Restriction map for
pF1KE0404
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
Acc65I AccI AccII AclI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaI BseRI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspHI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeIII MamI MfeI MluI
MluNI MscI MunI MvnI NarI
NciI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tsp45I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 525 GACGTC GACGT^C
AccIII 9 TCCGGA T^CCGGA
AfaI 642 GTAC GT^AC
AflIII 54 ACATGT A^C[GA][CT]GT
AhdI 484 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 484 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 525 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 483 GGACC G^G[AT]CC
BanII 343 GAGCTC G[GA]GC[CT]^C
BbvCI 678 CCTCAGC CCTCAGC(-5/-2)
BlpI 46 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 46 GCTCAGC GC^T[ACGT]AGC
BseAI 9 TCCGGA T^CCGGA
BsmAI 693 GAGAC GTCTC(1/5)
BspEI 9 TCCGGA T^CCGGA
BspLU11I 54 ACATGT A^CATGT
BsrFI 571 GCCGGC [GA]^CCGG[CT]
CelII 46 GCTCAGC GC^T[ACGT]AGC
CfoI 15 GCGC GCG^C
Cfr10I 571 GCCGGC [GA]^CCGG[CT]
Eam1105I 484 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 805 GAAGAG CTCTTC(1/4)
HaeII 14 AGCGCC [GA]GCGC^[CT]
HgaI 595 GCGTC GACGC(5/10)
HhaI 15 GCGC GCG^C
HinP1I 15 GCGC G^CGC
HphI 62 TCACC GGTGA(8/7)
Ksp632I 805 GAAGAG CTCTTC(1/4)
MaeII 526 ACGT A^CGT
MroI 9 TCCGGA T^CCGGA
MseI 152 TTAA T^TAA
MslI 63 CACCTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 571 GCCGGC GCC^GGC
NcoI 386 CCATGG C^CATGG
NgoMIV 571 GCCGGC G^CCGGC
NlaIV 568 GGGGCC GG[ACGT]^[ACGT]CC
NspI 54 ACATGT [GA]CATG^[CT]
PflFI 525 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 609 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 6 GAGTC GAGTC(4/5)
PpuMI 482 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 642 GTAC GT^AC
SacI 343 GAGCTC GAGCT^C
SapI 805 GAAGAGC GCTCTTC(1/4)
SspI 146 AATATT AAT^ATT
TaqI 186 TCGA T^CGA
Tru9I 152 TTAA T^TAA
Tth111I 525 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 186 TCGA T^CGA
Van91I 609 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 190 GAATTC [GA]^AATT[CT]
451 GAATTC
AcyI 525 GACGTC G[GA]^CG[CT]C
594 GGCGTC
AlwI 432 GATCC GGATC(4/5)
777 GGATC
AlwNI 610 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
691 CAGAGACTG
ApoI 190 GAATTC [GA]^AATT[CT]
451 GAATTC
AspHI 343 GAGCTC G[AT]GC[AT]^C
370 GTGCTC
BfaI 19 CTAG C^TAG
94 CTAG
BmyI 343 GAGCTC G[AGT]GC[ACT]^C
370 GTGCTC
BsaHI 525 GACGTC G[GA]^CG[CT]C
594 GGCGTC
BsaWI 9 TCCGGA [AT]^CCGG[AT]
27 ACCGGA
BsiHKAI 343 GAGCTC G[AT]GC[AT]^C
370 GTGCTC
Bsp1286I 343 GAGCTC G[AGT]GC[ACT]^C
370 GTGCTC
BspMI 485 ACCTGC ACCTGC(4/8)
555 ACCTGC
BsrDI 683 GCAATG GCAATG(2/0)
796 GCAATG
BstF5I 75 GGATG GGATG(2/0)
801 GGATG
BstYI 431 AGATCC [GA]^GATC[CT]
777 GGATCT
DraII 482 AGGACCT [GA]G^G[ACGT]CC[CT]
674 AGGCCCT
DsaI 386 CCATGG C^C[GA][CT]GG
735 CCGTGG
EcoO109I 482 AGGACCT [GA]G^G[ACGT]CC[CT]
674 AGGCCCT
EcoRI 190 GAATTC G^AATTC
451 GAATTC
EcoT14I 386 CCATGG C^C[AT][AT]GG
560 CCTTGG
FauI 336 CCCGC CCCGC(4/6)
566 GCGGG
FokI 75 GGATG GGATG(9/13)
801 GGATG
Hin1I 525 GACGTC G[GA]^CG[CT]C
594 GGCGTC
MaeI 19 CTAG C^TAG
94 CTAG
MflI 431 AGATCC [GA]^GATC[CT]
777 GGATCT
PvuII 88 CAGCTG CAG^CTG
645 CAGCTG
StyI 386 CCATGG C^C[AT][AT]GG
560 CCTTGG
TfiI 710 GATTC G^A[AT]TC
740 GAATC
XhoII 431 AGATCC [GA]^GATC[CT]
777 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI BbvI BpmI BsaJI
BsiYI BslI BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MboI MboII MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp509I TspRI