Restriction map for
pF1KE0482
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BbeI BbrPI BbsI BcgI
BciVI BclI BfaI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstPI
BstUI Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI Hin1I HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AccI 856 GTATAC GT^[AC][GT]AC
ApaI 698 GGGCCC GGGCC^C
AvaI 776 CCCGGG C^[CT]CG[GA]G
AvaII 324 GGTCC G^G[AT]CC
BamHI 800 GGATCC G^GATCC
BglII 959 AGATCT A^GATCT
BpmI 931 CTGGAG CTGGAG(16/14)
BsoBI 776 CCCGGG C^[CT]CG[GA]G
Bst1107I 856 GTATAC GTA^TAC
BstXI 497 CCAGCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 856 GTATAC GTA^TAC
CfoI 371 GCGC GCG^C
DraII 697 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 697 AGGGCCC [GA]G^G[ACGT]CC[CT]
HaeII 370 GGCGCT [GA]GCGC^[CT]
HhaI 371 GCGC GCG^C
HinP1I 371 GCGC G^CGC
HincII 847 GTCAAC GT[CT]^[GA]AC
HindII 847 GTCAAC GT[CT]^[GA]AC
HphI 491 TCACC GGTGA(8/7)
MaeII 350 ACGT A^CGT
MslI 871 CACTTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 182 CATATG CA^TATG
PstI 446 CTGCAG CTGCA^G
SacI 949 GAGCTC GAGCT^C
SfcI 446 CTGCAG C^T[GA][CT]AG
SmaI 776 CCCGGG CCC^GGG
XmaCI 776 CCCGGG C^CCGGG
XmaI 776 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 121 GCGG CCGC(-3/-1)
915 GCGG
Asp700I 105 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
289 GAAAAGCTTC
AspHI 508 GAGCAC G[AT]GC[AT]^C
949 GAGCTC
BanI 411 GGCACC G^G[CT][GA]CC
769 GGCACC
BbvCI 901 GCTGAGG CCTCAGC(-5/-2)
909 GCTGAGG
BsgI 336 CTGCAC GTGCAG(16/14)
208 GTGCAG
BsiHKAI 508 GAGCAC G[AT]GC[AT]^C
949 GAGCTC
BsmFI 427 GGGAC GGGAC(10/14)
390 GTCCC
BstF5I 138 GGATG GGATG(2/0)
840 GGATG
FokI 138 GGATG GGATG(9/13)
840 GGATG
HgaI 472 GCGTC GACGC(5/10)
969 GCGTC
HindIII 172 AAGCTT A^AGCTT
292 AAGCTT
MseI 584 TTAA T^TAA
629 TTAA
MspA1I 449 CAGCTG C[AC]G^C[GT]G
731 CAGCTG
PvuII 449 CAGCTG CAG^CTG
731 CAGCTG
Tru9I 584 TTAA T^TAA
629 TTAA
Tsp509I 128 AATT ^AATT
634 AATT
XcmI 341 CCATCTCCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
494 CCACCAGCATCGTGG
XmnI 105 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
289 GAAAAGCTTC
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AluI AlwI AlwNI BanII BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstAPI
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI HaeIII
HapII HinfI HpaII ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI TfiI TseI Tsp45I
TspRI XhoII