Restriction map for
pF1KE0337
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BbsI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BpuAI BsaBI BseAI BsiWI BsmI
Bsp1407I BspEI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI MamI MfeI
MluI MroI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Alw44I 210 GTGCAC G^TGCAC
AocI 394 CCTGAGG CC^T[ACGT]AGG
ApaLI 210 GTGCAC G^TGCAC
AspHI 210 GTGCAC G[AT]GC[AT]^C
AspI 1147 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1077 CCTCAGC CCTCAGC(-5/-2)
BcgI 877 CGAGGCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 576 GCCCCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1463 AGATCT A^GATCT
BsaAI 193 TACGTG [CT]AC^GT[GA]
BsaWI 889 TCCGGT [AT]^CCGG[AT]
BsgI 1482 CTGCAC GTGCAG(16/14)
BsiEI 240 CGGCCG CG[GA][CT]^CG
BsiHKAI 210 GTGCAC G[AT]GC[AT]^C
BsmBI 823 GAGACG CGTCTC(1/5)
BspDI 431 ATCGAT AT^CGAT
BspHI 1272 TCATGA T^CATGA
BsrDI 1507 CATTGC GCAATG(2/0)
BssSI 988 CACGAG CACGAG(-5/-1)
BstAPI 1234 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 318 GGTGACC G^GT[ACGT]ACC
BstPI 318 GGTGACC G^GT[ACGT]ACC
Bsu36I 394 CCTGAGG CC^T[ACGT]AGG
ClaI 431 ATCGAT AT^CGAT
EagI 240 CGGCCG C^GGCCG
EclXI 240 CGGCCG C^GGCCG
Eco52I 240 CGGCCG C^GGCCG
Eco81I 394 CCTGAGG CC^T[ACGT]AGG
EcoO65I 318 GGTGACC G^GT[ACGT]ACC
HaeII 644 AGCGCC [GA]GCGC^[CT]
HinfI 751 GACTC G^A[ACGT]TC
MaeII 194 ACGT A^CGT
NcoI 5 CCATGG C^CATGG
NspI 263 GCATGT [GA]CATG^[CT]
PflFI 1147 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 751 GACTC GAGTC(4/5)
PshAI 825 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1272 TCATGA T^CATGA
SmlI 1390 CTCAAG C^T[CT][GA]AG
TaqI 432 TCGA T^CGA
Tth111I 1147 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 432 TCGA T^CGA
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccII 17 CGCG CG^CG
736 CGCG
AcyI 274 GGCGTC G[GA]^CG[CT]C
637 GACGCC
AlwI 1516 GATCC GGATC(4/5)
651 GGATC
ApaI 465 GGGCCC GGGCC^C
1040 GGGCCC
BpmI 1254 CTCCAG CTGGAG(16/14)
310 CTGGAG
BsaHI 274 GGCGTC G[GA]^CG[CT]C
637 GACGCC
BsaI 859 GAGACC GGTCTC(1/5)
892 GGTCTC
BseRI 25 CTCCTC GAGGAG(10/8)
313 GAGGAG
BsrI 1177 ACTGG ACTGG(1/-1)
1398 CCAGT
BstUI 17 CGCG CG^CG
736 CGCG
BstXI 998 CCAGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1427 CCAGCCAGGTGG
BstYI 1463 AGATCT [GA]^GATC[CT]
1515 AGATCC
CfoI 571 GCGC GCG^C
645 GCGC
EarI 1123 CTCTTC CTCTTC(1/4)
820 GAAGAG
EcoNI 309 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1296 CCTGTCCAAGG
HhaI 571 GCGC GCG^C
645 GCGC
Hin1I 274 GGCGTC G[GA]^CG[CT]C
637 GACGCC
HinP1I 571 GCGC G^CGC
645 GCGC
Ksp632I 1123 CTCTTC CTCTTC(1/4)
820 GAAGAG
KspI 16 CCGCGG CCGC^GG
735 CCGCGG
MflI 1463 AGATCT [GA]^GATC[CT]
1515 AGATCC
MslI 357 CACAGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
864 CATGCGTATG
MvnI 17 CGCG CG^CG
736 CGCG
PflMI 525 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
987 CCACGAGCTGG
SacII 16 CCGCGG CCGC^GG
735 CCGCGG
SfaNI 769 GATGC GCATC(5/9)
443 GCATC
SmaI 293 CCCGGG CCC^GGG
1439 CCCGGG
Van91I 525 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
987 CCACGAGCTGG
XhoII 1463 AGATCT [GA]^GATC[CT]
1515 AGATCC
XmaCI 293 CCCGGG C^CCGGG
1439 CCCGGG
XmaI 293 CCCGGG C^CCGGG
1439 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BsaJI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BssKI BstF5I BstNI
Cac8I Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HpaII HphI ItaI
MaeI MaeIII MboI MboII MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TseI Tsp45I TspRI XcmI