Restriction map for
pF1KB3853
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AhdI AocI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BanI BbeI BbrPI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BseAI BsiWI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI ClaI
CpoI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI HphI
KasI KpnI Ksp632I KspI MaeI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 202 GTCGAC GT^[AC][GT]AC
AfaI 50 GTAC GT^AC
AgeI 198 ACCGGT A^CCGGT
Alw44I 315 GTGCAC G^TGCAC
ApaI 373 GGGCCC GGGCC^C
ApaLI 315 GTGCAC G^TGCAC
AspHI 315 GTGCAC G[AT]GC[AT]^C
AvaII 40 GGACC G^G[AT]CC
BamHI 160 GGATCC G^GATCC
BanII 373 GGGCCC G[GA]GC[CT]^C
BbvCI 150 CCTCAGC CCTCAGC(-5/-2)
BcnI 582 CCGGG CC^[GC]GG
BsaBI 327 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 198 ACCGGT [AT]^CCGG[AT]
BsgI 253 GTGCAG GTGCAG(16/14)
BsiEI 200 CGGTCG CG[GA][CT]^CG
BsiHKAI 315 GTGCAC G[AT]GC[AT]^C
BsmAI 529 GAGAC GTCTC(1/5)
BsmBI 529 GAGACG CGTCTC(1/5)
BsrDI 240 CATTGC GCAATG(2/0)
BsrFI 198 ACCGGT [GA]^CCGG[CT]
BstF5I 534 GGATG GGATG(2/0)
BstYI 160 GGATCC [GA]^GATC[CT]
Cfr10I 198 ACCGGT [GA]^CCGG[CT]
DraI 451 TTTAAA TTT^AAA
DraII 372 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 372 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 436 GAATTC G^AATTC
FokI 534 GGATG GGATG(9/13)
HindIII 95 AAGCTT A^AGCTT
MamI 327 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 160 GGATCC [GA]^GATC[CT]
MseI 452 TTAA T^TAA
NciI 582 CCGGG CC^[GC]GG
NspI 564 GCATGT [GA]CATG^[CT]
PinAI 198 ACCGGT A^CCGGT
PstI 293 CTGCAG CTGCA^G
RsaI 50 GTAC GT^AC
SalI 202 GTCGAC G^TCGAC
SfaNI 332 GCATC GCATC(5/9)
SfcI 293 CTGCAG C^T[GA][CT]AG
SmlI 92 CTCAAG C^T[CT][GA]AG
TaqI 203 TCGA T^CGA
TfiI 543 GATTC G^A[AT]TC
Tru9I 452 TTAA T^TAA
TthHB8I 203 TCGA T^CGA
XcmI 267 CCATATCAGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 160 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 121 GAATTT [GA]^AATT[CT]
436 GAATTC
AlwI 161 GATCC GGATC(4/5)
160 GGATC
AlwNI 296 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
364 CAGTGCCTG
ApoI 121 GAATTT [GA]^AATT[CT]
436 GAATTC
BbvI 358 GCAGC GCAGC(8/12)
472 GCTGC
BmyI 315 GTGCAC G[AGT]GC[ACT]^C
373 GGGCCC
BpmI 554 CTGGAG CTGGAG(16/14)
614 CTGGAG
BseRI 148 CTCCTC GAGGAG(10/8)
589 GAGGAG
Bsp1286I 315 GTGCAC G[AGT]GC[ACT]^C
373 GGGCCC
BsrI 173 ACTGG ACTGG(1/-1)
363 CCAGT
DdeI 151 CTCAG C^T[ACGT]AG
235 CTCAG
DpnII 161 GATC ^GATC
327 GATC
DsaI 248 CCATGG C^C[GA][CT]GG
308 CCATGG
EcoT14I 248 CCATGG C^C[AT][AT]GG
308 CCATGG
Fnu4HI 358 GCAGC GC^[ACGT]GC
472 GCTGC
HapII 199 CCGG C^CGG
582 CCGG
HincII 202 GTCGAC GT[CT]^[GA]AC
577 GTTGAC
HindII 202 GTCGAC GT[CT]^[GA]AC
577 GTTGAC
HpaII 199 CCGG C^CGG
582 CCGG
ItaI 358 GCAGC GC^[ACGT]GC
472 GCTGC
MaeII 444 ACGT A^CGT
575 ACGT
MboI 161 GATC ^GATC
327 GATC
MslI 63 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
498 CATGGAGGTG
MspA1I 116 CAGCGG C[AC]G^C[GT]G
273 CAGCGG
MspI 199 CCGG C^CGG
582 CCGG
NcoI 248 CCATGG C^CATGG
308 CCATGG
NdeII 161 GATC ^GATC
327 GATC
PflMI 167 CCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
363 CCAGTGCCTGG
Sau3AI 161 GATC ^GATC
327 GATC
StyI 248 CCATGG C^C[AT][AT]GG
308 CCATGG
TseI 358 GCAGC G^C[AT]GC
472 GCTGC
Tsp509I 122 AATT ^AATT
437 AATT
Van91I 167 CCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
363 CCAGTGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI BalI BbsI BpuAI
BsaJI BsiYI BslI BssKI BstNI
Cfr13I EaeI EcoRII HaeIII HinfI
MaeIII MboII MluNI MnlI MscI
MvaI MwoI NlaIII NlaIV PleI
Sau96I ScrFI Tsp45I TspRI