################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRV EcoT14I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AciI 902 GCGG CCGC(-3/-1) AcsI 406 GAATTC [GA]^AATT[CT] AflII 522 CTTAAG C^TTAAG AflIII 121 ACGTGT A^C[GA][CT]GT AlwI 429 GGATC GGATC(4/5) ApoI 406 GAATTC [GA]^AATT[CT] AvaII 655 GGTCC G^G[AT]CC BfaI 818 CTAG C^TAG BfrI 522 CTTAAG C^TTAAG BsaI 142 GAGACC GGTCTC(1/5) BsaJI 250 CCAGGG C^C[ACGT][ACGT]GG BsgI 835 GTGCAG GTGCAG(16/14) BsrDI 505 GCAATG GCAATG(2/0) Bst1107I 235 GTATAC GTA^TAC BstEII 904 GGTAACC G^GT[ACGT]ACC BstPI 904 GGTAACC G^GT[ACGT]ACC BstXI 714 CCAGAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 235 GTATAC GTA^TAC Cfr13I 655 GGTCC G^G[ACGT]CC DraI 597 TTTAAA TTT^AAA EcoNI 641 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 904 GGTAACC G^GT[ACGT]ACC EcoRI 406 GAATTC G^AATTC EcoT22I 1 ATGCAT ATGCA^T HaeIII 771 GGCC GG^CC HindIII 393 AAGCTT A^AGCTT MaeI 818 CTAG C^TAG MaeII 121 ACGT A^CGT MspA1I 900 CAGCGG C[AC]G^C[GT]G MwoI 310 GCTGATGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIII 579 CATG CATG^ NsiI 1 ATGCAT ATGCA^T PleI 754 GACTC GAGTC(4/5) Sau96I 655 GGTCC G^G[ACGT]CC SmlI 522 CTTAAG C^T[CT][GA]AG TfiI 17 GAATC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AccI 101 GTCTAC GT^[AC][GT]AC 235 GTATAC BsiYI 641 CCTGGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 781 CCAGCAGGAGG BslI 641 CCTGGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 781 CCAGCAGGAGG BsmAI 142 GAGAC GTCTC(1/5) 840 GTCTC BstF5I 301 CATCC GGATG(2/0) 847 CATCC EarI 804 CTCTTC CTCTTC(1/4) 273 GAAGAG FokI 301 CATCC GGATG(9/13) 847 CATCC HinfI 17 GAATC G^A[ACGT]TC 754 GACTC HphI 471 GGTGA GGTGA(8/7) 711 TCACC Ksp632I 804 CTCTTC CTCTTC(1/4) 273 GAAGAG MseI 523 TTAA T^TAA 598 TTAA NlaIV 591 GGAACC GG[ACGT]^[ACGT]CC 883 GGAACC ScaI 826 AGTACT AGT^ACT 871 AGTACT Tru9I 523 TTAA T^TAA 598 TTAA TspRI 27 CACTG CA[GC]TG[ACGT][ACGT]^ 833 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AfaI AluI BbvI BpmI BseRI BsrI BssKI BstNI DdeI DpnII EcoRII Fnu4HI ItaI MaeIII MboI MboII MnlI MvaI NdeII RsaI Sau3AI ScrFI SfaNI TaqI TseI Tsp45I Tsp509I TthHB8I