Restriction map for
pF1KB6777
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# NO CUT ENZYMES ( 200 enzymes) #
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AatII Acc65I AccI AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsiYI BslI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I EcoT22I FauI FbaI Fnu4HI
FseI FspI HaeII HapII HgaI
Hin1I HindIII HpaI HpaII ItaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MslI MspA1I MspI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TseI Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 30 CGCG CG^CG
AluI 21 AGCT AG^CT
AlwI 345 GGATC GGATC(4/5)
AspHI 25 GTGCTC G[AT]GC[AT]^C
AspI 315 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BmyI 25 GTGCTC G[AGT]GC[ACT]^C
BpmI 52 CTGGAG CTGGAG(16/14)
BsaJI 11 CCGTGG C^C[ACGT][ACGT]GG
BsiHKAI 25 GTGCTC G[AT]GC[AT]^C
BsmI 210 GCATTC GAATGC(1/-1)
Bsp1286I 25 GTGCTC G[AGT]GC[ACT]^C
BsrI 236 ACTGG ACTGG(1/-1)
BssKI 51 CCTGG ^CC[ACGT]GG
BstNI 51 CCTGG CC^[AT]GG
BstUI 30 CGCG CG^CG
CfoI 31 GCGC GCG^C
DdeI 18 CTTAG C^T[ACGT]AG
DpnII 346 GATC ^GATC
DsaI 11 CCGTGG C^C[GA][CT]GG
EcoRI 265 GAATTC G^AATTC
EcoRII 51 CCTGG ^CC[AT]GG
HhaI 31 GCGC GCG^C
HinP1I 31 GCGC G^CGC
HincII 169 GTTGAC GT[CT]^[GA]AC
HindII 169 GTTGAC GT[CT]^[GA]AC
HinfI 78 GATTC G^A[ACGT]TC
HphI 269 TCACC GGTGA(8/7)
MaeII 93 ACGT A^CGT
MboI 346 GATC ^GATC
MboII 180 GAAGA GAAGA(8/7)
MnlI 55 GAGG CCTC(7/6)
MseI 338 TTAA T^TAA
MvaI 51 CCTGG CC^[AT]GG
MvnI 30 CGCG CG^CG
MwoI 22 GCTGTGCTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 346 GATC ^GATC
PflFI 315 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Sau3AI 346 GATC ^GATC
ScrFI 51 CCTGG CC^[ACGT]GG
TaqI 348 TCGA T^CGA
TfiI 78 GATTC G^A[AT]TC
Tru9I 338 TTAA T^TAA
Tth111I 315 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 348 TCGA T^CGA
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# TWO CUT ENZYMES ( 11 enzymes) #
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AcsI 120 AAATTT [GA]^AATT[CT]
265 GAATTC
AfaI 68 GTAC GT^AC
255 GTAC
ApoI 120 AAATTT [GA]^AATT[CT]
265 GAATTC
BsmAI 350 GAGAC GTCTC(1/5)
3 GTCTC
Cac8I 27 GCTCGC GC[ACGT]^[ACGT]GC
295 GCCTGC
HaeIII 15 GGCC GG^CC
49 GGCC
MaeIII 313 GTGAC ^GT[ACGT]AC
321 GTCAC
NlaIII 310 CATG CATG^
354 CATG
RsaI 68 GTAC GT^AC
255 GTAC
Tsp45I 313 GTGAC ^GT[GC]AC
321 GTCAC
TspRI 261 CACTG CA[GC]TG[ACGT][ACGT]^
276 CACTG
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# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
#########################################
BstF5I FokI Tsp509I