Restriction map for
pF1KE0358
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AgeI AhdI AlwNI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BbeI BbrPI BbvCI BcgI BglI
BlpI Bpu1102I BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BsmFI
BspDI BspEI BsrBI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII CfoI ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI FauI FseI
FspI HaeII HhaI HinP1I HpaI
KasI KpnI KspI MluI MroI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 1812 GGCGTC G[GA]^CG[CT]C
AflII 1732 CTTAAG C^TTAAG
Alw44I 1416 GTGCAC G^TGCAC
ApaLI 1416 GTGCAC G^TGCAC
AvaI 7 CCCGGG C^[CT]CG[GA]G
AvrII 240 CCTAGG C^CTAGG
BanII 2106 GAGCCC G[GA]GC[CT]^C
BclI 855 TGATCA T^GATCA
BfaI 241 CTAG C^TAG
BfrI 1732 CTTAAG C^TTAAG
BglII 1673 AGATCT A^GATCT
BlnI 240 CCTAGG C^CTAGG
BsaAI 372 TACGTG [CT]AC^GT[GA]
BsaHI 1812 GGCGTC G[GA]^CG[CT]C
BseRI 1648 GAGGAG GAGGAG(10/8)
BsoBI 7 CCCGGG C^[CT]CG[GA]G
Bsp1407I 508 TGTACA T^GTACA
BspMI 2211 ACCTGC ACCTGC(4/8)
BsrDI 1910 GCAATG GCAATG(2/0)
BsrFI 1383 ACCGGC [GA]^CCGG[CT]
BsrGI 508 TGTACA T^GTACA
BssSI 1517 CTCGTG CACGAG(-5/-1)
BstXI 1206 CCAATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1383 ACCGGC [GA]^CCGG[CT]
DsaI 1932 CCACGG C^C[GA][CT]GG
EarI 1240 GAAGAG CTCTTC(1/4)
EcoRV 1570 GATATC GAT^ATC
EcoT22I 1164 ATGCAT ATGCA^T
FbaI 855 TGATCA T^GATCA
HgaI 1813 GCGTC GACGC(5/10)
Hin1I 1812 GGCGTC G[GA]^CG[CT]C
HindIII 100 AAGCTT A^AGCTT
Ksp632I 1240 GAAGAG CTCTTC(1/4)
MaeI 241 CTAG C^TAG
MfeI 1893 CAATTG C^AATTG
MspA1I 356 CAGCTG C[AC]G^C[GT]G
MunI 1893 CAATTG C^AATTG
NdeI 1374 CATATG CA^TATG
NsiI 1164 ATGCAT ATGCA^T
PvuII 356 CAGCTG CAG^CTG
ScaI 1139 AGTACT AGT^ACT
SmaI 7 CCCGGG CCC^GGG
SmlI 1732 CTTAAG C^T[CT][GA]AG
SphI 1166 GCATGC GCATG^C
SspBI 508 TGTACA T^GTACA
XmaCI 7 CCCGGG C^CCGGG
XmaI 7 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AspHI 1416 GTGCAC G[AT]GC[AT]^C
1530 GTGCTC
AvaII 1370 GGTCC G^G[AT]CC
1446 GGACC
BalI 1422 TGGCCA TGG^CCA
1929 TGGCCA
BamHI 331 GGATCC G^GATCC
1737 GGATCC
BbsI 562 GAAGAC GAAGAC(2/6)
1714 GAAGAC
BciVI 1609 GGATAC GTATCC(6/5)
2143 GGATAC
BcnI 7 CCCGG CC^[GC]GG
8 CCGGG
BpmI 1939 CTCCAG CTGGAG(16/14)
1685 CTGGAG
BpuAI 562 GAAGAC GAAGAC(2/6)
1714 GAAGAC
BsaBI 850 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2242 GATCCCAATC
BsiHKAI 1416 GTGCAC G[AT]GC[AT]^C
1530 GTGCTC
BspHI 1197 TCATGA T^CATGA
1566 TCATGA
EaeI 1422 TGGCCA [CT]^GGCC[GA]
1929 TGGCCA
HapII 8 CCGG C^CGG
1384 CCGG
HincII 77 GTCAAC GT[CT]^[GA]AC
697 GTCAAC
HindII 77 GTCAAC GT[CT]^[GA]AC
697 GTCAAC
HpaII 8 CCGG C^CGG
1384 CCGG
MamI 850 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2242 GATCCCAATC
MluNI 1422 TGGCCA TGG^CCA
1929 TGGCCA
MscI 1422 TGGCCA TGG^CCA
1929 TGGCCA
MspI 8 CCGG C^CGG
1384 CCGG
MwoI 1966 GCACCTGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2178 GCCAATCCTGC
NciI 7 CCCGG CC^[GC]GG
8 CCGGG
RcaI 1197 TCATGA T^CATGA
1566 TCATGA
Tsp45I 730 GTGAC ^GT[GC]AC
1247 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
AocI ApoI BanI BbvI BmyI
BsaJI BsgI BsiYI BslI BsmI
Bsp1286I BspLU11I BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII Eco81I EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp509I TspRI XhoII