##################################
# NO CUT ENZYMES ( 179 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRV EcoT14I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MslI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AciI 902 GCGG CCGC(-3/-1)
AcsI 406 GAATTC [GA]^AATT[CT]
AflII 522 CTTAAG C^TTAAG
AflIII 121 ACGTGT A^C[GA][CT]GT
AlwI 429 GGATC GGATC(4/5)
ApoI 406 GAATTC [GA]^AATT[CT]
AvaII 655 GGTCC G^G[AT]CC
BfaI 818 CTAG C^TAG
BfrI 522 CTTAAG C^TTAAG
BsaI 142 GAGACC GGTCTC(1/5)
BsaJI 250 CCAGGG C^C[ACGT][ACGT]GG
BsgI 835 GTGCAG GTGCAG(16/14)
BsrDI 505 GCAATG GCAATG(2/0)
Bst1107I 235 GTATAC GTA^TAC
BstEII 904 GGTAACC G^GT[ACGT]ACC
BstPI 904 GGTAACC G^GT[ACGT]ACC
BstXI 714 CCAGAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 235 GTATAC GTA^TAC
Cfr13I 655 GGTCC G^G[ACGT]CC
DraI 597 TTTAAA TTT^AAA
EcoNI 641 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 904 GGTAACC G^GT[ACGT]ACC
EcoRI 406 GAATTC G^AATTC
EcoT22I 1 ATGCAT ATGCA^T
HaeIII 771 GGCC GG^CC
HindIII 393 AAGCTT A^AGCTT
MaeI 818 CTAG C^TAG
MaeII 121 ACGT A^CGT
MspA1I 900 CAGCGG C[AC]G^C[GT]G
MwoI 310 GCTGATGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 579 CATG CATG^
NsiI 1 ATGCAT ATGCA^T
PleI 754 GACTC GAGTC(4/5)
Sau96I 655 GGTCC G^G[ACGT]CC
SmlI 522 CTTAAG C^T[CT][GA]AG
TfiI 17 GAATC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 101 GTCTAC GT^[AC][GT]AC
235 GTATAC
BsiYI 641 CCTGGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
781 CCAGCAGGAGG
BslI 641 CCTGGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
781 CCAGCAGGAGG
BsmAI 142 GAGAC GTCTC(1/5)
840 GTCTC
BstF5I 301 CATCC GGATG(2/0)
847 CATCC
EarI 804 CTCTTC CTCTTC(1/4)
273 GAAGAG
FokI 301 CATCC GGATG(9/13)
847 CATCC
HinfI 17 GAATC G^A[ACGT]TC
754 GACTC
HphI 471 GGTGA GGTGA(8/7)
711 TCACC
Ksp632I 804 CTCTTC CTCTTC(1/4)
273 GAAGAG
MseI 523 TTAA T^TAA
598 TTAA
NlaIV 591 GGAACC GG[ACGT]^[ACGT]CC
883 GGAACC
ScaI 826 AGTACT AGT^ACT
871 AGTACT
Tru9I 523 TTAA T^TAA
598 TTAA
TspRI 27 CACTG CA[GC]TG[ACGT][ACGT]^
833 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AfaI AluI BbvI BpmI BseRI
BsrI BssKI BstNI DdeI DpnII
EcoRII Fnu4HI ItaI MaeIII MboI
MboII MnlI MvaI NdeII RsaI
Sau3AI ScrFI SfaNI TaqI TseI
Tsp45I Tsp509I TthHB8I