Restriction map for
pF1KE0436
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccII AccIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanII BbrPI BbvCI
BcgI BciVI BclI BglI BglII
BlnI BpmI BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiWI BsmAI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstEII BstF5I BstPI
BstUI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FokI
FseI FspI HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MspA1I MunI MvnI NaeI NcoI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 169 GTCTAC GT^[AC][GT]AC
AclI 848 AACGTT AA^CGTT
AcyI 72 GGCGCC G[GA]^CG[CT]C
AflII 981 CTTAAG C^TTAAG
AlwI 898 GATCC GGATC(4/5)
Aor51HI 144 AGCGCT AGC^GCT
AvaII 213 GGTCC G^G[AT]CC
BalI 314 TGGCCA TGG^CCA
BanI 72 GGCGCC G^G[CT][GA]CC
BbeI 72 GGCGCC GGCGC^C
BbsI 957 GAAGAC GAAGAC(2/6)
BcnI 209 CCGGG CC^[GC]GG
BfrI 981 CTTAAG C^TTAAG
BlpI 400 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 400 GCTCAGC GC^T[ACGT]AGC
BpuAI 957 GAAGAC GAAGAC(2/6)
BsaHI 72 GGCGCC G[GA]^CG[CT]C
BsiEI 153 CGACCG CG[GA][CT]^CG
BsmI 327 GAATGC GAATGC(1/-1)
BspMI 41 ACCTGC ACCTGC(4/8)
BsrFI 155 ACCGGC [GA]^CCGG[CT]
BsrI 293 ACTGG ACTGG(1/-1)
BstAPI 568 GCATTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 853 TTCGAA TT^CGAA
BstYI 897 AGATCC [GA]^GATC[CT]
CelII 400 GCTCAGC GC^T[ACGT]AGC
Cfr10I 155 ACCGGC [GA]^CCGG[CT]
Cfr13I 213 GGTCC G^G[ACGT]CC
DdeI 401 CTCAG C^T[ACGT]AG
EaeI 314 TGGCCA [CT]^GGCC[GA]
Eco47III 144 AGCGCT AGC^GCT
Hin1I 72 GGCGCC G[GA]^CG[CT]C
HincII 289 GTTGAC GT[CT]^[GA]AC
HindII 289 GTTGAC GT[CT]^[GA]AC
KasI 72 GGCGCC G^GCGCC
MflI 897 AGATCC [GA]^GATC[CT]
MluNI 314 TGGCCA TGG^CCA
MscI 314 TGGCCA TGG^CCA
NarI 72 GGCGCC GG^CGCC
NciI 209 CCGGG CC^[GC]GG
NdeI 826 CATATG CA^TATG
NspV 853 TTCGAA TT^CGAA
Psp1406I 848 AACGTT AA^CGTT
PstI 995 CTGCAG CTGCA^G
SapI 186 GCTCTTC GCTCTTC(1/4)
Sau96I 213 GGTCC G^G[ACGT]CC
SfcI 995 CTGCAG C^T[GA][CT]AG
SfoI 72 GGCGCC GGC^GCC
SfuI 853 TTCGAA TT^CGAA
SmlI 981 CTTAAG C^T[CT][GA]AG
SphI 1001 GCATGC GCATG^C
TfiI 413 GAATC G^A[AT]TC
TspRI 429 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 77 CCACCTACGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 897 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 27 GTAC GT^AC
521 GTAC
BfaI 334 CTAG C^TAG
560 CTAG
BsmFI 9 GTCCC GGGAC(10/14)
808 GTCCC
BspLU11I 339 ACATGT A^CATGT
603 ACATGT
EarI 187 CTCTTC CTCTTC(1/4)
722 GAAGAG
FauI 127 CCCGC CCCGC(4/6)
131 CCCGC
HaeII 72 GGCGCC [GA]GCGC^[CT]
144 AGCGCT
HapII 156 CCGG C^CGG
209 CCGG
HpaII 156 CCGG C^CGG
209 CCGG
HphI 730 TCACC GGTGA(8/7)
892 TCACC
Ksp632I 187 CTCTTC CTCTTC(1/4)
722 GAAGAG
MaeI 334 CTAG C^TAG
560 CTAG
MnlI 410 GAGG CCTC(7/6)
713 GAGG
MslI 78 CACCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1002 CATGCTTATG
MspI 156 CCGG C^CGG
209 CCGG
PleI 727 GAGTC GAGTC(4/5)
889 GAGTC
RsaI 27 GTAC GT^AC
521 GTAC
Tsp45I 729 GTCAC ^GT[GC]AC
891 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AflIII AluI ApoI
AspHI BbvI BmyI BsaAI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BssKI
BstNI Cac8I CfoI DpnII EcoRII
Fnu4HI HaeIII HgaI HhaI HinP1I
HinfI ItaI MaeII MaeIII MboI
MboII MseI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI Sau3AI
ScrFI SfaNI TaqI Tru9I TseI
Tsp509I TthHB8I Van91I