Restriction map for
pF1KB8998
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BseAI BsgI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BssHII Bst1107I BstAPI BstBI
BstZ17I CelII ClaI CpoI DraI
DraII DraIII DrdI Eam1105I Eco47III
EcoO109I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TaqI Tru9I Tth111I
TthHB8I XbaI XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AlwI 596 GGATC GGATC(4/5)
AlwNI 640 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 921 CCTGAGG CC^T[ACGT]AGG
Asp700I 849 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 962 CCCGGG C^[CT]CG[GA]G
BciVI 872 GTATCC GTATCC(6/5)
BsaWI 329 TCCGGT [AT]^CCGG[AT]
BseRI 15 CTCCTC GAGGAG(10/8)
BsiEI 149 CGGCCG CG[GA][CT]^CG
BsmBI 1009 CGTCTC CGTCTC(1/5)
BsoBI 962 CCCGGG C^[CT]CG[GA]G
Bsp1407I 23 TGTACA T^GTACA
BsrFI 349 ACCGGC [GA]^CCGG[CT]
BsrGI 23 TGTACA T^GTACA
BssSI 354 CACGAG CACGAG(-5/-1)
BstEII 345 GGTGACC G^GT[ACGT]ACC
BstPI 345 GGTGACC G^GT[ACGT]ACC
BstYI 596 GGATCT [GA]^GATC[CT]
Bsu36I 921 CCTGAGG CC^T[ACGT]AGG
Cfr10I 349 ACCGGC [GA]^CCGG[CT]
DsaI 222 CCACGG C^C[GA][CT]GG
EagI 149 CGGCCG C^GGCCG
EarI 232 CTCTTC CTCTTC(1/4)
EclXI 149 CGGCCG C^GGCCG
Eco52I 149 CGGCCG C^GGCCG
Eco81I 921 CCTGAGG CC^T[ACGT]AGG
EcoNI 1209 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 345 GGTGACC G^GT[ACGT]ACC
Ksp632I 232 CTCTTC CTCTTC(1/4)
MflI 596 GGATCT [GA]^GATC[CT]
PflMI 377 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmaI 962 CCCGGG CCC^GGG
SmlI 1168 CTCAAG C^T[CT][GA]AG
SspBI 23 TGTACA T^GTACA
TfiI 849 GAATC G^A[AT]TC
Tsp509I 838 AATT ^AATT
Van91I 377 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 596 GGATCT [GA]^GATC[CT]
XmaCI 962 CCCGGG C^CCGGG
XmaI 962 CCCGGG C^CCGGG
XmnI 849 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccII 611 CGCG CG^CG
877 CGCG
BanI 561 GGTGCC G^G[CT][GA]CC
952 GGCACC
BfaI 421 CTAG C^TAG
1198 CTAG
BsmFI 514 GTCCC GGGAC(10/14)
1119 GTCCC
BspMI 107 ACCTGC ACCTGC(4/8)
996 GCAGGT
BstUI 611 CGCG CG^CG
877 CGCG
CfoI 212 GCGC GCG^C
610 GCGC
DpnII 597 GATC ^GATC
903 GATC
HhaI 212 GCGC GCG^C
610 GCGC
HinP1I 212 GCGC G^CGC
610 GCGC
MaeI 421 CTAG C^TAG
1198 CTAG
MboI 597 GATC ^GATC
903 GATC
MspA1I 619 CAGCTG C[AC]G^C[GT]G
947 CAGCTG
MvnI 611 CGCG CG^CG
877 CGCG
NdeII 597 GATC ^GATC
903 GATC
PleI 411 GACTC GAGTC(4/5)
1117 GAGTC
PvuII 619 CAGCTG CAG^CTG
947 CAGCTG
Sau3AI 597 GATC ^GATC
903 GATC
SfaNI 320 GCATC GCATC(5/9)
368 GCATC
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BalI BanII BbvI BcnI BmyI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrBI BsrDI BsrI BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeII MaeIII MboII
MluNI MnlI MscI MslI MspI
MvaI MwoI NciI NlaIII NlaIV
PstI RsaI SacI Sau96I ScrFI
SfcI TseI Tsp45I TspRI XcmI