Restriction map for
pF1KE0435
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI Eam1105I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MaeII MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tsp45I Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 639 GGCGTC G[GA]^CG[CT]C
Aor51HI 761 AGCGCT AGC^GCT
AspHI 661 GTGCTC G[AT]GC[AT]^C
AvaII 193 GGACC G^G[AT]CC
BanI 670 GGCACC G^G[CT][GA]CC
BbsI 64 GTCTTC GAAGAC(2/6)
BpmI 765 CTCCAG CTGGAG(16/14)
BpuAI 64 GTCTTC GAAGAC(2/6)
BsaHI 639 GGCGTC G[GA]^CG[CT]C
BseRI 455 CTCCTC GAGGAG(10/8)
BsiEI 8 CGGCCG CG[GA][CT]^CG
BsiHKAI 661 GTGCTC G[AT]GC[AT]^C
BsmFI 147 GTCCC GGGAC(10/14)
BspMI 587 ACCTGC ACCTGC(4/8)
BsrDI 33 CATTGC GCAATG(2/0)
BsrI 290 CCAGT ACTGG(1/-1)
BstF5I 561 CATCC GGATG(2/0)
DdeI 684 CTTAG C^T[ACGT]AG
DpnII 216 GATC ^GATC
EaeI 8 CGGCCG [CT]^GGCC[GA]
EagI 8 CGGCCG C^GGCCG
EarI 303 GAAGAG CTCTTC(1/4)
EclXI 8 CGGCCG C^GGCCG
Eco47III 761 AGCGCT AGC^GCT
Eco52I 8 CGGCCG C^GGCCG
EcoT14I 419 CCTTGG C^C[AT][AT]GG
FauI 691 GCGGG CCCGC(4/6)
FokI 561 CATCC GGATG(9/13)
HaeII 761 AGCGCT [GA]GCGC^[CT]
HapII 110 CCGG C^CGG
Hin1I 639 GGCGTC G[GA]^CG[CT]C
HpaII 110 CCGG C^CGG
Ksp632I 303 GAAGAG CTCTTC(1/4)
MboI 216 GATC ^GATC
MseI 280 TTAA T^TAA
MspA1I 699 CAGCTG C[AC]G^C[GT]G
MspI 110 CCGG C^CGG
NdeII 216 GATC ^GATC
NspI 507 GCATGT [GA]CATG^[CT]
PflMI 585 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 486 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 699 CAGCTG CAG^CTG
Sau3AI 216 GATC ^GATC
SfaNI 560 GCATC GCATC(5/9)
StyI 419 CCTTGG C^C[AT][AT]GG
Tru9I 280 TTAA T^TAA
Tsp509I 461 AATT ^AATT
Van91I 585 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AciI 7 GCGG CCGC(-3/-1)
691 GCGG
BmyI 661 GTGCTC G[AGT]GC[ACT]^C
669 GGGCAC
BsiYI 149 CCCTTGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
585 CCACCTGCTGG
BslI 149 CCCTTGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
585 CCACCTGCTGG
BsmI 440 GCATTC GAATGC(1/-1)
679 GCATTC
Bsp1286I 661 GTGCTC G[AGT]GC[ACT]^C
669 GGGCAC
BspHI 377 TCATGA T^CATGA
482 TCATGA
HgaI 486 GACGC GACGC(5/10)
640 GCGTC
HphI 319 GGTGA GGTGA(8/7)
68 TCACC
NlaIV 423 GGGGCC GG[ACGT]^[ACGT]CC
670 GGCACC
PleI 415 GACTC GAGTC(4/5)
170 GAGTC
RcaI 377 TCATGA T^CATGA
482 TCATGA
TfiI 374 GAATC G^A[AT]TC
451 GATTC
TspRI 291 CAGTG CA[GC]TG[ACGT][ACGT]^
434 CAGTG
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI BbvI BsaJI BsmAI BssKI
BstNI Cac8I CfoI Cfr13I EcoRII
Fnu4HI HaeIII HhaI HinP1I HinfI
ItaI MboII MnlI MvaI MwoI
NlaIII Sau96I ScrFI TseI