Restriction map for
pF1KA0822
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AclI AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspEI BbeI BcgI BciVI
BlpI Bpu1102I BsgI BsiWI BsmBI
BspLU11I BssHII BstEII BstPI CelII
CpoI DraI DraII EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
FseI HgaI HpaI KasI KpnI
KspI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PinAI PmeI PpuMI PshAI
Psp1406I PvuI RsrII SacII SbfI
ScaI SexAI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1289 GACGTC GACGT^C
AccIII 3052 TCCGGA T^CCGGA
AcyI 1289 GACGTC G[GA]^CG[CT]C
AflII 2013 CTTAAG C^TTAAG
AspI 4128 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4228 CCCGAG C^[CT]CG[GA]G
AviII 2416 TGCGCA TGC^GCA
AvrII 2190 CCTAGG C^CTAGG
BalI 4343 TGGCCA TGG^CCA
BanI 4288 GGTGCC G^G[CT][GA]CC
BbrPI 1348 CACGTG CAC^GTG
BfrI 2013 CTTAAG C^TTAAG
BglI 4255 GCCATCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2190 CCTAGG C^CTAGG
BsaHI 1289 GACGTC G[GA]^CG[CT]C
BsaI 1325 GGTCTC GGTCTC(1/5)
BseAI 3052 TCCGGA T^CCGGA
BsiEI 4214 CGACCG CG[GA][CT]^CG
BsoBI 4228 CCCGAG C^[CT]CG[GA]G
BspDI 3010 ATCGAT AT^CGAT
BspEI 3052 TCCGGA T^CCGGA
BsrBI 3933 GAGCGG CCGCTC(-3/-3)
BsrFI 988 ACCGGC [GA]^CCGG[CT]
BssSI 3795 CACGAG CACGAG(-5/-1)
BstAPI 2797 GCATGCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 182 CCATGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 988 ACCGGC [GA]^CCGG[CT]
ClaI 3010 ATCGAT AT^CGAT
DrdI 1283 GACATCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 4343 TGGCCA [CT]^GGCC[GA]
EcoRV 2006 GATATC GAT^ATC
FauI 1966 GCGGG CCCGC(4/6)
FspI 2416 TGCGCA TGC^GCA
Hin1I 1289 GACGTC G[GA]^CG[CT]C
MfeI 2420 CAATTG C^AATTG
MluNI 4343 TGGCCA TGG^CCA
MroI 3052 TCCGGA T^CCGGA
MscI 4343 TGGCCA TGG^CCA
MunI 2420 CAATTG C^AATTG
PflFI 4128 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1348 CACGTG CAC^GTG
PmlI 1348 CACGTG CAC^GTG
PstI 4099 CTGCAG CTGCA^G
SalI 4212 GTCGAC G^TCGAC
SfcI 4099 CTGCAG C^T[GA][CT]AG
SfiI 4254 GGCCATCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2797 GCATGC GCATG^C
StuI 755 AGGCCT AGG^CCT
Tth111I 4128 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 4301 CCACCCACTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AocI 3973 CCTCAGG CC^T[ACGT]AGG
4476 CCTGAGG
BamHI 1821 GGATCC G^GATCC
3573 GGATCC
BclI 1344 TGATCA T^GATCA
2627 TGATCA
BcnI 4217 CCGGG CC^[GC]GG
4260 CCGGG
BglII 865 AGATCT A^GATCT
1500 AGATCT
BsaAI 1348 CACGTG [CT]AC^GT[GA]
3241 TACGTG
BsmI 431 GAATGC GAATGC(1/-1)
633 GCATTC
Bsp1407I 1046 TGTACA T^GTACA
3468 TGTACA
BspHI 1389 TCATGA T^CATGA
3233 TCATGA
BsrGI 1046 TGTACA T^GTACA
3468 TGTACA
Bst1107I 232 GTATAC GTA^TAC
2520 GTATAC
BstBI 3157 TTCGAA TT^CGAA
3349 TTCGAA
BstZ17I 232 GTATAC GTA^TAC
2520 GTATAC
Bsu36I 3973 CCTCAGG CC^T[ACGT]AGG
4476 CCTGAGG
DraIII 583 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2757 CACAGTGTG
DsaI 182 CCATGG C^C[GA][CT]GG
2525 CCATGG
Eco81I 3973 CCTCAGG CC^T[ACGT]AGG
4476 CCTGAGG
FbaI 1344 TGATCA T^GATCA
2627 TGATCA
HaeII 1407 AGCGCC [GA]GCGC^[CT]
3087 GGCGCT
MspA1I 3726 CAGCTG C[AC]G^C[GT]G
4108 CAGCTG
NciI 4217 CCGGG CC^[GC]GG
4260 CCGGG
NcoI 182 CCATGG C^CATGG
2525 CCATGG
NspI 2797 GCATGC [GA]CATG^[CT]
3164 ACATGC
NspV 3157 TTCGAA TT^CGAA
3349 TTCGAA
PvuII 3726 CAGCTG CAG^CTG
4108 CAGCTG
RcaI 1389 TCATGA T^CATGA
3233 TCATGA
SacI 3029 GAGCTC GAGCT^C
4657 GAGCTC
SfuI 3157 TTCGAA TT^CGAA
3349 TTCGAA
SmlI 2013 CTTAAG C^T[CT][GA]AG
4093 CTCAAG
SspBI 1046 TGTACA T^GTACA
3468 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BanII
BbsI BbvCI BbvI BfaI BmyI
BpmI BpuAI BsaBI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoNI EcoRI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NsiI PflMI PleI
PshBI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI