC
667 GAATTC
HphI 250 GGTGA GGTGA(8/7)
393 TCACC
MboI 194 GATC ^GATC
644 GATC
NdeII 194 GATC ^GATC
644 GATC
Sau3AI 194 GATC ^GATC
644 GATC
TfiI 43 GAATC G^A[AT]TC
253 GAATC
TspRI 239 CAGTG CA[GC]TG[ACGT][ACGT]^
324 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AcsI ApoI BbsI BpuAI BsmAI
DdeI HinfI MboII MnlI MseI
NlaIII SfaNI Tru9I Tsp509I