Restriction map for
pF1KE0143
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaLI AscI AspEI AspI
AviII AvrII BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraIII Eam1105I
EarI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI HphI Ksp632I MaeI
MamI MluI MroI NcoI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tsp45I
Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 66 enzymes) #
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Acc65I 162 GGTACC G^GTACC
AcsI 300 AAATTC [GA]^AATT[CT]
AcyI 55 GGCGCC G[GA]^CG[CT]C
AocI 751 CCTCAGG CC^T[ACGT]AGG
ApoI 300 AAATTC [GA]^AATT[CT]
AseI 790 ATTAAT AT^TAAT
AsnI 790 ATTAAT AT^TAAT
Asp700I 880 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 162 GGTACC G^GTACC
AspHI 616 GAGCTC G[AT]GC[AT]^C
AvaI 93 CCCGGG C^[CT]CG[GA]G
BamHI 37 GGATCC G^GATCC
BbeI 55 GGCGCC GGCGC^C
BglI 7 GCCGCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 244 CTGGAG CTGGAG(16/14)
BsaHI 55 GGCGCC G[GA]^CG[CT]C
BsiHKAI 616 GAGCTC G[AT]GC[AT]^C
BsmAI 704 GAGAC GTCTC(1/5)
BsmBI 704 GAGACG CGTCTC(1/5)
BsoBI 93 CCCGGG C^[CT]CG[GA]G
BspMI 165 ACCTGC ACCTGC(4/8)
BsrBI 131 GAGCGG CCGCTC(-3/-3)
BsrDI 766 GCAATG GCAATG(2/0)
BsrFI 125 GCCGGC [GA]^CCGG[CT]
BstXI 656 CCAAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 37 GGATCC [GA]^GATC[CT]
Bsu36I 751 CCTCAGG CC^T[ACGT]AGG
Cfr10I 125 GCCGGC [GA]^CCGG[CT]
DraI 895 TTTAAA TTT^AAA
DrdI 875 GACTTGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 8 CCGCGG C^C[GA][CT]GG
Eco81I 751 CCTCAGG CC^T[ACGT]AGG
HaeII 55 GGCGCC [GA]GCGC^[CT]
HgaI 706 GACGC GACGC(5/10)
Hin1I 55 GGCGCC G[GA]^CG[CT]C
HindIII 839 AAGCTT A^AGCTT
KasI 55 GGCGCC G^GCGCC
KpnI 162 GGTACC GGTAC^C
KspI 8 CCGCGG CCGC^GG
MaeII 680 ACGT A^CGT
MaeIII 817 GTAAC ^GT[ACGT]AC
MfeI 437 CAATTG C^AATTG
MflI 37 GGATCC [GA]^GATC[CT]
MslI 594 CATTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 8 CCGCGG C[AC]G^C[GT]G
MunI 437 CAATTG C^AATTG
NaeI 125 GCCGGC GCC^GGC
NarI 55 GGCGCC GG^CGCC
NgoMIV 125 GCCGGC G^CCGGC
NlaIII 46 CATG CATG^
NotI 4 GCGGCCGC GC^GGCCGC
NspI 45 GCATGC [GA]CATG^[CT]
PflMI 678 CCACGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 790 ATTAAT AT^TAAT
SacI 616 GAGCTC GAGCT^C
SacII 8 CCGCGG CCGC^GG
SfaNI 346 GATGC GCATC(5/9)
SfoI 55 GGCGCC GGC^GCC
SmaI 93 CCCGGG CCC^GGG
SphI 45 GCATGC GCATG^C
StuI 722 AGGCCT AGG^CCT
Van91I 678 CCACGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 37 GGATCC [GA]^GATC[CT]
XmaCI 93 CCCGGG C^CCGGG
XmaI 93 CCCGGG C^CCGGG
XmnI 880 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 38 GATCC GGATC(4/5)
37 GGATC
ApaI 135 GGGCCC GGGCC^C
149 GGGCCC
BalI 608 TGGCCA TGG^CCA
827 TGGCCA
BbsI 213 GAAGAC GAAGAC(2/6)
884 GTCTTC
BpuAI 213 GAAGAC GAAGAC(2/6)
884 GTCTTC
BseRI 247 GAGGAG GAGGAG(10/8)
250 GAGGAG
BsiEI 5 CGGCCG CG[GA][CT]^CG
170 CGGCCG
BsrI 480 ACTGG ACTGG(1/-1)
427 CCAGT
BstF5I 345 GGATG GGATG(2/0)
800 GGATG
DpnII 38 GATC ^GATC
144 GATC
EagI 5 CGGCCG C^GGCCG
170 CGGCCG
EclXI 5 CGGCCG C^GGCCG
170 CGGCCG
Eco52I 5 CGGCCG C^GGCCG
170 CGGCCG
FokI 345 GGATG GGATG(9/13)
800 GGATG
HinfI 455 GACTC G^A[ACGT]TC
635 GAGTC
MboI 38 GATC ^GATC
144 GATC
MluNI 608 TGGCCA TGG^CCA
827 TGGCCA
MscI 608 TGGCCA TGG^CCA
827 TGGCCA
NdeII 38 GATC ^GATC
144 GATC
PleI 455 GACTC GAGTC(4/5)
635 GAGTC
PpuMI 177 GGGTCCC [GA]G^G[AT]CC[CT]
756 GGGACCT
Sau3AI 38 GATC ^GATC
144 GATC
SfcI 198 CTACAG C^T[GA][CT]AG
714 CTACAG
TaqI 641 TCGA T^CGA
809 TCGA
TspRI 78 CAGTG CA[GC]TG[ACGT][ACGT]^
662 CAGTG
TthHB8I 641 TCGA T^CGA
809 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BanI BanII BbvI BcnI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
ItaI MboII MnlI MseI MspI
MvaI MvnI MwoI NciI NlaIV
RsaI Sau96I ScrFI StyI Tru9I
TseI Tsp509I