Restriction map for
pF1KE0484
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HaeIII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HphI KasI KspI MaeI MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MslI MspA1I MunI
MvnI NarI NcoI NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I StuI StyI SwaI
TaqI Tru9I Tsp45I Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 332 GGTACC G^GTACC
AcsI 206 AAATTC [GA]^AATT[CT]
AluI 531 AGCT AG^CT
AlwI 600 GGATC GGATC(4/5)
AlwNI 376 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 206 AAATTC [GA]^AATT[CT]
Asp718I 332 GGTACC G^GTACC
BanI 332 GGTACC G^G[CT][GA]CC
BanII 385 GAGCCC G[GA]GC[CT]^C
BbsI 523 GAAGAC GAAGAC(2/6)
BcnI 633 CCGGG CC^[GC]GG
BglII 545 AGATCT A^GATCT
BmyI 385 GAGCCC G[AGT]GC[ACT]^C
BpuAI 523 GAAGAC GAAGAC(2/6)
BsgI 442 GTGCAG GTGCAG(16/14)
BsiYI 375 CCAGTTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 375 CCAGTTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 385 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 461 TGTACA T^GTACA
BspMI 515 ACCTGC ACCTGC(4/8)
BsrFI 593 GCCGGC [GA]^CCGG[CT]
BsrGI 461 TGTACA T^GTACA
BstAPI 615 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 348 GGATG GGATG(2/0)
BstXI 578 CCAACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 593 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 593 GCCGGC [GA]^CCGG[CT]
EarI 106 GAAGAG CTCTTC(1/4)
FokI 348 GGATG GGATG(9/13)
KpnI 332 GGTACC GGTAC^C
Ksp632I 106 GAAGAG CTCTTC(1/4)
MaeII 20 ACGT A^CGT
NaeI 593 GCCGGC GCC^GGC
NciI 633 CCGGG CC^[GC]GG
NdeI 40 CATATG CA^TATG
NgoMIV 593 GCCGGC G^CCGGC
PflMI 375 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 437 CTGCAG CTGCA^G
SexAI 328 ACCAGGT A^CC[AT]GGT
SfcI 437 CTGCAG C^T[GA][CT]AG
SspBI 461 TGTACA T^GTACA
SspI 164 AATATT AAT^ATT
TfiI 228 GAATC G^A[AT]TC
TspRI 440 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 375 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AhdI 239 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
502 GACCAGGAGTC
AspEI 239 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
502 GACCAGGAGTC
BpmI 361 CTCCAG CTGGAG(16/14)
382 CTGGAG
BsmAI 237 GAGAC GTCTC(1/5)
300 GAGAC
BsrI 354 ACTGG ACTGG(1/-1)
375 CCAGT
BstYI 545 AGATCT [GA]^GATC[CT]
600 GGATCT
Eam1105I 239 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
502 GACCAGGAGTC
HapII 594 CCGG C^CGG
633 CCGG
HpaII 594 CCGG C^CGG
633 CCGG
MflI 545 AGATCT [GA]^GATC[CT]
600 GGATCT
MnlI 249 CCTC CCTC(7/6)
424 CCTC
MspI 594 CCGG C^CGG
633 CCGG
MwoI 30 GCAGATAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
615 GCAGATGATGC
XhoII 545 AGATCT [GA]^GATC[CT]
600 GGATCT
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AfaI BbvI BsaJI BssKI BstNI
DdeI DpnII EcoRII Fnu4HI HinfI
ItaI MboI MboII MvaI NdeII
NlaIV PleI RsaI Sau3AI ScrFI
SfaNI TseI Tsp509I