Restriction map for
pF1KB6688
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# NO CUT ENZYMES ( 120 enzymes) #
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Acc65I AccIII AclI AflII AflIII
AlwNI Aor51HI ApaI AscI AseI
AsnI Asp718I AvaI AviII BamHI
BbeI BbrPI BbsI BbvCI BciVI
BfrI BglI BglII BlpI Bpu1102I
BpuAI BsaBI BsaI BseAI BseRI
BsiWI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI BstAPI BstEII BstPI BstXI
BstYI CelII ClaI CpoI DraI
DrdI DsaI Eco47III EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII KasI KpnI KspI
MamI MfeI MflI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflMI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SpeI SphI Sse8387I
SspBI StuI SwaI Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AatII 881 GACGTC GACGT^C
AccI 1101 GTATAC GT^[AC][GT]AC
AccII 500 CGCG CG^CG
AcyI 881 GACGTC G[GA]^CG[CT]C
AfaI 154 GTAC GT^AC
AgeI 1263 ACCGGT A^CCGGT
AhdI 308 GACTACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 37 GTGCAC G^TGCAC
AlwI 450 GGATC GGATC(4/5)
AocI 9 CCTGAGG CC^T[ACGT]AGG
ApaLI 37 GTGCAC G^TGCAC
AspEI 308 GACTACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 773 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 653 GGTCC G^G[AT]CC
AvrII 886 CCTAGG C^CTAGG
BcgI 614 CGACGTGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1294 CCCGG CC^[GC]GG
BlnI 886 CCTAGG C^CTAGG
BpmI 1341 CTCCAG CTGGAG(16/14)
BsaHI 881 GACGTC G[GA]^CG[CT]C
BsaWI 1263 ACCGGT [AT]^CCGG[AT]
BsiEI 812 CGGCCG CG[GA][CT]^CG
BsmBI 879 GAGACG CGTCTC(1/5)
BsrDI 288 CATTGC GCAATG(2/0)
BsrFI 1263 ACCGGT [GA]^CCGG[CT]
Bst1107I 1101 GTATAC GTA^TAC
BstBI 927 TTCGAA TT^CGAA
BstUI 500 CGCG CG^CG
BstZ17I 1101 GTATAC GTA^TAC
Bsu36I 9 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1263 ACCGGT [GA]^CCGG[CT]
Cfr13I 653 GGTCC G^G[ACGT]CC
DraII 652 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 612 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 812 CGGCCG C^GGCCG
Eam1105I 308 GACTACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 820 GAAGAG CTCTTC(1/4)
EclXI 812 CGGCCG C^GGCCG
Eco52I 812 CGGCCG C^GGCCG
Eco81I 9 CCTGAGG CC^T[ACGT]AGG
EcoO109I 652 GGGTCCT [GA]G^G[ACGT]CC[CT]
Hin1I 881 GACGTC G[GA]^CG[CT]C
HincII 1237 GTTAAC GT[CT]^[GA]AC
HindII 1237 GTTAAC GT[CT]^[GA]AC
HpaI 1237 GTTAAC GTT^AAC
Ksp632I 820 GAAGAG CTCTTC(1/4)
MspA1I 676 CCGCTG C[AC]G^C[GT]G
MvnI 500 CGCG CG^CG
MwoI 1204 GCCGCCACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 1294 CCCGG CC^[GC]GG
NspV 927 TTCGAA TT^CGAA
PflFI 773 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1263 ACCGGT A^CCGGT
PpuMI 652 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 154 GTAC GT^AC
Sau96I 653 GGTCC G^G[ACGT]CC
SfaNI 1305 GATGC GCATC(5/9)
SfuI 927 TTCGAA TT^CGAA
SspI 991 AATATT AAT^ATT
Tsp45I 1283 GTGAC ^GT[GC]AC
Tth111I 773 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
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Asp700I 920 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1039 GAAAAGCTTC
AspHI 37 GTGCAC G[AT]GC[AT]^C
622 GTGCTC
BalI 163 TGGCCA TGG^CCA
358 TGGCCA
BanI 892 GGCACC G^G[CT][GA]CC
971 GGTGCC
BanII 81 GAGCCC G[GA]GC[CT]^C
1291 GAGCCC
BbvI 91 GCAGC GCAGC(8/12)
797 GCTGC
BclI 221 TGATCA T^GATCA
962 TGATCA
BsaAI 467 TACGTA [CT]AC^GT[GA]
696 TACGTA
BsgI 250 CTGCAC GTGCAG(16/14)
745 CTGCAC
BsiHKAI 37 GTGCAC G[AT]GC[AT]^C
622 GTGCTC
BsmAI 879 GAGAC GTCTC(1/5)
327 GTCTC
BspHI 194 TCATGA T^CATGA
515 TCATGA
BstF5I 213 CATCC GGATG(2/0)
564 CATCC
BstNI 333 CCTGG CC^[AT]GG
769 CCTGG
CfoI 437 GCGC GCG^C
723 GCGC
EcoRII 333 CCTGG ^CC[AT]GG
769 CCTGG
EcoT14I 886 CCTAGG C^C[AT][AT]GG
936 CCTTGG
FbaI 221 TGATCA T^GATCA
962 TGATCA
FokI 213 CATCC GGATG(9/13)
564 CATCC
HapII 1264 CCGG C^CGG
1295 CCGG
HgaI 131 GACGC GACGC(5/10)
418 GCGTC
HhaI 437 GCGC GCG^C
723 GCGC
HinP1I 437 GCGC G^CGC
723 GCGC
HinfI 1085 GAATC G^A[ACGT]TC
1191 GATTC
HpaII 1264 CCGG C^CGG
1295 CCGG
MluNI 163 TGGCCA TGG^CCA
358 TGGCCA
MscI 163 TGGCCA TGG^CCA
358 TGGCCA
MspI 1264 CCGG C^CGG
1295 CCGG
MvaI 333 CCTGG CC^[AT]GG
769 CCTGG
RcaI 194 TCATGA T^CATGA
515 TCATGA
SfcI 95 CTATAG C^T[GA][CT]AG
545 CTATAG
SnaBI 467 TACGTA TAC^GTA
696 TACGTA
StyI 886 CCTAGG C^C[AT][AT]GG
936 CCTTGG
TaqI 928 TCGA T^CGA
1271 TCGA
TfiI 1085 GAATC G^A[AT]TC
1191 GATTC
TseI 91 GCAGC G^C[AT]GC
797 GCTGC
TthHB8I 928 TCGA T^CGA
1271 TCGA
XmnI 920 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1039 GAAAAGCTTC
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AcsI AluI ApoI BfaI
BmyI BsaJI BsiYI BslI BsmFI
BsmI Bsp1286I BsrI BssKI Cac8I
DdeI DpnII EaeI Fnu4HI HaeIII
HindIII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI NdeII NlaIII NlaIV Sau3AI
ScrFI Tru9I Tsp509I TspRI