Restriction map for
pF1KB5954
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvrII BamHI BbeI
BbsI BbvCI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BseAI BsgI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstUI BstXI BstYI
CelII ClaI CpoI DraII EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoRI EcoT22I FauI FseI HaeII
HgaI Hin1I HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MroI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PmeI PpuMI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacII
SapI ScaI SfiI SfoI SfuI
SgrAI SmlI SpeI SphI SspBI
StuI SwaI Tth111I Van91I XbaI
XhoI XhoII
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# ONE CUT ENZYMES ( 70 enzymes) #
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AciI 481 GCGG CCGC(-3/-1)
AgeI 1055 ACCGGT A^CCGGT
AocI 1357 CCTGAGG CC^T[ACGT]AGG
AseI 1291 ATTAAT AT^TAAT
AsnI 1291 ATTAAT AT^TAAT
Asp700I 115 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1126 GAGCTC G[AT]GC[AT]^C
AvaI 236 CCCGGG C^[CT]CG[GA]G
AvaII 399 GGTCC G^G[AT]CC
AviII 410 TGCGCA TGC^GCA
BalI 588 TGGCCA TGG^CCA
BanII 1126 GAGCTC G[GA]GC[CT]^C
BbrPI 1009 CACGTG CAC^GTG
BcgI 1600 CGAGACGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 656 TGATCA T^GATCA
BfaI 7 CTAG C^TAG
BmyI 1126 GAGCTC G[AGT]GC[ACT]^C
BsaI 913 GAGACC GGTCTC(1/5)
BsaWI 1055 ACCGGT [AT]^CCGG[AT]
BsiHKAI 1126 GAGCTC G[AT]GC[AT]^C
BsmFI 1532 GTCCC GGGAC(10/14)
BsmI 548 GAATGC GAATGC(1/-1)
BsoBI 236 CCCGGG C^[CT]CG[GA]G
Bsp1286I 1126 GAGCTC G[AGT]GC[ACT]^C
BsrFI 1055 ACCGGT [GA]^CCGG[CT]
Bst1107I 1503 GTATAC GTA^TAC
BstEII 753 GGTCACC G^GT[ACGT]ACC
BstPI 753 GGTCACC G^GT[ACGT]ACC
BstZ17I 1503 GTATAC GTA^TAC
Bsu36I 1357 CCTGAGG CC^T[ACGT]AGG
CfoI 411 GCGC GCG^C
Cfr10I 1055 ACCGGT [GA]^CCGG[CT]
DraI 76 TTTAAA TTT^AAA
DraIII 756 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 673 GACGTTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 588 TGGCCA [CT]^GGCC[GA]
Eco81I 1357 CCTGAGG CC^T[ACGT]AGG
EcoNI 634 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 753 GGTCACC G^GT[ACGT]ACC
EcoRV 1252 GATATC GAT^ATC
FbaI 656 TGATCA T^GATCA
FspI 410 TGCGCA TGC^GCA
HhaI 411 GCGC GCG^C
HinP1I 411 GCGC G^CGC
HincII 1384 GTCGAC GT[CT]^[GA]AC
HindII 1384 GTCGAC GT[CT]^[GA]AC
HindIII 856 AAGCTT A^AGCTT
MaeI 7 CTAG C^TAG
MluNI 588 TGGCCA TGG^CCA
MscI 588 TGGCCA TGG^CCA
MslI 1005 CACTCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 6 GCTAGC G^CTAGC
PinAI 1055 ACCGGT A^CCGGT
PmaCI 1009 CACGTG CAC^GTG
PmlI 1009 CACGTG CAC^GTG
PshBI 1291 ATTAAT AT^TAAT
PstI 1106 CTGCAG CTGCA^G
SacI 1126 GAGCTC GAGCT^C
SalI 1384 GTCGAC G^TCGAC
SbfI 1105 CCTGCAGG CCTGCA^GG
SexAI 757 ACCAGGT A^CC[AT]GGT
SfcI 1106 CTGCAG C^T[GA][CT]AG
SmaI 236 CCCGGG CCC^GGG
SnaBI 133 TACGTA TAC^GTA
Sse8387I 1105 CCTGCAGG CCTGCA^GG
SspI 1092 AATATT AAT^ATT
XcmI 1536 CCATGGAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 236 CCCGGG C^CCGGG
XmaI 236 CCCGGG C^CCGGG
XmnI 115 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 1384 GTCGAC GT^[AC][GT]AC
1503 GTATAC
AcsI 426 GAATTT [GA]^AATT[CT]
1240 GAATTT
ApoI 426 GAATTT [GA]^AATT[CT]
1240 GAATTT
BanI 841 GGTGCC G^G[CT][GA]CC
989 GGCACC
BbvI 1347 GCAGC GCAGC(8/12)
408 GCTGC
BpmI 581 CTCCAG CTGGAG(16/14)
638 CTGGAG
BsaAI 133 TACGTA [CT]AC^GT[GA]
1009 CACGTG
BsmBI 21 CGTCTC CGTCTC(1/5)
1601 GAGACG
BspLU11I 722 ACATGT A^CATGT
1528 ACATGT
BsrI 1455 ACTGG ACTGG(1/-1)
1499 ACTGG
Cfr13I 399 GGTCC G^G[ACGT]CC
630 GGCCC
Fnu4HI 408 GCTGC GC^[ACGT]GC
1347 GCAGC
ItaI 408 GCTGC GC^[ACGT]GC
1347 GCAGC
NcoI 749 CCATGG C^CATGG
1536 CCATGG
PleI 888 GACTC GAGTC(4/5)
667 GAGTC
Sau96I 399 GGTCC G^G[ACGT]CC
630 GGCCC
TseI 408 GCTGC G^C[AT]GC
1347 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AfaI AflIII AluI AlwI BcnI
BsaJI BseRI BsiYI BslI BsmAI
BspMI BssKI BstF5I BstNI Cac8I
DdeI DpnII DsaI EarI EcoRII
EcoT14I FokI HaeIII HapII HinfI
HpaII HphI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI RsaI Sau3AI ScrFI
SfaNI StyI TaqI TfiI Tru9I
Tsp45I Tsp509I TspRI TthHB8I