################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI Asp700I AspEI AspHI AspI AvaI AvaII AviII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BglI BglII BlpI BpmI Bpu1102I BsaBI BsaI BseAI BsgI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssSI Bst1107I BstBI BstEII BstF5I BstNI BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HincII HindII HindIII HpaI KasI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvaI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 309 GGTACC G^GTACC AccII 18 CGCG CG^CG AciI 55 GCGG CCGC(-3/-1) AcyI 94 GACGCC G[GA]^CG[CT]C AflII 319 CTTAAG C^TTAAG AlwI 583 GGATC GGATC(4/5) ApaI 10 GGGCCC GGGCC^C AseI 351 ATTAAT AT^TAAT AsnI 351 ATTAAT AT^TAAT Asp718I 309 GGTACC G^GTACC AvrII 174 CCTAGG C^CTAGG BanI 309 GGTACC G^G[CT][GA]CC BanII 10 GGGCCC G[GA]GC[CT]^C BbvI 473 GCTGC GCAGC(8/12) BfrI 319 CTTAAG C^TTAAG BlnI 174 CCTAGG C^CTAGG BsaAI 112 TACGTG [CT]AC^GT[GA] BsaHI 94 GACGCC G[GA]^CG[CT]C BsaWI 312 ACCGGA [AT]^CCGG[AT] BsiEI 191 CGGCCG CG[GA][CT]^CG BspHI 77 TCATGA T^CATGA BssHII 17 GCGCGC G^CGCGC BstAPI 62 GCAAGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 18 CGCG CG^CG DsaI 152 CCGTGG C^C[GA][CT]GG EaeI 191 CGGCCG [CT]^GGCC[GA] EagI 191 CGGCCG C^GGCCG EclXI 191 CGGCCG C^GGCCG Eco52I 191 CGGCCG C^GGCCG EcoT14I 174 CCTAGG C^C[AT][AT]GG Fnu4HI 473 GCTGC GC^[ACGT]GC Hin1I 94 GACGCC G[GA]^CG[CT]C HinfI 179 GACTC G^A[ACGT]TC HphI 149 TCACC GGTGA(8/7) ItaI 473 GCTGC GC^[ACGT]GC KpnI 309 GGTACC GGTAC^C MaeIII 148 GTCAC ^GT[ACGT]AC MvnI 18 CGCG CG^CG NspI 469 GCATGC [GA]CATG^[CT] PleI 179 GACTC GAGTC(4/5) PshAI 240 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 351 ATTAAT AT^TAAT RcaI 77 TCATGA T^CATGA SfaNI 24 GATGC GCATC(5/9) SphI 469 GCATGC GCATG^C StuI 220 AGGCCT AGG^CCT StyI 174 CCTAGG C^C[AT][AT]GG TaqI 128 TCGA T^CGA TseI 473 GCTGC G^C[AT]GC Tsp45I 148 GTCAC ^GT[GC]AC Tsp509I 609 AATT ^AATT TspRI 564 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 128 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AluI 82 AGCT AG^CT 492 AGCT BcnI 13 CCCGG CC^[GC]GG 102 CCCGG BfaI 175 CTAG C^TAG 451 CTAG BmyI 10 GGGCCC G[AGT]GC[ACT]^C 115 GTGCCC BsaJI 152 CCGTGG C^C[ACGT][ACGT]GG 174 CCTAGG BseRI 106 GAGGAG GAGGAG(10/8) 301 GAGGAG Bsp1286I 10 GGGCCC G[AGT]GC[ACT]^C 115 GTGCCC BssKI 13 CCCGG ^CC[ACGT]GG 102 CCCGG CfoI 17 GCGC GCG^C 19 GCGC Cfr13I 10 GGGCC G^G[ACGT]CC 11 GGCCC DdeI 217 CTGAG C^T[ACGT]AG 362 CTCAG DpnII 370 GATC ^GATC 584 GATC HgaI 94 GACGC GACGC(5/10) 146 GCGTC HhaI 17 GCGC GCG^C 19 GCGC HinP1I 17 GCGC G^CGC 19 GCGC MaeI 175 CTAG C^TAG 451 CTAG MaeII 67 ACGT A^CGT 113 ACGT MboI 370 GATC ^GATC 584 GATC NciI 13 CCCGG CC^[GC]GG 102 CCCGG NdeII 370 GATC ^GATC 584 GATC NlaIII 78 CATG CATG^ 470 CATG NlaIV 10 GGGCCC GG[ACGT]^[ACGT]CC 309 GGTACC Sau3AI 370 GATC ^GATC 584 GATC Sau96I 10 GGGCC G^G[ACGT]CC 11 GGCCC ScrFI 13 CCCGG CC^[ACGT]GG 102 CCCGG SmlI 28 CTCAAG C^T[CT][GA]AG 319 CTTAAG ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AfaI BbsI BpuAI Cac8I HaeIII HapII HpaII MboII MnlI MseI MspI MwoI RsaI Tru9I