Restriction map for
pF1KA0641
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# NO CUT ENZYMES ( 69 enzymes) #
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AclI AcsI AflII AgeI ApoI
AseI AsnI AvrII BamHI BciVI
BclI BfrI BglII BlnI BsaBI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstBI BstYI BstZ17I ClaI DraI
DraIII EcoRI EcoRV EcoT22I FbaI
HpaI MamI MfeI MflI MluI
MseI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RcaI SapI SexAI SfuI SnaBI
SpeI SphI SspBI SspI SwaI
Tru9I XbaI XcmI XhoII
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# ONE CUT ENZYMES ( 32 enzymes) #
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Acc65I 1856 GGTACC G^GTACC
AccIII 2931 TCCGGA T^CCGGA
AlwI 578 GGATC GGATC(4/5)
Aor51HI 2490 AGCGCT AGC^GCT
AscI 1219 GGCGCGCC GG^CGCGCC
Asp700I 102 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1856 GGTACC G^GTACC
AviII 3311 TGCGCA TGC^GCA
BbrPI 3742 CACGTG CAC^GTG
BbsI 2446 GTCTTC GAAGAC(2/6)
BpuAI 2446 GTCTTC GAAGAC(2/6)
BsaAI 3742 CACGTG [CT]AC^GT[GA]
BseAI 2931 TCCGGA T^CCGGA
BsmBI 1785 CGTCTC CGTCTC(1/5)
BspEI 2931 TCCGGA T^CCGGA
DrdI 3400 GACGTCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2490 AGCGCT AGC^GCT
FspI 3311 TGCGCA TGC^GCA
HincII 1441 GTCGAC GT[CT]^[GA]AC
HindII 1441 GTCGAC GT[CT]^[GA]AC
KpnI 1856 GGTACC GGTAC^C
MroI 2931 TCCGGA T^CCGGA
PaeR7I 2692 CTCGAG C^TCGAG
PmaCI 3742 CACGTG CAC^GTG
PmlI 3742 CACGTG CAC^GTG
PshAI 3056 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1441 GTCGAC G^TCGAC
ScaI 1277 AGTACT AGT^ACT
SfiI 1155 GGCCTTCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 3263 CGCCGGCG C[GA]^CCGG[CT]G
XhoI 2692 CTCGAG C^TCGAG
XmnI 102 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
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AatII 1444 GACGTC GACGT^C
3400 GACGTC
AccI 1441 GTCGAC GT^[AC][GT]AC
3409 GTCTAC
AhdI 2410 GACATCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3757 GACGCCGAGTC
Alw44I 439 GTGCAC G^TGCAC
862 GTGCAC
ApaLI 439 GTGCAC G^TGCAC
862 GTGCAC
AspEI 2410 GACATCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3757 GACGCCGAGTC
BalI 2 TGGCCA TGG^CCA
1406 TGGCCA
BlpI 1170 GCTGAGC GC^T[ACGT]AGC
1245 GCTCAGC
Bpu1102I 1170 GCTGAGC GC^T[ACGT]AGC
1245 GCTCAGC
BsaI 1099 GAGACC GGTCTC(1/5)
3346 GAGACC
BstAPI 864 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1888 GCAGCCTCTGC
BstEII 1092 GGTGACC G^GT[ACGT]ACC
2008 GGTGACC
BstPI 1092 GGTGACC G^GT[ACGT]ACC
2008 GGTGACC
CelII 1170 GCTGAGC GC^T[ACGT]AGC
1245 GCTCAGC
CpoI 740 CGGTCCG CG^G[AT]CCG
794 CGGACCG
DpnII 579 GATC ^GATC
3017 GATC
Eam1105I 2410 GACATCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3757 GACGCCGAGTC
EarI 3253 GAAGAG CTCTTC(1/4)
3568 GAAGAG
EcoO65I 1092 GGTGACC G^GT[ACGT]ACC
2008 GGTGACC
FseI 3068 GGCCGGCC GGCCGG^CC
3157 GGCCGGCC
HindIII 1482 AAGCTT A^AGCTT
2032 AAGCTT
Ksp632I 3253 GAAGAG CTCTTC(1/4)
3568 GAAGAG
MboI 579 GATC ^GATC
3017 GATC
MluNI 2 TGGCCA TGG^CCA
1406 TGGCCA
MscI 2 TGGCCA TGG^CCA
1406 TGGCCA
MslI 42 CACAGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3738 CACGCACGTG
NdeII 579 GATC ^GATC
3017 GATC
RsrII 740 CGGTCCG CG^G[AT]CCG
794 CGGACCG
Sau3AI 579 GATC ^GATC
3017 GATC
SbfI 1194 CCTGCAGG CCTGCA^GG
2325 CCTGCAGG
Sse8387I 1194 CCTGCAGG CCTGCA^GG
2325 CCTGCAGG
TfiI 2658 GAATC G^A[AT]TC
3472 GAATC
Tsp509I 433 AATT ^AATT
1117 AATT
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# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI AocI ApaI AspHI
AspI AvaI AvaII BanI BanII
BbeI BbvCI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EaeI EagI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI KspI
MaeI MaeII MaeIII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NgoMIV
NlaIII NlaIV PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TseI Tsp45I TspRI Tth111I
TthHB8I Van91I XmaCI XmaI