Restriction map for
pF1KB6132
##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI AlwI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BalI
BamHI BciVI BclI BfrI BglII
BlnI BsaBI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BsrI BssSI BstBI BstEII BstPI
BstYI Bsu36I ClaI DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MamI MflI
MluI MluNI MscI MseI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI SalI SapI
SbfI ScaI SexAI SfuI SgrAI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Van91I XbaI XcmI
XhoII XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 900 GTATAC GT^[AC][GT]AC
AccIII 1840 TCCGGA T^CCGGA
Alw44I 1156 GTGCAC G^TGCAC
ApaLI 1156 GTGCAC G^TGCAC
BbeI 323 GGCGCC GGCGC^C
BcgI 1133 GCAGTGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 232 CTAG C^TAG
BsaI 453 GGTCTC GGTCTC(1/5)
BsaWI 1840 TCCGGA [AT]^CCGG[AT]
BseAI 1840 TCCGGA T^CCGGA
BspEI 1840 TCCGGA T^CCGGA
BssHII 28 GCGCGC G^CGCGC
Bst1107I 900 GTATAC GTA^TAC
BstZ17I 900 GTATAC GTA^TAC
CpoI 623 CGGTCCG CG^G[AT]CCG
EagI 744 CGGCCG C^GGCCG
EclXI 744 CGGCCG C^GGCCG
Eco52I 744 CGGCCG C^GGCCG
EcoNI 726 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 323 GGCGCC G^GCGCC
MaeI 232 CTAG C^TAG
MfeI 1459 CAATTG C^AATTG
MroI 1840 TCCGGA T^CCGGA
MunI 1459 CAATTG C^AATTG
NarI 323 GGCGCC GG^CGCC
PaeR7I 952 CTCGAG C^TCGAG
PpuMI 997 GGGACCT [GA]G^G[AT]CC[CT]
PstI 1008 CTGCAG CTGCA^G
RsrII 623 CGGTCCG CG^G[AT]CCG
SfoI 323 GGCGCC GGC^GCC
SmlI 952 CTCGAG C^T[CT][GA]AG
SnaBI 1264 TACGTA TAC^GTA
Tsp509I 1460 AATT ^AATT
XhoI 952 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 323 GGCGCC G[GA]^CG[CT]C
694 GACGCC
AfaI 109 GTAC GT^AC
841 GTAC
AflIII 368 ACGTGT A^C[GA][CT]GT
425 ACGTGT
AlwNI 395 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
568 CAGCGCCTG
ApaI 35 GGGCCC GGGCC^C
1725 GGGCCC
BbrPI 549 CACGTG CAC^GTG
796 CACGTG
BbsI 1600 GTCTTC GAAGAC(2/6)
1699 GTCTTC
BlpI 1095 GCTCAGC GC^T[ACGT]AGC
1782 GCTGAGC
Bpu1102I 1095 GCTCAGC GC^T[ACGT]AGC
1782 GCTGAGC
BpuAI 1600 GTCTTC GAAGAC(2/6)
1699 GTCTTC
BsaHI 323 GGCGCC G[GA]^CG[CT]C
694 GACGCC
BseRI 1684 CTCCTC GAGGAG(10/8)
1844 GAGGAG
BsiEI 207 CGACCG CG[GA][CT]^CG
744 CGGCCG
BspMI 511 ACCTGC ACCTGC(4/8)
668 ACCTGC
BsrBI 210 CCGCTC CCGCTC(-3/-3)
993 GAGCGG
BsrFI 18 ACCGGC [GA]^CCGG[CT]
1816 ACCGGC
BstAPI 394 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1158 GCACGGCCTGC
CelII 1095 GCTCAGC GC^T[ACGT]AGC
1782 GCTGAGC
Cfr10I 18 ACCGGC [GA]^CCGG[CT]
1816 ACCGGC
DpnII 504 GATC ^GATC
546 GATC
Hin1I 323 GGCGCC G[GA]^CG[CT]C
694 GACGCC
KspI 40 CCGCGG CCGC^GG
1425 CCGCGG
MboI 504 GATC ^GATC
546 GATC
MboII 1601 TCTTC GAAGA(8/7)
1700 TCTTC
MslI 76 CACCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1653 CATGGCCGTG
NdeII 504 GATC ^GATC
546 GATC
PmaCI 549 CACGTG CAC^GTG
796 CACGTG
PmlI 549 CACGTG CAC^GTG
796 CACGTG
RsaI 109 GTAC GT^AC
841 GTAC
SacII 40 CCGCGG CCGC^GG
1425 CCGCGG
Sau3AI 504 GATC ^GATC
546 GATC
SfcI 720 CTACAG C^T[GA][CT]AG
1008 CTGCAG
SfiI 736 GGCCGCCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1647 GGCCGTCATGGCC
SmaI 284 CCCGGG CCC^GGG
983 CCCGGG
StuI 632 AGGCCT AGG^CCT
1274 AGGCCT
TaqI 953 TCGA T^CGA
1247 TCGA
TfiI 948 GAATC G^A[AT]TC
1249 GATTC
TthHB8I 953 TCGA T^CGA
1247 TCGA
XmaCI 284 CCCGGG C^CCGGG
983 CCCGGG
XmaI 284 CCCGGG C^CCGGG
983 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AluI AspHI AspI
AvaI AvaII BanI BanII BbvCI
BbvI BcnI BglI BmyI BpmI
BsaAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PflFI PleI PvuII SacI Sau96I
ScrFI SfaNI StyI TseI Tsp45I
TspRI Tth111I