Restriction map for
pF1KB6421
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaII AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BciVI
BclI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiWI
BsmBI BsmI Bsp1407I BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
Hin1I HincII HindII HpaI HphI
KasI KpnI Ksp632I KspI MaeIII
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I XcmI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AfaI 917 GTAC GT^AC
AflII 535 CTTAAG C^TTAAG
AflIII 646 ACACGT A^C[GA][CT]GT
AspHI 709 GAGCAC G[AT]GC[AT]^C
BalI 144 TGGCCA TGG^CCA
BanI 30 GGTGCC G^G[CT][GA]CC
BbsI 393 GAAGAC GAAGAC(2/6)
BcgI 106 GCAGTGCGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 535 CTTAAG C^TTAAG
BpuAI 393 GAAGAC GAAGAC(2/6)
BseRI 127 GAGGAG GAGGAG(10/8)
BsiEI 112 CGATCG CG[GA][CT]^CG
BsiHKAI 709 GAGCAC G[AT]GC[AT]^C
BsiYI 406 CCAGGTCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 406 CCAGGTCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 338 GAGAC GTCTC(1/5)
BspDI 114 ATCGAT AT^CGAT
BstAPI 346 GCAGTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr13I 56 GGGCC G^G[ACGT]CC
ClaI 114 ATCGAT AT^CGAT
EaeI 144 TGGCCA [CT]^GGCC[GA]
EcoRI 427 GAATTC G^AATTC
HapII 162 CCGG C^CGG
HgaI 166 GACGC GACGC(5/10)
HpaII 162 CCGG C^CGG
MaeII 648 ACGT A^CGT
MluNI 144 TGGCCA TGG^CCA
MscI 144 TGGCCA TGG^CCA
MslI 757 CATCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 162 CCGG C^CGG
NlaIV 30 GGTGCC GG[ACGT]^[ACGT]CC
PflMI 406 CCAGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 112 CGATCG CGAT^CG
PvuII 505 CAGCTG CAG^CTG
RsaI 917 GTAC GT^AC
Sau96I 56 GGGCC G^G[ACGT]CC
SmaI 161 CCCGGG CCC^GGG
SmlI 535 CTTAAG C^T[CT][GA]AG
SpeI 397 ACTAGT A^CTAGT
Van91I 406 CCAGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 751 TCTAGA T^CTAGA
XmaCI 161 CCCGGG C^CCGGG
XmaI 161 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AciI 68 GCGG CCGC(-3/-1)
588 GCGG
AlwI 862 GATCC GGATC(4/5)
748 GGATC
Asp700I 427 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
895 GAAGAAATTC
AvaI 124 CCCGAG C^[CT]CG[GA]G
161 CCCGGG
BcnI 161 CCCGG CC^[GC]GG
162 CCGGG
BmyI 530 GGGCAC G[AGT]GC[ACT]^C
709 GAGCAC
BsmFI 164 GGGAC GGGAC(10/14)
875 GGGAC
BsoBI 124 CCCGAG C^[CT]CG[GA]G
161 CCCGGG
Bsp1286I 530 GGGCAC G[AGT]GC[ACT]^C
709 GAGCAC
BsrI 147 CCAGT ACTGG(1/-1)
274 CCAGT
BstNI 406 CCAGG CC^[AT]GG
657 CCTGG
BstYI 748 GGATCT [GA]^GATC[CT]
861 AGATCC
CfoI 51 GCGC GCG^C
138 GCGC
DsaI 38 CCATGG C^C[GA][CT]GG
179 CCATGG
EcoRII 406 CCAGG ^CC[AT]GG
657 CCTGG
HhaI 51 GCGC GCG^C
138 GCGC
HinP1I 51 GCGC G^CGC
138 GCGC
HindIII 483 AAGCTT A^AGCTT
543 AAGCTT
MflI 748 GGATCT [GA]^GATC[CT]
861 AGATCC
MspA1I 505 CAGCTG C[AC]G^C[GT]G
586 CAGCGG
MvaI 406 CCAGG CC^[AT]GG
657 CCTGG
NciI 161 CCCGG CC^[GC]GG
162 CCGGG
NcoI 38 CCATGG C^CATGG
179 CCATGG
PleI 289 GACTC GAGTC(4/5)
501 GACTC
TaqI 115 TCGA T^CGA
295 TCGA
TspRI 107 CAGTG CA[GC]TG[ACGT][ACGT]^
347 CAGTG
TthHB8I 115 TCGA T^CGA
295 TCGA
XhoII 748 GGATCT [GA]^GATC[CT]
861 AGATCC
XmnI 427 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
895 GAAGAAATTC
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AluI ApoI BbvI BfaI
BsaJI BssKI BstF5I Cac8I DdeI
DpnII EcoT14I Fnu4HI FokI HaeIII
HinfI ItaI MaeI MboI MboII
MnlI MseI MwoI NdeII NlaIII
Sau3AI ScrFI StyI TfiI Tru9I
TseI Tsp509I