Restriction map for
pF1KB7341
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BcnI BlnI BlpI Bpu1102I BsaAI
BsaHI BseAI BsiEI BsiWI BspDI
BspEI BsrBI BsrDI BsrFI BssHII
BssSI BstEII BstPI BstUI Bsu36I
CelII Cfr10I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoT22I FauI FseI
FspI HgaI Hin1I KasI KpnI
KspI MluI MroI MslI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SacII SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I StuI SwaI Tth111I Van91I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AseI 1929 ATTAAT AT^TAAT
AsnI 1929 ATTAAT AT^TAAT
AvaI 3318 CTCGGG C^[CT]CG[GA]G
BalI 14 TGGCCA TGG^CCA
BanII 1956 GAGCCC G[GA]GC[CT]^C
BclI 4758 TGATCA T^GATCA
BglI 16 GCCATGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 4723 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4378 GAGACC GGTCTC(1/5)
BsaWI 4816 ACCGGA [AT]^CCGG[AT]
BsmBI 4820 GAGACG CGTCTC(1/5)
BsoBI 3318 CTCGGG C^[CT]CG[GA]G
BspHI 2795 TCATGA T^CATGA
Bst1107I 5266 GTATAC GTA^TAC
BstBI 5402 TTCGAA TT^CGAA
BstZ17I 5266 GTATAC GTA^TAC
DraII 8 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 889 GACGAAATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 17 CCATGG C^C[GA][CT]GG
EaeI 14 TGGCCA [CT]^GGCC[GA]
EcoO109I 8 GGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 4758 TGATCA T^GATCA
HaeII 2186 GGCGCT [GA]GCGC^[CT]
HapII 4817 CCGG C^CGG
HpaI 4155 GTTAAC GTT^AAC
HpaII 4817 CCGG C^CGG
MamI 4723 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 14 TGGCCA TGG^CCA
MscI 14 TGGCCA TGG^CCA
MspI 4817 CCGG C^CGG
NcoI 17 CCATGG C^CATGG
NdeI 797 CATATG CA^TATG
NspV 5402 TTCGAA TT^CGAA
PpuMI 8 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1929 ATTAAT AT^TAAT
RcaI 2795 TCATGA T^CATGA
SalI 886 GTCGAC G^TCGAC
SapI 3254 GAAGAGC GCTCTTC(1/4)
SfuI 5402 TTCGAA TT^CGAA
SpeI 4281 ACTAGT A^CTAGT
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AflII 2181 CTTAAG C^TTAAG
4393 CTTAAG
AspHI 2917 GAGCAC G[AT]GC[AT]^C
3689 GAGCAC
BciVI 1352 GTATCC GTATCC(6/5)
4187 GTATCC
BfrI 2181 CTTAAG C^TTAAG
4393 CTTAAG
BseRI 2190 CTCCTC GAGGAG(10/8)
3913 GAGGAG
BsgI 5461 CTGCAC GTGCAG(16/14)
4613 GTGCAG
BsiHKAI 2917 GAGCAC G[AT]GC[AT]^C
3689 GAGCAC
BspLU11I 2613 ACATGT A^CATGT
4964 ACATGT
BstAPI 1231 GCATCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3421 GCAGTGTATGC
EcoNI 577 CCTAAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1644 CCTTCTAAAGG
EcoRV 4174 GATATC GAT^ATC
5313 GATATC
MfeI 398 CAATTG C^AATTG
2113 CAATTG
MunI 398 CAATTG C^AATTG
2113 CAATTG
NlaIV 8 GGGTCC GG[ACGT]^[ACGT]CC
586 GGGACC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AvaII BbsI BbvI BfaI BglII
BmyI BpmI BpuAI BsaJI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspMI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EarI EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HincII
HindII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII XmnI