Restriction map for
pF1KB6579
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII BamHI BanII
BbeI BbrPI BbvCI BcgI BclI
BfrI BpmI BsaWI BseAI BsgI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DsaI
Eam1105I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MseI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp509I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Aor51HI 764 AGCGCT AGC^GCT
AspI 475 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 298 CCCGAG C^[CT]CG[GA]G
AvrII 85 CCTAGG C^CTAGG
BalI 407 TGGCCA TGG^CCA
BbsI 10 GAAGAC GAAGAC(2/6)
BciVI 194 GTATCC GTATCC(6/5)
BfaI 86 CTAG C^TAG
BglII 257 AGATCT A^GATCT
BlnI 85 CCTAGG C^CTAGG
BlpI 616 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 616 GCTGAGC GC^T[ACGT]AGC
BpuAI 10 GAAGAC GAAGAC(2/6)
BsiEI 692 CGGCCG CG[GA][CT]^CG
BsmBI 781 GAGACG CGTCTC(1/5)
BsmFI 426 GTCCC GGGAC(10/14)
BsmI 824 GCATTC GAATGC(1/-1)
BsoBI 298 CCCGAG C^[CT]CG[GA]G
BspHI 851 TCATGA T^CATGA
BspMI 741 GCAGGT ACCTGC(4/8)
BsrI 239 ACTGG ACTGG(1/-1)
BstXI 398 CCATGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 616 GCTGAGC GC^T[ACGT]AGC
DraIII 487 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 736 GACGGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 692 CGGCCG C^GGCCG
EarI 787 CTCTTC CTCTTC(1/4)
EclXI 692 CGGCCG C^GGCCG
Eco47III 764 AGCGCT AGC^GCT
Eco52I 692 CGGCCG C^GGCCG
EcoNI 437 CCTCCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 1098 GGCCGGCC GGCCGG^CC
HaeII 764 AGCGCT [GA]GCGC^[CT]
Ksp632I 787 CTCTTC CTCTTC(1/4)
MaeI 86 CTAG C^TAG
MluNI 407 TGGCCA TGG^CCA
MscI 407 TGGCCA TGG^CCA
NspI 941 GCATGC [GA]CATG^[CT]
PflFI 475 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 878 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 614 CAGCTG CAG^CTG
RcaI 851 TCATGA T^CATGA
SapI 786 GCTCTTC GCTCTTC(1/4)
SfiI 408 GGCCATCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 941 GCATGC GCATG^C
TfiI 157 GATTC G^A[AT]TC
TspRI 585 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 475 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 527 CCATCATGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AspHI 220 GAGCAC G[AT]GC[AT]^C
304 GAGCAC
BcnI 71 CCGGG CC^[GC]GG
777 CCCGG
BsiHKAI 220 GAGCAC G[AT]GC[AT]^C
304 GAGCAC
BssSI 829 CACGAG CACGAG(-5/-1)
28 CTCGTG
BstYI 257 AGATCT [GA]^GATC[CT]
578 AGATCC
DraII 326 AGGCCCC [GA]G^G[ACGT]CC[CT]
878 AGGACCT
EaeI 407 TGGCCA [CT]^GGCC[GA]
692 CGGCCG
EcoO109I 326 AGGCCCC [GA]G^G[ACGT]CC[CT]
878 AGGACCT
EcoT14I 85 CCTAGG C^C[AT][AT]GG
341 CCAAGG
MflI 257 AGATCT [GA]^GATC[CT]
578 AGATCC
MspA1I 614 CAGCTG C[AC]G^C[GT]G
771 CCGCTG
NaeI 61 GCCGGC GCC^GGC
1099 GCCGGC
NciI 71 CCGGG CC^[GC]GG
777 CCCGG
NgoMIV 61 GCCGGC G^CCGGC
1099 GCCGGC
PleI 466 GACTC GAGTC(4/5)
814 GAGTC
StyI 85 CCTAGG C^C[AT][AT]GG
341 CCAAGG
XhoII 257 AGATCT [GA]^GATC[CT]
578 AGATCC
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaII BanI BbvI BglI
BmyI BsaAI BsaBI BsaHI BsaI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BsrFI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MamI MboI
MboII MnlI MslI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TseI Tsp45I TthHB8I