Restriction map for
pF1KE0376
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI AspEI AvaI AviII AvrII
BamHI BbeI BbvCI BcgI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BseAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BsrI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TfiI XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 197 GGTACC G^GTACC
AcsI 1357 AAATTC [GA]^AATT[CT]
AcyI 1242 GGCGTC G[GA]^CG[CT]C
Alw44I 162 GTGCAC G^TGCAC
ApaLI 162 GTGCAC G^TGCAC
ApoI 1357 AAATTC [GA]^AATT[CT]
Asp718I 197 GGTACC G^GTACC
AspI 1158 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 935 TGGCCA TGG^CCA
BbrPI 249 CACGTG CAC^GTG
BbsI 272 GTCTTC GAAGAC(2/6)
BciVI 1067 GTATCC GTATCC(6/5)
BfaI 638 CTAG C^TAG
BpmI 982 CTGGAG CTGGAG(16/14)
BpuAI 272 GTCTTC GAAGAC(2/6)
BsaHI 1242 GGCGTC G[GA]^CG[CT]C
BsaI 1460 GAGACC GGTCTC(1/5)
BsaWI 1088 TCCGGT [AT]^CCGG[AT]
BsiEI 192 CGGCCG CG[GA][CT]^CG
BsmAI 1460 GAGAC GTCTC(1/5)
BspMI 860 GCAGGT ACCTGC(4/8)
BsrBI 394 CCGCTC CCGCTC(-3/-3)
BssSI 1258 CACGAG CACGAG(-5/-1)
DraII 1630 GGGACCT [GA]G^G[ACGT]CC[CT]
EagI 192 CGGCCG C^GGCCG
EarI 1267 CTCTTC CTCTTC(1/4)
EclXI 192 CGGCCG C^GGCCG
Eco52I 192 CGGCCG C^GGCCG
EcoNI 918 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1630 GGGACCT [GA]G^G[ACGT]CC[CT]
HaeII 929 AGCGCC [GA]GCGC^[CT]
Hin1I 1242 GGCGTC G[GA]^CG[CT]C
HphI 959 TCACC GGTGA(8/7)
KpnI 197 GGTACC GGTAC^C
Ksp632I 1267 CTCTTC CTCTTC(1/4)
MaeI 638 CTAG C^TAG
MluNI 935 TGGCCA TGG^CCA
MscI 935 TGGCCA TGG^CCA
PflFI 1158 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 942 CCACACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 249 CACGTG CAC^GTG
PmlI 249 CACGTG CAC^GTG
PpuMI 1630 GGGACCT [GA]G^G[AT]CC[CT]
SmlI 1455 CTCAAG C^T[CT][GA]AG
SspI 100 AATATT AAT^ATT
TaqI 654 TCGA T^CGA
Tth111I 1158 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 654 TCGA T^CGA
Van91I 942 CCACACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AspHI 90 GAGCAC G[AT]GC[AT]^C
162 GTGCAC
AvaII 921 GGACC G^G[AT]CC
1631 GGACC
BanII 985 GAGCCC G[GA]GC[CT]^C
1129 GAGCCC
BsaAI 249 CACGTG [CT]AC^GT[GA]
805 CACGTA
BsiHKAI 90 GAGCAC G[AT]GC[AT]^C
162 GTGCAC
BsrFI 190 ACCGGC [GA]^CCGG[CT]
194 GCCGGT
BstXI 1136 CCAGCTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1272 CCACTACTGTGG
Cfr10I 190 ACCGGC [GA]^CCGG[CT]
194 GCCGGT
FauI 393 CCCGC CCCGC(4/6)
560 CCCGC
HgaI 1158 GACGC GACGC(5/10)
1243 GCGTC
MseI 442 TTAA T^TAA
1141 TTAA
PstI 307 CTGCAG CTGCA^G
1615 CTGCAG
PvuII 149 CAGCTG CAG^CTG
1006 CAGCTG
SfcI 307 CTGCAG C^T[GA][CT]AG
1615 CTGCAG
Tru9I 442 TTAA T^TAA
1141 TTAA
Tsp45I 1348 GTGAC ^GT[GC]AC
1652 GTGAC
XcmI 938 CCAACCACACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1133 CCACCAGCTTAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI Asp700I
BanI BbvI BclI BmyI BsaJI
BseRI BsgI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TseI Tsp509I TspRI XhoII
XmnI