Restriction map for
pF1KB8532
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AhdI AlwNI AocI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI BciVI BclI BfrI BglII
BsaBI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoT22I FbaI FseI
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SapI SbfI ScaI
SexAI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 448 GTCGAC GT^[AC][GT]AC
AgeI 2184 ACCGGT A^CCGGT
Alw44I 708 GTGCAC G^TGCAC
Aor51HI 1670 AGCGCT AGC^GCT
ApaLI 708 GTGCAC G^TGCAC
AviII 951 TGCGCA TGC^GCA
AvrII 999 CCTAGG C^CTAGG
BalI 395 TGGCCA TGG^CCA
BamHI 2404 GGATCC G^GATCC
BbeI 1987 GGCGCC GGCGC^C
BbrPI 1366 CACGTG CAC^GTG
BbvCI 1447 GCTGAGG CCTCAGC(-5/-2)
BglI 2453 GCCGAAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 999 CCTAGG C^CTAGG
BlpI 1858 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1858 GCTAAGC GC^T[ACGT]AGC
BsaAI 1366 CACGTG [CT]AC^GT[GA]
BsaWI 2184 ACCGGT [AT]^CCGG[AT]
BsgI 1363 CTGCAC GTGCAG(16/14)
BsrBI 115 GAGCGG CCGCTC(-3/-3)
BstAPI 957 GCACGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1858 GCTAAGC GC^T[ACGT]AGC
DraIII 944 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1670 AGCGCT AGC^GCT
EcoRV 937 GATATC GAT^ATC
FspI 951 TGCGCA TGC^GCA
HindIII 1771 AAGCTT A^AGCTT
KasI 1987 GGCGCC G^GCGCC
MluNI 395 TGGCCA TGG^CCA
MscI 395 TGGCCA TGG^CCA
MseI 1376 TTAA T^TAA
MspA1I 1231 CAGCTG C[AC]G^C[GT]G
NarI 1987 GGCGCC GG^CGCC
PinAI 2184 ACCGGT A^CCGGT
PmaCI 1366 CACGTG CAC^GTG
PmlI 1366 CACGTG CAC^GTG
PstI 1246 CTGCAG CTGCA^G
PvuII 1231 CAGCTG CAG^CTG
SalI 448 GTCGAC G^TCGAC
SfoI 1987 GGCGCC GGC^GCC
SgrAI 2183 CACCGGTG C[GA]^CCGG[CT]G
TfiI 1167 GATTC G^A[AT]TC
Tru9I 1376 TTAA T^TAA
XbaI 915 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 715 CGCG CG^CG
1969 CGCG
ApaI 1273 GGGCCC GGGCC^C
1339 GGGCCC
BanI 1987 GGCGCC G^G[CT][GA]CC
2187 GGTGCC
BbsI 240 GAAGAC GAAGAC(2/6)
421 GAAGAC
BcgI 450 CGACGATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1319 GCAATTCATTCG
BpuAI 240 GAAGAC GAAGAC(2/6)
421 GAAGAC
BsaI 1453 GAGACC GGTCTC(1/5)
1648 GAGACC
BsrFI 2014 GCCGGT [GA]^CCGG[CT]
2184 ACCGGT
BstUI 715 CGCG CG^CG
1969 CGCG
BstXI 1298 CCATGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1710 CCAAGGTGCTGG
Cfr10I 2014 GCCGGT [GA]^CCGG[CT]
2184 ACCGGT
DsaI 1995 CCATGG C^C[GA][CT]GG
2346 CCATGG
EcoNI 306 CCTACTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
372 CCTGGACAAGG
HincII 448 GTCGAC GT[CT]^[GA]AC
571 GTCAAC
HindII 448 GTCGAC GT[CT]^[GA]AC
571 GTCAAC
HphI 2167 GGTGA GGTGA(8/7)
2182 TCACC
MvnI 715 CGCG CG^CG
1969 CGCG
NcoI 1995 CCATGG C^CATGG
2346 CCATGG
PaeR7I 751 CTCGAG C^TCGAG
1425 CTCGAG
PflMI 36 CCATGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
93 CCAGAACATGG
PpuMI 701 GGGACCT [GA]G^G[AT]CC[CT]
729 GGGTCCC
SacI 754 GAGCTC GAGCT^C
1636 GAGCTC
SmaI 2389 CCCGGG CCC^GGG
2592 CCCGGG
StuI 1331 AGGCCT AGG^CCT
1742 AGGCCT
Tsp45I 2168 GTGAC ^GT[GC]AC
2505 GTCAC
Van91I 36 CCATGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
93 CCAGAACATGG
XhoI 751 CTCGAG C^TCGAG
1425 CTCGAG
XmaCI 2389 CCCGGG C^CCGGG
2592 CCCGGG
XmaI 2389 CCCGGG C^CCGGG
2592 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TseI Tsp509I TspRI
TthHB8I XcmI XhoII