Restriction map for
pF1KE0402
##################################
# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BanI BbeI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HphI KasI KpnI
KspI MaeIII MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 751 GTCTAC GT^[AC][GT]AC
AflIII 539 ACACGT A^C[GA][CT]GT
Alw44I 592 GTGCAC G^TGCAC
AlwNI 218 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 592 GTGCAC G^TGCAC
BamHI 764 GGATCC G^GATCC
BanII 170 GAGCCC G[GA]GC[CT]^C
BbrPI 540 CACGTG CAC^GTG
BbsI 54 GAAGAC GAAGAC(2/6)
BcnI 645 CCCGG CC^[GC]GG
BpmI 884 CTGGAG CTGGAG(16/14)
BpuAI 54 GAAGAC GAAGAC(2/6)
BsaAI 540 CACGTG [CT]AC^GT[GA]
BstYI 764 GGATCC [GA]^GATC[CT]
Cac8I 677 GCCTGC GC[ACGT]^[ACGT]GC
DraI 823 TTTAAA TTT^AAA
DrdI 742 GACATCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 683 CCACGG C^C[GA][CT]GG
EcoNI 175 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 384 CCTTGG C^C[AT][AT]GG
EcoT22I 341 ATGCAT ATGCA^T
HapII 646 CCGG C^CGG
HpaI 450 GTTAAC GTT^AAC
HpaII 646 CCGG C^CGG
MflI 764 GGATCC [GA]^GATC[CT]
MspA1I 323 CAGCTG C[AC]G^C[GT]G
MspI 646 CCGG C^CGG
MwoI 668 GCAAGGTAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 645 CCCGG CC^[GC]GG
NdeI 487 CATATG CA^TATG
NsiI 341 ATGCAT ATGCA^T
PleI 759 GAGTC GAGTC(4/5)
PmaCI 540 CACGTG CAC^GTG
PmlI 540 CACGTG CAC^GTG
PpuMI 353 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 197 CTGCAG CTGCA^G
PvuII 323 CAGCTG CAG^CTG
SfcI 197 CTGCAG C^T[GA][CT]AG
StyI 384 CCTTGG C^C[AT][AT]GG
TspRI 15 CAGTG CA[GC]TG[ACGT][ACGT]^
XbaI 738 TCTAGA T^CTAGA
XhoII 764 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 459 GAATTC [GA]^AATT[CT]
802 GAATTC
AlwI 765 GATCC GGATC(4/5)
764 GGATC
ApoI 459 GAATTC [GA]^AATT[CT]
802 GAATTC
Asp700I 297 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
846 GAAAGCATTC
AspHI 436 GTGCTC G[AT]GC[AT]^C
592 GTGCAC
BbvI 468 GCAGC GCAGC(8/12)
701 GCAGC
BfaI 505 CTAG C^TAG
739 CTAG
BsiHKAI 436 GTGCTC G[AT]GC[AT]^C
592 GTGCAC
BsmAI 887 GAGAC GTCTC(1/5)
895 GAGAC
BsmI 343 GCATTC GAATGC(1/-1)
850 GCATTC
DpnII 726 GATC ^GATC
765 GATC
DraII 184 GGGGCCT [GA]G^G[ACGT]CC[CT]
353 AGGTCCC
EcoO109I 184 GGGGCCT [GA]G^G[ACGT]CC[CT]
353 AGGTCCC
EcoRI 459 GAATTC G^AATTC
802 GAATTC
Fnu4HI 468 GCAGC GC^[ACGT]GC
701 GCAGC
HincII 66 GTTGAC GT[CT]^[GA]AC
450 GTTAAC
HindII 66 GTTGAC GT[CT]^[GA]AC
450 GTTAAC
ItaI 468 GCAGC GC^[ACGT]GC
701 GCAGC
MaeI 505 CTAG C^TAG
739 CTAG
MaeII 360 ACGT A^CGT
541 ACGT
MboI 726 GATC ^GATC
765 GATC
MslI 624 CACGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
717 CATTGACATG
NdeII 726 GATC ^GATC
765 GATC
SapI 160 GAAGAGC GCTCTTC(1/4)
655 GAAGAGC
Sau3AI 726 GATC ^GATC
765 GATC
TseI 468 GCAGC G^C[AT]GC
701 GCAGC
XmnI 297 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
846 GAAAGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI AvaII BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
EarI EcoRII FokI HaeIII HinfI
Ksp632I MboII MnlI MseI MvaI
NlaIII NlaIV RsaI Sau96I ScrFI
SfaNI TfiI Tru9I Tsp509I