Restriction map for
pF1KE0123
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BbeI BbrPI
BbsI BbvCI BcgI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BsrI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MroI MseI
MunI MvnI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcyI 35 GACGCC G[GA]^CG[CT]C
AlwNI 778 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 70 AGCGCT AGC^GCT
AspI 35 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 257 TGGCCA TGG^CCA
BamHI 474 GGATCC G^GATCC
BanI 805 GGCACC G^G[CT][GA]CC
BciVI 294 GGATAC GTATCC(6/5)
BsaHI 35 GACGCC G[GA]^CG[CT]C
BsiEI 52 CGGCCG CG[GA][CT]^CG
BspMI 674 ACCTGC ACCTGC(4/8)
BsrDI 261 CATTGC GCAATG(2/0)
DsaI 647 CCATGG C^C[GA][CT]GG
EagI 52 CGGCCG C^GGCCG
EclXI 52 CGGCCG C^GGCCG
Eco47III 70 AGCGCT AGC^GCT
Eco52I 52 CGGCCG C^GGCCG
EcoNI 714 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 70 AGCGCT [GA]GCGC^[CT]
HgaI 35 GACGC GACGC(5/10)
Hin1I 35 GACGCC G[GA]^CG[CT]C
HphI 172 GGTGA GGTGA(8/7)
MaeIII 708 GTCAC ^GT[ACGT]AC
MluNI 257 TGGCCA TGG^CCA
MscI 257 TGGCCA TGG^CCA
MspA1I 376 CAGCTG C[AC]G^C[GT]G
NcoI 647 CCATGG C^CATGG
PflFI 35 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 81 GAGTC GAGTC(4/5)
PpuMI 540 GGGACCC [GA]G^G[AT]CC[CT]
PstI 775 CTGCAG CTGCA^G
PvuII 376 CAGCTG CAG^CTG
ScaI 305 AGTACT AGT^ACT
SmaI 110 CCCGGG CCC^GGG
SmlI 640 CTCAAG C^T[CT][GA]AG
TaqI 485 TCGA T^CGA
Tsp45I 708 GTCAC ^GT[GC]AC
Tth111I 35 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 485 TCGA T^CGA
XmaCI 110 CCCGGG C^CCGGG
XmaI 110 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 306 GTAC GT^AC
726 GTAC
ApaI 758 GGGCCC GGGCC^C
788 GGGCCC
AspHI 571 GTGCTC G[AT]GC[AT]^C
834 GAGCAC
AvaII 198 GGACC G^G[AT]CC
541 GGACC
BpmI 104 CTCCAG CTGGAG(16/14)
715 CTGGAG
BsiHKAI 571 GTGCTC G[AT]GC[AT]^C
834 GAGCAC
BsmBI 40 CGTCTC CGTCTC(1/5)
101 CGTCTC
BsrFI 54 GCCGGC [GA]^CCGG[CT]
802 GCCGGC
BstAPI 693 GCAGGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
777 GCAGGAGCTGC
BstXI 260 CCATTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
530 CCAGCATAATGG
BstYI 474 GGATCC [GA]^GATC[CT]
731 AGATCC
CfoI 71 GCGC GCG^C
678 GCGC
Cfr10I 54 GCCGGC [GA]^CCGG[CT]
802 GCCGGC
DdeI 275 CTGAG C^T[ACGT]AG
494 CTGAG
DraII 251 AGGCCCT [GA]G^G[ACGT]CC[CT]
540 GGGACCC
DraIII 167 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
327 CACATTGTG
EaeI 52 CGGCCG [CT]^GGCC[GA]
257 TGGCCA
EarI 811 CTCTTC CTCTTC(1/4)
138 GAAGAG
EcoO109I 251 AGGCCCT [GA]G^G[ACGT]CC[CT]
540 GGGACCC
EcoT14I 647 CCATGG C^C[AT][AT]GG
659 CCTTGG
HhaI 71 GCGC GCG^C
678 GCGC
HinP1I 71 GCGC G^CGC
678 GCGC
Ksp632I 811 CTCTTC CTCTTC(1/4)
138 GAAGAG
MboII 138 GAAGA GAAGA(8/7)
812 TCTTC
MflI 474 GGATCC [GA]^GATC[CT]
731 AGATCC
MslI 261 CATTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
763 CATGACTATG
NaeI 54 GCCGGC GCC^GGC
802 GCCGGC
NgoMIV 54 GCCGGC G^CCGGC
802 GCCGGC
RsaI 306 GTAC GT^AC
726 GTAC
SfcI 423 CTACAG C^T[GA][CT]AG
775 CTGCAG
StyI 647 CCATGG C^C[AT][AT]GG
659 CCTTGG
TfiI 48 GAATC G^A[AT]TC
283 GATTC
TspRI 266 CAGTG CA[GC]TG[ACGT][ACGT]^
686 CAGTG
XhoII 474 GGATCC [GA]^GATC[CT]
731 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AlwI AvaI BanII
BbvI BcnI BmyI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DpnII EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MboI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TseI
XcmI