Restriction map for
pF1KB6370
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
Acc65I AccIII AclI AfaI AflII
AflIII AgeI AluI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BamHI BanII BbeI BbvCI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstF5I
BstXI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraI DraIII DrdI
EaeI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoRV EcoT22I FbaI
FokI FseI FspI HaeII HgaI
HhaI HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MunI NarI
NciI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TfiI
Tru9I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 127 GACGTC GACGT^C
AccI 517 GTCTAC GT^[AC][GT]AC
AccII 135 CGCG CG^CG
AcsI 436 GAATTC [GA]^AATT[CT]
AcyI 127 GACGTC G[GA]^CG[CT]C
AhdI 193 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 709 GTGCAC G^TGCAC
AlwI 613 GATCC GGATC(4/5)
ApaLI 709 GTGCAC G^TGCAC
ApoI 436 GAATTC [GA]^AATT[CT]
AspEI 193 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 709 GTGCAC G[AT]GC[AT]^C
AvaI 719 CTCGGG C^[CT]CG[GA]G
BbrPI 841 CACGTG CAC^GTG
BbsI 124 GAAGAC GAAGAC(2/6)
BcgI 711 GCACTGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 124 GAAGAC GAAGAC(2/6)
BsaHI 127 GACGTC G[GA]^CG[CT]C
BsiHKAI 709 GTGCAC G[AT]GC[AT]^C
BsmI 397 GCATTC GAATGC(1/-1)
BsoBI 719 CTCGGG C^[CT]CG[GA]G
BssSI 871 CTCGTG CACGAG(-5/-1)
BstEII 630 GGTCACC G^GT[ACGT]ACC
BstPI 630 GGTCACC G^GT[ACGT]ACC
BstUI 135 CGCG CG^CG
BstYI 612 AGATCC [GA]^GATC[CT]
DsaI 209 CCATGG C^C[GA][CT]GG
Eam1105I 193 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 543 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 630 GGTCACC G^GT[ACGT]ACC
EcoRI 436 GAATTC G^AATTC
Hin1I 127 GACGTC G[GA]^CG[CT]C
HphI 632 TCACC GGTGA(8/7)
MflI 612 AGATCC [GA]^GATC[CT]
MslI 285 CATCTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 135 CGCG CG^CG
NaeI 724 GCCGGC GCC^GGC
NcoI 209 CCATGG C^CATGG
NgoMIV 724 GCCGGC G^CCGGC
NspI 117 GCATGT [GA]CATG^[CT]
PmaCI 841 CACGTG CAC^GTG
PmlI 841 CACGTG CAC^GTG
SspI 407 AATATT AAT^ATT
Tsp45I 631 GTCAC ^GT[GC]AC
XcmI 533 CCAAGGCTTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 612 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BanI 12 GGTGCC G^G[CT][GA]CC
876 GGTGCC
BsaI 295 GAGACC GGTCTC(1/5)
338 GGTCTC
BseRI 547 GAGGAG GAGGAG(10/8)
550 GAGGAG
BsrFI 605 ACCGGC [GA]^CCGG[CT]
724 GCCGGC
Cfr10I 605 ACCGGC [GA]^CCGG[CT]
724 GCCGGC
DdeI 203 CTCAG C^T[ACGT]AG
743 CTGAG
DpnII 372 GATC ^GATC
613 GATC
HapII 606 CCGG C^CGG
725 CCGG
HpaII 606 CCGG C^CGG
725 CCGG
MboI 372 GATC ^GATC
613 GATC
MspA1I 390 CAGCGG C[AC]G^C[GT]G
526 CAGCGG
MspI 606 CCGG C^CGG
725 CCGG
NdeII 372 GATC ^GATC
613 GATC
NlaIII 118 CATG CATG^
210 CATG
PpuMI 45 GGGTCCT [GA]G^G[AT]CC[CT]
461 GGGTCCC
Sau3AI 372 GATC ^GATC
613 GATC
TaqI 374 TCGA T^CGA
470 TCGA
Tsp509I 437 AATT ^AATT
702 AATT
TthHB8I 374 TCGA T^CGA
470 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AvaII BbvI BmyI BpmI
BsaAI BsaJI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeIII
HinfI ItaI MaeII MaeIII MboII
MnlI MvaI MwoI NlaIV PleI
Sau96I ScrFI StyI TseI TspRI