Restriction map for pF1KB6915
##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaLI       ApoI        AscI        
Asp700I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       BalI        BamHI       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BglI        BglII       BlpI        BpmI        Bpu1102I    
BsaBI       BsaI        BseAI       BsgI        BsiHKAI     
BsiWI       BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssSI       Bst1107I    
BstBI       BstEII      BstF5I      BstNI       BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        Eam1105I    EarI        Eco47III    
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeII       HincII      
HindII      HindIII     HpaI        KasI        Ksp632I     
KspI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MunI        MvaI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
TfiI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I        309  GGTACC               G^GTACC
AccII          18  CGCG                 CG^CG
AciI           55  GCGG                 CCGC(-3/-1)
AcyI           94  GACGCC               G[GA]^CG[CT]C
AflII         319  CTTAAG               C^TTAAG
AlwI          583  GGATC                GGATC(4/5)
ApaI           10  GGGCCC               GGGCC^C
AseI          351  ATTAAT               AT^TAAT
AsnI          351  ATTAAT               AT^TAAT
Asp718I       309  GGTACC               G^GTACC
AvrII         174  CCTAGG               C^CTAGG
BanI          309  GGTACC               G^G[CT][GA]CC
BanII          10  GGGCCC               G[GA]GC[CT]^C
BbvI          473  GCTGC                GCAGC(8/12)
BfrI          319  CTTAAG               C^TTAAG
BlnI          174  CCTAGG               C^CTAGG
BsaAI         112  TACGTG               [CT]AC^GT[GA]
BsaHI          94  GACGCC               G[GA]^CG[CT]C
BsaWI         312  ACCGGA               [AT]^CCGG[AT]
BsiEI         191  CGGCCG               CG[GA][CT]^CG
BspHI          77  TCATGA               T^CATGA
BssHII         17  GCGCGC               G^CGCGC
BstAPI         62  GCAAGACGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          18  CGCG                 CG^CG
DsaI          152  CCGTGG               C^C[GA][CT]GG
EaeI          191  CGGCCG               [CT]^GGCC[GA]
EagI          191  CGGCCG               C^GGCCG
EclXI         191  CGGCCG               C^GGCCG
Eco52I        191  CGGCCG               C^GGCCG
EcoT14I       174  CCTAGG               C^C[AT][AT]GG
Fnu4HI        473  GCTGC                GC^[ACGT]GC
Hin1I          94  GACGCC               G[GA]^CG[CT]C
HinfI         179  GACTC                G^A[ACGT]TC
HphI          149  TCACC                GGTGA(8/7)
ItaI          473  GCTGC                GC^[ACGT]GC
KpnI          309  GGTACC               GGTAC^C
MaeIII        148  GTCAC                ^GT[ACGT]AC
MvnI           18  CGCG                 CG^CG
NspI          469  GCATGC               [GA]CATG^[CT]
PleI          179  GACTC                GAGTC(4/5)
PshAI         240  GACCGATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         351  ATTAAT               AT^TAAT
RcaI           77  TCATGA               T^CATGA
SfaNI          24  GATGC                GCATC(5/9)
SphI          469  GCATGC               GCATG^C
StuI          220  AGGCCT               AGG^CCT
StyI          174  CCTAGG               C^C[AT][AT]GG
TaqI          128  TCGA                 T^CGA
TseI          473  GCTGC                G^C[AT]GC
Tsp45I        148  GTCAC                ^GT[GC]AC
Tsp509I       609  AATT                 ^AATT
TspRI         564  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       128  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AluI           82  AGCT                 AG^CT
              492  AGCT                
BcnI           13  CCCGG                CC^[GC]GG
              102  CCCGG               
BfaI          175  CTAG                 C^TAG
              451  CTAG                
BmyI           10  GGGCCC               G[AGT]GC[ACT]^C
              115  GTGCCC              
BsaJI         152  CCGTGG               C^C[ACGT][ACGT]GG
              174  CCTAGG              
BseRI         106  GAGGAG               GAGGAG(10/8)
              301  GAGGAG              
Bsp1286I       10  GGGCCC               G[AGT]GC[ACT]^C
              115  GTGCCC              
BssKI          13  CCCGG                ^CC[ACGT]GG
              102  CCCGG               
CfoI           17  GCGC                 GCG^C
               19  GCGC                
Cfr13I         10  GGGCC                G^G[ACGT]CC
               11  GGCCC               
DdeI          217  CTGAG                C^T[ACGT]AG
              362  CTCAG               
DpnII         370  GATC                 ^GATC
              584  GATC                
HgaI           94  GACGC                GACGC(5/10)
              146  GCGTC               
HhaI           17  GCGC                 GCG^C
               19  GCGC                
HinP1I         17  GCGC                 G^CGC
               19  GCGC                
MaeI          175  CTAG                 C^TAG
              451  CTAG                
MaeII          67  ACGT                 A^CGT
              113  ACGT                
MboI          370  GATC                 ^GATC
              584  GATC                
NciI           13  CCCGG                CC^[GC]GG
              102  CCCGG               
NdeII         370  GATC                 ^GATC
              584  GATC                
NlaIII         78  CATG                 CATG^
              470  CATG                
NlaIV          10  GGGCCC               GG[ACGT]^[ACGT]CC
              309  GGTACC              
Sau3AI        370  GATC                 ^GATC
              584  GATC                
Sau96I         10  GGGCC                G^G[ACGT]CC
               11  GGCCC               
ScrFI          13  CCCGG                CC^[ACGT]GG
              102  CCCGG               
SmlI           28  CTCAAG               C^T[CT][GA]AG
              319  CTTAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AfaI        BbsI        BpuAI       Cac8I       HaeIII      
HapII       HpaII       MboII       MnlI        MseI        
MspI        MwoI        RsaI        Tru9I       
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