Restriction map for
pF1KB6585
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
Acc65I AccI AccII AcsI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BfrI BlnI
BpuAI BseRI BsgI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MseI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 113 GACGTC GACGT^C
AccIII 1002 TCCGGA T^CCGGA
AclI 764 AACGTT AA^CGTT
AcyI 113 GACGTC G[GA]^CG[CT]C
AvaII 879 GGACC G^G[AT]CC
BalI 686 TGGCCA TGG^CCA
BanII 49 GGGCTC G[GA]GC[CT]^C
BbvI 773 GCTGC GCAGC(8/12)
BclI 746 TGATCA T^GATCA
BfaI 964 CTAG C^TAG
BglI 732 GCCTGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 616 GCTGAGC GC^T[ACGT]AGC
BpmI 470 CTGGAG CTGGAG(16/14)
Bpu1102I 616 GCTGAGC GC^T[ACGT]AGC
BsaAI 163 TACGTG [CT]AC^GT[GA]
BsaHI 113 GACGTC G[GA]^CG[CT]C
BsaI 781 GAGACC GGTCTC(1/5)
BsaWI 1002 TCCGGA [AT]^CCGG[AT]
BseAI 1002 TCCGGA T^CCGGA
BsiEI 936 CGACCG CG[GA][CT]^CG
BsmI 941 GAATGC GAATGC(1/-1)
BspEI 1002 TCCGGA T^CCGGA
BsrFI 61 GCCGGC [GA]^CCGG[CT]
BstXI 398 CCATGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 616 GCTGAGC GC^T[ACGT]AGC
CfoI 1008 GCGC GCG^C
Cfr10I 61 GCCGGC [GA]^CCGG[CT]
DraII 878 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 878 AGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 746 TGATCA T^GATCA
HhaI 1008 GCGC GCG^C
Hin1I 113 GACGTC G[GA]^CG[CT]C
HinP1I 1008 GCGC G^CGC
MaeI 964 CTAG C^TAG
MluNI 686 TGGCCA TGG^CCA
MroI 1002 TCCGGA T^CCGGA
MscI 686 TGGCCA TGG^CCA
MspA1I 771 CCGCTG C[AC]G^C[GT]G
NaeI 61 GCCGGC GCC^GGC
NgoMIV 61 GCCGGC G^CCGGC
PpuMI 878 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 764 AACGTT AA^CGTT
SapI 301 GAAGAGC GCTCTTC(1/4)
SmlI 712 CTTGAG C^T[CT][GA]AG
TfiI 194 GAATC G^A[AT]TC
TseI 773 GCTGC G^C[AT]GC
Tsp509I 369 AATT ^AATT
XcmI 527 CCATCATGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 210 GTAC GT^AC
921 GTAC
BglII 257 AGATCT A^GATCT
555 AGATCT
BmyI 49 GGGCTC G[AGT]GC[ACT]^C
909 GGGCAC
Bsp1286I 49 GGGCTC G[AGT]GC[ACT]^C
909 GGGCAC
BspHI 572 TCATGA T^CATGA
851 TCATGA
BsrDI 930 CATTGC GCAATG(2/0)
993 CATTGC
BsrI 239 ACTGG ACTGG(1/-1)
451 ACTGG
DdeI 587 CTGAG C^T[ACGT]AG
617 CTGAG
EaeI 549 TGGCCG [CT]^GGCC[GA]
686 TGGCCA
EcoT14I 26 CCTTGG C^C[AT][AT]GG
341 CCAAGG
Fnu4HI 694 GCCGC GC^[ACGT]GC
773 GCTGC
HgaI 443 GACGC GACGC(5/10)
534 GCGTC
ItaI 694 GCCGC GC^[ACGT]GC
773 GCTGC
RcaI 572 TCATGA T^CATGA
851 TCATGA
RsaI 210 GTAC GT^AC
921 GTAC
StyI 26 CCTTGG C^C[AT][AT]GG
341 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AlwI BanI BcnI
BsaBI BsaJI BsiYI BslI BsmAI
BsmFI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DpnII EarI EcoRII
FokI HaeIII HapII HinfI HpaII
HphI Ksp632I MaeII MaeIII MamI
MboI MboII MflI MnlI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI Tsp45I TspRI XhoII