Restriction map for
pF1KE0321
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# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AspI AvrII BanI BbeI
BbvCI BcgI BclI BlnI BlpI
Bpu1102I BsaBI BsiWI Bsp1407I BspDI
BspHI BsrGI BssHII BssSI Bst1107I
BstAPI BstUI BstZ17I CelII ClaI
CpoI DraI DrdI Eam1105I EcoNI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfoI SgrAI SnaBI SphI SspBI
SspI SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 2213 GTCTAC GT^[AC][GT]AC
AccIII 525 TCCGGA T^CCGGA
AcyI 114 GGCGTC G[GA]^CG[CT]C
AflII 738 CTTAAG C^TTAAG
Alw44I 1012 GTGCAC G^TGCAC
Aor51HI 518 AGCGCT AGC^GCT
ApaLI 1012 GTGCAC G^TGCAC
Asp700I 358 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 329 TGCGCA TGC^GCA
BamHI 2467 GGATCC G^GATCC
BbsI 781 GTCTTC GAAGAC(2/6)
BciVI 558 GGATAC GTATCC(6/5)
BfrI 738 CTTAAG C^TTAAG
BglII 615 AGATCT A^GATCT
BpuAI 781 GTCTTC GAAGAC(2/6)
BsaHI 114 GGCGTC G[GA]^CG[CT]C
BsaWI 525 TCCGGA [AT]^CCGG[AT]
BseAI 525 TCCGGA T^CCGGA
BseRI 2190 CTCCTC GAGGAG(10/8)
BsgI 964 CTGCAC GTGCAG(16/14)
BsiEI 1494 CGGCCG CG[GA][CT]^CG
BspEI 525 TCCGGA T^CCGGA
BspLU11I 1785 ACATGT A^CATGT
BsrBI 2517 CCGCTC CCGCTC(-3/-3)
BstBI 1850 TTCGAA TT^CGAA
BstEII 2058 GGTCACC G^GT[ACGT]ACC
BstPI 2058 GGTCACC G^GT[ACGT]ACC
BstXI 1596 CCATGACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 1494 CGGCCG C^GGCCG
EclXI 1494 CGGCCG C^GGCCG
Eco47III 518 AGCGCT AGC^GCT
Eco52I 1494 CGGCCG C^GGCCG
EcoO65I 2058 GGTCACC G^GT[ACGT]ACC
FspI 329 TGCGCA TGC^GCA
HaeII 518 AGCGCT [GA]GCGC^[CT]
Hin1I 114 GGCGTC G[GA]^CG[CT]C
HincII 1831 GTCAAC GT[CT]^[GA]AC
HindII 1831 GTCAAC GT[CT]^[GA]AC
MroI 525 TCCGGA T^CCGGA
MspA1I 1139 CAGCTG C[AC]G^C[GT]G
NaeI 1629 GCCGGC GCC^GGC
NcoI 639 CCATGG C^CATGG
NgoMIV 1629 GCCGGC G^CCGGC
NspV 1850 TTCGAA TT^CGAA
PpuMI 2589 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 1139 CAGCTG CAG^CTG
SapI 2408 GCTCTTC GCTCTTC(1/4)
SfiI 2504 GGCCCCCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1850 TTCGAA TT^CGAA
SpeI 2173 ACTAGT A^CTAGT
XbaI 669 TCTAGA T^CTAGA
XmnI 358 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 36 enzymes) #
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AflIII 164 ACGTGT A^C[GA][CT]GT
1785 ACATGT
ApaI 827 GGGCCC GGGCC^C
2076 GGGCCC
BalI 1473 TGGCCA TGG^CCA
1685 TGGCCA
BbrPI 61 CACGTG CAC^GTG
917 CACGTG
BsaAI 61 CACGTG [CT]AC^GT[GA]
917 CACGTG
BsaI 368 GAGACC GGTCTC(1/5)
1460 GAGACC
BsmBI 394 GAGACG CGTCTC(1/5)
2109 GAGACG
BsmI 245 GAATGC GAATGC(1/-1)
362 GCATTC
BspMI 1295 ACCTGC ACCTGC(4/8)
1668 GCAGGT
BsrDI 135 CATTGC GCAATG(2/0)
1689 CATTGC
BstYI 615 AGATCT [GA]^GATC[CT]
2467 GGATCC
DraIII 1006 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
2065 CACCGGGTG
EcoRI 1401 GAATTC G^AATTC
1986 GAATTC
EcoT14I 639 CCATGG C^C[AT][AT]GG
2594 CCTTGG
HphI 2060 TCACC GGTGA(8/7)
2380 TCACC
MflI 615 AGATCT [GA]^GATC[CT]
2467 GGATCC
MluNI 1473 TGGCCA TGG^CCA
1685 TGGCCA
MscI 1473 TGGCCA TGG^CCA
1685 TGGCCA
MseI 739 TTAA T^TAA
857 TTAA
PflMI 161 CCAACGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
309 CCAGGGCATGG
PmaCI 61 CACGTG CAC^GTG
917 CACGTG
PmlI 61 CACGTG CAC^GTG
917 CACGTG
PstI 2021 CTGCAG CTGCA^G
2141 CTGCAG
SacI 580 GAGCTC GAGCT^C
2321 GAGCTC
SbfI 2020 CCTGCAGG CCTGCA^GG
2140 CCTGCAGG
SmaI 824 CCCGGG CCC^GGG
2651 CCCGGG
SmlI 738 CTTAAG C^T[CT][GA]AG
1905 CTTGAG
Sse8387I 2020 CCTGCAGG CCTGCA^GG
2140 CCTGCAGG
StuI 1803 AGGCCT AGG^CCT
2034 AGGCCT
StyI 639 CCATGG C^C[AT][AT]GG
2594 CCTTGG
Tru9I 739 TTAA T^TAA
857 TTAA
Van91I 161 CCAACGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
309 CCAGGGCATGG
XcmI 1643 CCAGATACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1688 CCATTGCAGCCCTGG
XhoII 615 AGATCT [GA]^GATC[CT]
2467 GGATCC
XmaCI 824 CCCGGG C^CCGGG
2651 CCCGGG
XmaI 824 CCCGGG C^CCGGG
2651 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I