Restriction map for
pF1KB8871
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmFI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstF5I BstPI BstUI
BstXI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeIII MamI MfeI MflI MluI
MroI MslI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tsp45I
Tsp509I Tth111I TthHB8I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AfaI 366 GTAC GT^AC
AflII 103 CTTAAG C^TTAAG
AlwNI 280 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 69 CCTGAGG CC^T[ACGT]AGG
AspHI 142 GAGCAC G[AT]GC[AT]^C
AvaI 77 CCCGGG C^[CT]CG[GA]G
BalI 242 TGGCCA TGG^CCA
BbsI 109 GTCTTC GAAGAC(2/6)
BfrI 103 CTTAAG C^TTAAG
BglI 376 GCCCCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 109 GTCTTC GAAGAC(2/6)
BsaAI 454 TACGTG [CT]AC^GT[GA]
BsgI 274 CTGCAC GTGCAG(16/14)
BsiHKAI 142 GAGCAC G[AT]GC[AT]^C
BsmAI 466 GTCTC GTCTC(1/5)
BsmI 200 GCATTC GAATGC(1/-1)
BsoBI 77 CCCGGG C^[CT]CG[GA]G
BspMI 406 ACCTGC ACCTGC(4/8)
BstAPI 276 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 126 CCAGG CC^[AT]GG
Bsu36I 69 CCTGAGG CC^T[ACGT]AGG
CfoI 121 GCGC GCG^C
Eco81I 69 CCTGAGG CC^T[ACGT]AGG
EcoRII 126 CCAGG ^CC[AT]GG
EcoT14I 179 CCAAGG C^C[AT][AT]GG
FauI 116 GCGGG CCCGC(4/6)
HaeII 120 GGCGCT [GA]GCGC^[CT]
HgaI 359 GCGTC GACGC(5/10)
HhaI 121 GCGC GCG^C
HinP1I 121 GCGC G^CGC
HinfI 257 GACTC G^A[ACGT]TC
HphI 404 TCACC GGTGA(8/7)
MaeII 455 ACGT A^CGT
MboII 110 TCTTC GAAGA(8/7)
MluNI 242 TGGCCA TGG^CCA
MscI 242 TGGCCA TGG^CCA
MseI 104 TTAA T^TAA
MvaI 126 CCAGG CC^[AT]GG
NlaIII 24 CATG CATG^
NspI 23 ACATGC [GA]CATG^[CT]
PflMI 213 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 257 GACTC GAGTC(4/5)
PpuMI 73 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 366 GTAC GT^AC
SfaNI 206 GCATC GCATC(5/9)
SfiI 375 GGCCCCACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 77 CCCGGG CCC^GGG
SmlI 103 CTTAAG C^T[CT][GA]AG
StyI 179 CCAAGG C^C[AT][AT]GG
Tru9I 104 TTAA T^TAA
Van91I 213 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 77 CCCGGG C^CCGGG
XmaI 77 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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BanII 183 GGGCTC G[GA]GC[CT]^C
397 GGGCTC
BseRI 400 CTCCTC GAGGAG(10/8)
370 GAGGAG
BsrI 158 ACTGG ACTGG(1/-1)
381 ACTGG
DdeI 70 CTGAG C^T[ACGT]AG
259 CTCAG
DpnII 36 GATC ^GATC
252 GATC
DraII 73 AGGACCC [GA]G^G[ACGT]CC[CT]
374 AGGCCCC
DsaI 6 CCACGG C^C[GA][CT]GG
47 CCGTGG
EaeI 242 TGGCCA [CT]^GGCC[GA]
383 TGGCCG
EcoO109I 73 AGGACCC [GA]G^G[ACGT]CC[CT]
374 AGGCCCC
MboI 36 GATC ^GATC
252 GATC
NdeII 36 GATC ^GATC
252 GATC
PvuII 271 CAGCTG CAG^CTG
280 CAGCTG
Sau3AI 36 GATC ^GATC
252 GATC
TspRI 157 CACTG CA[GC]TG[ACGT][ACGT]^
380 CACTG
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
Bsp1286I BssKI Cac8I Cfr13I Fnu4HI
HaeIII HapII HpaII ItaI MnlI
MspA1I MspI MwoI NciI NlaIV
Sau96I ScrFI TseI