################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## Acc65I AccI AccII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanII BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfaI BfrI BglI BlnI BpuAI BsaBI BsaI BseRI BsgI BsiEI BsiWI BsmI BspDI BspHI BspLU11I BspMI BsrDI BsrFI BssHII BssKI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MspA1I MunI MvaI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 502 GACGTC GACGT^C AccIII 47 TCCGGA T^CCGGA AcsI 130 AAATTT [GA]^AATT[CT] Aor51HI 704 AGCGCT AGC^GCT ApoI 130 AAATTT [GA]^AATT[CT] AvaII 112 GGTCC G^G[AT]CC BamHI 44 GGATCC G^GATCC BanI 780 GGTGCC G^G[CT][GA]CC BcgI 53 GCAGACGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaWI 47 TCCGGA [AT]^CCGG[AT] BseAI 47 TCCGGA T^CCGGA BsmBI 641 GAGACG CGTCTC(1/5) Bsp1407I 450 TGTACA T^GTACA BspEI 47 TCCGGA T^CCGGA BsrBI 754 GAGCGG CCGCTC(-3/-3) BsrGI 450 TGTACA T^GTACA BssSI 700 CACGAG CACGAG(-5/-1) BstF5I 599 GGATG GGATG(2/0) DsaI 776 CCATGG C^C[GA][CT]GG Eco47III 704 AGCGCT AGC^GCT FauI 756 GCGGG CCCGC(4/6) FokI 599 GGATG GGATG(9/13) HapII 48 CCGG C^CGG HgaI 56 GACGC GACGC(5/10) HpaII 48 CCGG C^CGG HphI 613 GGTGA GGTGA(8/7) MaeIII 175 GTTAC ^GT[ACGT]AC MroI 47 TCCGGA T^CCGGA MseI 14 TTAA T^TAA MslI 774 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 48 CCGG C^CGG NcoI 776 CCATGG C^CATGG NdeI 479 CATATG CA^TATG NspI 231 ACATGC [GA]CATG^[CT] PleI 518 GAGTC GAGTC(4/5) ScaI 315 AGTACT AGT^ACT SmlI 292 CTTGAG C^T[CT][GA]AG SspBI 450 TGTACA T^GTACA Tru9I 14 TTAA T^TAA TspRI 384 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 56 GACGCC G[GA]^CG[CT]C 502 GACGTC AspHI 771 GAGCAC G[AT]GC[AT]^C 913 GAGCAC AvaI 61 CTCGGG C^[CT]CG[GA]G 638 CCCGAG BalI 380 TGGCCA TGG^CCA 683 TGGCCA BglII 288 AGATCT A^GATCT 907 AGATCT BlpI 540 GCTAAGC GC^T[ACGT]AGC 768 GCTGAGC Bpu1102I 540 GCTAAGC GC^T[ACGT]AGC 768 GCTGAGC BsaAI 37 TACGTG [CT]AC^GT[GA] 573 TACGTG BsaHI 56 GACGCC G[GA]^CG[CT]C 502 GACGTC BsiHKAI 771 GAGCAC G[AT]GC[AT]^C 913 GAGCAC BsmFI 500 GGGAC GGGAC(10/14) 834 GGGAC BsoBI 61 CTCGGG C^[CT]CG[GA]G 638 CCCGAG CelII 540 GCTAAGC GC^T[ACGT]AGC 768 GCTGAGC Cfr13I 112 GGTCC G^G[ACGT]CC 758 GGGCC Hin1I 56 GACGCC G[GA]^CG[CT]C 502 GACGTC HindIII 98 AAGCTT A^AGCTT 874 AAGCTT MboII 195 GAAGA GAAGA(8/7) 744 GAAGA MluNI 380 TGGCCA TGG^CCA 683 TGGCCA MscI 380 TGGCCA TGG^CCA 683 TGGCCA MwoI 676 GCTGCCTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 696 GCAGCACGAGC NlaIV 44 GGATCC GG[ACGT]^[ACGT]CC 780 GGTGCC Sau96I 112 GGTCC G^G[ACGT]CC 758 GGGCC SfcI 712 CTACAG C^T[GA][CT]AG 903 CTACAG Tsp509I 10 AATT ^AATT 131 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BstYI Cac8I CfoI DdeI DpnII EaeI EcoT14I Fnu4HI HaeII HaeIII HhaI HinP1I HinfI ItaI MaeII MboI MflI MnlI NdeII NlaIII RsaI Sau3AI SfaNI StyI TaqI TfiI TseI TthHB8I XhoII