Restriction map for
pF1KE0121
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstYI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DpnII
DraI DraIII DrdI DsaI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRII EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HphI KasI KpnI
Ksp632I KspI MaeI MaeII MaeIII
MamI MboI MfeI MflI MluI
MroI MslI MspA1I MunI MvaI
MvnI NaeI NarI NciI NcoI
NdeI NdeII NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI ScrFI SexAI SfiI
SfoI SfuI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI TfiI Tsp45I TspRI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AfaI 93 GTAC GT^AC
AvaII 109 GGTCC G^G[AT]CC
BalI 179 TGGCCA TGG^CCA
BanI 13 GGTGCC G^G[CT][GA]CC
BanII 131 GGGCTC G[GA]GC[CT]^C
BsaJI 18 CCGAGG C^C[ACGT][ACGT]GG
BseRI 46 CTCCTC GAGGAG(10/8)
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsiYI 35 CCTACCACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 35 CCTACCACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 110 GTCCC GGGAC(10/14)
Bsp1407I 92 TGTACA T^GTACA
BsrGI 92 TGTACA T^GTACA
BstF5I 100 CATCC GGATG(2/0)
BstXI 271 CCAAAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 108 AGGTCCC [GA]G^G[ACGT]CC[CT]
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
EcoO109I 108 AGGTCCC [GA]G^G[ACGT]CC[CT]
FauI 27 GCGGG CCCGC(4/6)
FokI 100 CATCC GGATG(9/13)
HinfI 282 GAGTC G^A[ACGT]TC
MboII 125 TCTTC GAAGA(8/7)
MluNI 179 TGGCCA TGG^CCA
MscI 179 TGGCCA TGG^CCA
MseI 296 TTAA T^TAA
PleI 282 GAGTC GAGTC(4/5)
PpuMI 108 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 188 CTGCAG CTGCA^G
RsaI 93 GTAC GT^AC
SfaNI 66 GATGC GCATC(5/9)
SgrAI 9 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 92 TGTACA T^GTACA
StuI 21 AGGCCT AGG^CCT
TaqI 50 TCGA T^CGA
Tru9I 296 TTAA T^TAA
TthHB8I 50 TCGA T^CGA
XcmI 136 CCAATTATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 8 CCGC CCGC(-3/-1)
27 GCGG
AvaI 17 CCCGAG C^[CT]CG[GA]G
73 CCCGAG
BbvI 313 GCAGC GCAGC(8/12)
69 GCTGC
BmyI 14 GTGCCC G[AGT]GC[ACT]^C
131 GGGCTC
BsoBI 17 CCCGAG C^[CT]CG[GA]G
73 CCCGAG
Bsp1286I 14 GTGCCC G[AGT]GC[ACT]^C
131 GGGCTC
BspMI 186 ACCTGC ACCTGC(4/8)
106 GCAGGT
BsrFI 10 GCCGGT [GA]^CCGG[CT]
41 ACCGGC
Cac8I 23 GCCTGC GC[ACGT]^[ACGT]GC
27 GCGGGC
Cfr10I 10 GCCGGT [GA]^CCGG[CT]
41 ACCGGC
Cfr13I 29 GGGCC G^G[ACGT]CC
109 GGTCC
DdeI 199 CTTAG C^T[ACGT]AG
326 CTCAG
EaeI 5 CGGCCG [CT]^GGCC[GA]
179 TGGCCA
HapII 11 CCGG C^CGG
42 CCGG
HpaII 11 CCGG C^CGG
42 CCGG
MspI 11 CCGG C^CGG
42 CCGG
Sau96I 29 GGGCC G^G[ACGT]CC
109 GGTCC
SfcI 188 CTGCAG C^T[GA][CT]AG
230 CTACAG
TseI 69 GCTGC G^C[AT]GC
313 GCAGC
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
AluI Fnu4HI HaeIII ItaI MnlI
MwoI NlaIV Tsp509I