Restriction map for
pF1KB7328
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# NO CUT ENZYMES ( 74 enzymes) #
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AatII AclI AflII AgeI Alw44I
AocI ApaLI AscI AseI AsnI
AspI AviII BbeI BbvCI BcgI
BciVI BfrI BglI BspDI BspLU11I
BssHII Bst1107I BstAPI BstBI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eco81I EcoRI EcoT22I FseI FspI
HpaI KasI KspI MfeI MunI
NarI NcoI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SbfI SexAI SfiI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 2372 GGTACC G^GTACC
AccIII 3472 TCCGGA T^CCGGA
AhdI 1882 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2422 AGCGCT AGC^GCT
Asp700I 3464 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2372 GGTACC G^GTACC
AspEI 1882 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3803 CCCGAG C^[CT]CG[GA]G
AvrII 1578 CCTAGG C^CTAGG
BanI 2372 GGTACC G^G[CT][GA]CC
BlnI 1578 CCTAGG C^CTAGG
BlpI 3195 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3195 GCTGAGC GC^T[ACGT]AGC
BsaBI 372 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3472 TCCGGA T^CCGGA
BsiWI 758 CGTACG C^GTACG
BsoBI 3803 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2561 TGTACA T^GTACA
BspEI 3472 TCCGGA T^CCGGA
BsrGI 2561 TGTACA T^GTACA
BssSI 2821 CTCGTG CACGAG(-5/-1)
CelII 3195 GCTGAGC GC^T[ACGT]AGC
DraIII 337 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1892 CGGCCG C^GGCCG
Eam1105I 1882 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1892 CGGCCG C^GGCCG
Eco47III 2422 AGCGCT AGC^GCT
Eco52I 1892 CGGCCG C^GGCCG
HincII 240 GTCGAC GT[CT]^[GA]AC
HindII 240 GTCGAC GT[CT]^[GA]AC
HindIII 1497 AAGCTT A^AGCTT
KpnI 2372 GGTACC GGTAC^C
MamI 372 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 363 ACGCGT A^CGCGT
MroI 3472 TCCGGA T^CCGGA
NaeI 47 GCCGGC GCC^GGC
NdeI 245 CATATG CA^TATG
NgoMIV 47 GCCGGC G^CCGGC
NspI 2544 GCATGT [GA]CATG^[CT]
SalI 240 GTCGAC G^TCGAC
SapI 3808 GCTCTTC GCTCTTC(1/4)
ScaI 3154 AGTACT AGT^ACT
SgrAI 182 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 2926 CTCAAG C^T[CT][GA]AG
SspBI 2561 TGTACA T^GTACA
XmnI 3464 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcyI 1900 GACGCC G[GA]^CG[CT]C
2596 GGCGTC
AflIII 363 ACGCGT A^C[GA][CT]GT
2348 ACACGT
BbrPI 337 CACGTG CAC^GTG
1906 CACGTG
BclI 932 TGATCA T^GATCA
1701 TGATCA
BcnI 132 CCCGG CC^[GC]GG
146 CCGGG
BsaHI 1900 GACGCC G[GA]^CG[CT]C
2596 GGCGTC
BsaI 1772 GAGACC GGTCTC(1/5)
2449 GGTCTC
BsaWI 575 TCCGGT [AT]^CCGG[AT]
3472 TCCGGA
BsgI 1486 CTGCAC GTGCAG(16/14)
3915 CTGCAC
BsiEI 1857 CGACCG CG[GA][CT]^CG
1892 CGGCCG
BsmBI 1104 GAGACG CGTCTC(1/5)
3235 GAGACG
BsmFI 3322 GGGAC GGGAC(10/14)
3329 GTCCC
BsmI 3298 GAATGC GAATGC(1/-1)
3557 GCATTC
BspHI 3095 TCATGA T^CATGA
3701 TCATGA
BsrBI 1792 GAGCGG CCGCTC(-3/-3)
3198 GAGCGG
EcoRV 798 GATATC GAT^ATC
3550 GATATC
FauI 1841 CCCGC CCCGC(4/6)
1794 GCGGG
FbaI 932 TGATCA T^GATCA
1701 TGATCA
Hin1I 1900 GACGCC G[GA]^CG[CT]C
2596 GGCGTC
NciI 132 CCCGG CC^[GC]GG
146 CCGGG
PflMI 3332 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3517 CCAGCCAGTGG
PmaCI 337 CACGTG CAC^GTG
1906 CACGTG
PmlI 337 CACGTG CAC^GTG
1906 CACGTG
PshAI 1959 GACAGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2533 GACATCGGTC
PstI 1594 CTGCAG CTGCA^G
2791 CTGCAG
RcaI 3095 TCATGA T^CATGA
3701 TCATGA
StuI 323 AGGCCT AGG^CCT
3168 AGGCCT
Van91I 3332 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3517 CCAGCCAGTGG
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# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaII BalI BamHI BanII
BbsI BbvI BfaI BglII BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoO65I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
PpuMI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII