Restriction map for
pF1KB6463
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AspEI
AspHI AvaI AviII AvrII BalI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HincII
HindII HpaI HphI KasI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 412 GGTACC G^GTACC
AccII 70 CGCG CG^CG
AcyI 3 GGCGTC G[GA]^CG[CT]C
AocI 787 CCTGAGG CC^T[ACGT]AGG
AseI 553 ATTAAT AT^TAAT
AsnI 553 ATTAAT AT^TAAT
Asp718I 412 GGTACC G^GTACC
AspI 148 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 802 GGTCC G^G[AT]CC
BamHI 54 GGATCC G^GATCC
BanI 412 GGTACC G^G[CT][GA]CC
BfaI 713 CTAG C^TAG
BglII 279 AGATCT A^GATCT
BmyI 614 GGGCAC G[AGT]GC[ACT]^C
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BseRI 772 GAGGAG GAGGAG(10/8)
BsmFI 559 GTCCC GGGAC(10/14)
Bsp1286I 614 GGGCAC G[AGT]GC[ACT]^C
BspHI 941 TCATGA T^CATGA
BsrDI 344 GCAATG GCAATG(2/0)
BstUI 70 CGCG CG^CG
BstXI 518 CCAAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 787 CCTGAGG CC^T[ACGT]AGG
Cac8I 21 GCGTGC GC[ACGT]^[ACGT]GC
CfoI 69 GCGC GCG^C
DsaI 26 CCGTGG C^C[GA][CT]GG
Eco81I 787 CCTGAGG CC^T[ACGT]AGG
FauI 610 GCGGG CCCGC(4/6)
HaeIII 529 GGCC GG^CC
HapII 52 CCGG C^CGG
HgaI 4 GCGTC GACGC(5/10)
HhaI 69 GCGC GCG^C
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HinP1I 69 GCGC G^CGC
HindIII 185 AAGCTT A^AGCTT
HinfI 548 GATTC G^A[ACGT]TC
HpaII 52 CCGG C^CGG
KpnI 412 GGTACC GGTAC^C
MaeI 713 CTAG C^TAG
MslI 844 CACAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 490 CAGCTG C[AC]G^C[GT]G
MspI 52 CCGG C^CGG
MvnI 70 CGCG CG^CG
PflFI 148 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 553 ATTAAT AT^TAAT
PvuII 490 CAGCTG CAG^CTG
RcaI 941 TCATGA T^CATGA
SspI 172 AATATT AAT^ATT
TaqI 95 TCGA T^CGA
TfiI 548 GATTC G^A[AT]TC
Tth111I 148 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 95 TCGA T^CGA
XcmI 515 CCACCAAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 413 GTAC GT^AC
585 GTAC
AlwI 55 GATCC GGATC(4/5)
54 GGATC
Asp700I 427 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
826 GAACCAGTTC
BbsI 109 GAAGAC GAAGAC(2/6)
337 GAAGAC
BpuAI 109 GAAGAC GAAGAC(2/6)
337 GAAGAC
BstYI 54 GGATCC [GA]^GATC[CT]
279 AGATCT
Cfr13I 528 GGGCC G^G[ACGT]CC
802 GGTCC
DraI 390 TTTAAA TTT^AAA
504 TTTAAA
MaeIII 125 GTGAC ^GT[ACGT]AC
868 GTGAC
MflI 54 GGATCC [GA]^GATC[CT]
279 AGATCT
PstI 232 CTGCAG CTGCA^G
266 CTGCAG
RsaI 413 GTAC GT^AC
585 GTAC
Sau96I 528 GGGCC G^G[ACGT]CC
802 GGTCC
SfcI 232 CTGCAG C^T[GA][CT]AG
266 CTGCAG
Tsp45I 125 GTGAC ^GT[GC]AC
868 GTGAC
XhoII 54 GGATCC [GA]^GATC[CT]
279 AGATCT
XmnI 427 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
826 GAACCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BsaJI BsrI BssKI BstF5I BstNI
DdeI DpnII EarI EcoRII Fnu4HI
FokI ItaI Ksp632I MboI MboII
MnlI MseI MvaI NdeII NlaIII
NlaIV Sau3AI ScrFI Tru9I TseI
Tsp509I TspRI