Restriction map for
pF1KB0437
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspI AviII
AvrII BbeI BbrPI BbvCI BcgI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I FseI FspI HaeII
HgaI Hin1I HincII HindII HpaI
HphI KasI KspI MamI MfeI
MluI MroI MunI NarI NcoI
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Tth111I Van91I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 613 GGTACC G^GTACC
AccII 32 CGCG CG^CG
AflIII 821 ACGTGT A^C[GA][CT]GT
Asp718I 613 GGTACC G^GTACC
AvaI 138 CCCGAG C^[CT]CG[GA]G
BalI 474 TGGCCA TGG^CCA
BamHI 359 GGATCC G^GATCC
BbsI 817 GAAGAC GAAGAC(2/6)
BciVI 1026 GGATAC GTATCC(6/5)
BclI 1205 TGATCA T^GATCA
BcnI 105 CCCGG CC^[GC]GG
BpuAI 817 GAAGAC GAAGAC(2/6)
BsaI 995 GAGACC GGTCTC(1/5)
BsoBI 138 CCCGAG C^[CT]CG[GA]G
BspMI 1129 ACCTGC ACCTGC(4/8)
BsrFI 1348 GCCGGC [GA]^CCGG[CT]
BstUI 32 CGCG CG^CG
CfoI 303 GCGC GCG^C
Cfr10I 1348 GCCGGC [GA]^CCGG[CT]
DrdI 181 GACAGTCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1011 ATGCAT ATGCA^T
FauI 1389 CCCGC CCCGC(4/6)
FbaI 1205 TGATCA T^GATCA
HhaI 303 GCGC GCG^C
HinP1I 303 GCGC G^CGC
HindIII 884 AAGCTT A^AGCTT
KpnI 613 GGTACC GGTAC^C
MluNI 474 TGGCCA TGG^CCA
MscI 474 TGGCCA TGG^CCA
MslI 1222 CACATACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 32 CGCG CG^CG
NaeI 1348 GCCGGC GCC^GGC
NciI 105 CCCGG CC^[GC]GG
NgoMIV 1348 GCCGGC G^CCGGC
NsiI 1011 ATGCAT ATGCA^T
PstI 698 CTGCAG CTGCA^G
SapI 865 GCTCTTC GCTCTTC(1/4)
StuI 8 AGGCCT AGG^CCT
TaqI 1044 TCGA T^CGA
TthHB8I 1044 TCGA T^CGA
XbaI 555 TCTAGA T^CTAGA
XcmI 152 CCATCCCCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Asp700I 835 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
862 GAAGCTCTTC
AvaII 135 GGACC G^G[AT]CC
1081 GGACC
BanI 613 GGTACC G^G[CT][GA]CC
1375 GGCACC
BfaI 187 CTAG C^TAG
556 CTAG
BglII 837 AGATCT A^GATCT
1319 AGATCT
BsrDI 940 CATTGC GCAATG(2/0)
665 GCAATG
BstF5I 153 CATCC GGATG(2/0)
1286 GGATG
DraII 134 AGGACCC [GA]G^G[ACGT]CC[CT]
1080 GGGACCT
DraIII 432 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
445 CACTTTGTG
EaeI 474 TGGCCA [CT]^GGCC[GA]
1343 TGGCCG
EcoO109I 134 AGGACCC [GA]G^G[ACGT]CC[CT]
1080 GGGACCT
FokI 153 CATCC GGATG(9/13)
1286 GGATG
HapII 106 CCGG C^CGG
1349 CCGG
HpaII 106 CCGG C^CGG
1349 CCGG
MaeI 187 CTAG C^TAG
556 CTAG
MseI 1061 TTAA T^TAA
1165 TTAA
MspI 106 CCGG C^CGG
1349 CCGG
NdeI 1014 CATATG CA^TATG
1199 CATATG
NspI 299 ACATGC [GA]CATG^[CT]
509 ACATGC
PpuMI 134 AGGACCC [GA]G^G[AT]CC[CT]
1080 GGGACCT
PvuII 97 CAGCTG CAG^CTG
368 CAGCTG
SacI 632 GAGCTC GAGCT^C
1046 GAGCTC
SfcI 283 CTACAG C^T[GA][CT]AG
698 CTGCAG
Tru9I 1061 TTAA T^TAA
1165 TTAA
XmnI 835 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
862 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanII BbvI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstNI BstYI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI HaeIII HinfI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI TseI
Tsp45I Tsp509I TspRI XhoII