Restriction map for
pF1KB7707
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BclI BfrI
BglI BglII BlnI BsaBI BsaWI
BseAI BseRI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstBI BstZ17I
CfoI ClaI CpoI DraI DraIII
DrdI EarI Eco47III EcoNI EcoRI
EcoRV EcoT14I EcoT22I FbaI FspI
HaeII HhaI HinP1I HincII HindII
HpaI KasI KpnI Ksp632I MamI
MfeI MluI MluNI MroI MscI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspBI SspI StuI StyI SwaI
TfiI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 276 CGCG CG^CG
AcyI 783 GGCGTC G[GA]^CG[CT]C
AhdI 865 GACAAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1103 GGATC GGATC(4/5)
Asp700I 1016 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 865 GACAAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 81 GTGCTC G[AT]GC[AT]^C
AvaI 962 CCCGAG C^[CT]CG[GA]G
BanII 1211 GAGCCC G[GA]GC[CT]^C
BciVI 270 GGATAC GTATCC(6/5)
BlpI 829 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 829 GCTGAGC GC^T[ACGT]AGC
BsaAI 577 TACGTG [CT]AC^GT[GA]
BsaHI 783 GGCGTC G[GA]^CG[CT]C
BsgI 414 CTGCAC GTGCAG(16/14)
BsiEI 1048 CGGCCG CG[GA][CT]^CG
BsiHKAI 81 GTGCTC G[AT]GC[AT]^C
BsmBI 637 GAGACG CGTCTC(1/5)
BsoBI 962 CCCGAG C^[CT]CG[GA]G
BsrFI 1050 GCCGGC [GA]^CCGG[CT]
BssSI 1236 CACGAG CACGAG(-5/-1)
BstUI 276 CGCG CG^CG
BstYI 1103 GGATCT [GA]^GATC[CT]
CelII 829 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1050 GCCGGC [GA]^CCGG[CT]
DraII 113 GGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 1048 CGGCCG C^GGCCG
Eam1105I 865 GACAAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1048 CGGCCG C^GGCCG
Eco52I 1048 CGGCCG C^GGCCG
EcoO109I 113 GGGTCCT [GA]G^G[ACGT]CC[CT]
FauI 791 CCCGC CCCGC(4/6)
FseI 1049 GGCCGGCC GGCCGG^CC
Hin1I 783 GGCGTC G[GA]^CG[CT]C
HindIII 551 AAGCTT A^AGCTT
HinfI 52 GACTC G^A[ACGT]TC
KspI 275 CCGCGG CCGC^GG
MflI 1103 GGATCT [GA]^GATC[CT]
MseI 367 TTAA T^TAA
MvnI 276 CGCG CG^CG
NaeI 1050 GCCGGC GCC^GGC
NgoMIV 1050 GCCGGC G^CCGGC
PflMI 912 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 52 GACTC GAGTC(4/5)
PpuMI 113 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 232 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 275 CCGCGG CCGC^GG
SbfI 795 CCTGCAGG CCTGCA^GG
Sse8387I 795 CCTGCAGG CCTGCA^GG
Tru9I 367 TTAA T^TAA
Van91I 912 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 35 CCACGGAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1103 GGATCT [GA]^GATC[CT]
XmnI 1016 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 1114 GTAC GT^AC
1181 GTAC
AocI 1025 CCTTAGG CC^T[ACGT]AGG
1186 CCTCAGG
BfaI 449 CTAG C^TAG
1223 CTAG
BmyI 81 GTGCTC G[AGT]GC[ACT]^C
1211 GAGCCC
BpmI 901 CTCCAG CTGGAG(16/14)
1136 CTGGAG
Bsp1286I 81 GTGCTC G[AGT]GC[ACT]^C
1211 GAGCCC
BspMI 373 ACCTGC ACCTGC(4/8)
770 ACCTGC
BsrBI 924 CCGCTC CCGCTC(-3/-3)
320 GAGCGG
Bsu36I 1025 CCTTAGG CC^T[ACGT]AGG
1186 CCTCAGG
DpnII 721 GATC ^GATC
1104 GATC
DsaI 35 CCACGG C^C[GA][CT]GG
275 CCGCGG
Eco81I 1025 CCTTAGG CC^T[ACGT]AGG
1186 CCTCAGG
HgaI 362 GCGTC GACGC(5/10)
784 GCGTC
MaeI 449 CTAG C^TAG
1223 CTAG
MaeII 578 ACGT A^CGT
640 ACGT
MboI 721 GATC ^GATC
1104 GATC
NdeII 721 GATC ^GATC
1104 GATC
NspI 458 ACATGC [GA]CATG^[CT]
1082 GCATGT
PvuII 73 CAGCTG CAG^CTG
835 CAGCTG
RsaI 1114 GTAC GT^AC
1181 GTAC
Sau3AI 721 GATC ^GATC
1104 GATC
TaqI 240 TCGA T^CGA
606 TCGA
Tsp509I 755 AATT ^AATT
1021 AATT
TthHB8I 240 TCGA T^CGA
606 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwNI AvaII BbsI
BbvI BcnI BpuAI BsaI BsaJI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstXI Cac8I Cfr13I DdeI
EaeI EcoO65I EcoRII Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeIII MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PstI Sau96I ScrFI SfaNI
SfcI TseI Tsp45I TspRI