Restriction map for
pF1KB6717
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AluI Alw44I AlwNI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BanI BanII BbeI BbrPI BbsI
BbvCI BbvI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Cac8I CelII CfoI ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MboII
MfeI MluI MseI MslI MunI
MvnI MwoI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tru9I TseI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 20 TCCGGA T^CCGGA
AocI 81 CCTGAGG CC^T[ACGT]AGG
AvaII 112 GGACC G^G[AT]CC
BalI 5 TGGCCA TGG^CCA
BamHI 17 GGATCC G^GATCC
BpmI 182 CTGGAG CTGGAG(16/14)
BsaJI 115 CCATGG C^C[ACGT][ACGT]GG
BsaWI 20 TCCGGA [AT]^CCGG[AT]
BseAI 20 TCCGGA T^CCGGA
BseRI 94 GAGGAG GAGGAG(10/8)
BsmAI 185 GAGAC GTCTC(1/5)
BsmFI 111 GGGAC GGGAC(10/14)
BspEI 20 TCCGGA T^CCGGA
BsrFI 244 ACCGGC [GA]^CCGG[CT]
BssKI 181 CCTGG ^CC[ACGT]GG
BstNI 181 CCTGG CC^[AT]GG
BstYI 17 GGATCC [GA]^GATC[CT]
Bsu36I 81 CCTGAGG CC^T[ACGT]AGG
Cfr10I 244 ACCGGC [GA]^CCGG[CT]
Cfr13I 112 GGACC G^G[ACGT]CC
DpnII 18 GATC ^GATC
DsaI 115 CCATGG C^C[GA][CT]GG
EaeI 5 TGGCCA [CT]^GGCC[GA]
Eco81I 81 CCTGAGG CC^T[ACGT]AGG
EcoRII 181 CCTGG ^CC[AT]GG
EcoT14I 115 CCATGG C^C[AT][AT]GG
Fnu4HI 87 GCCGC GC^[ACGT]GC
HincII 158 GTCAAC GT[CT]^[GA]AC
HindII 158 GTCAAC GT[CT]^[GA]AC
HinfI 225 GAGTC G^A[ACGT]TC
ItaI 87 GCCGC GC^[ACGT]GC
MaeII 68 ACGT A^CGT
MboI 18 GATC ^GATC
MflI 17 GGATCC [GA]^GATC[CT]
MluNI 5 TGGCCA TGG^CCA
MroI 20 TCCGGA T^CCGGA
MscI 5 TGGCCA TGG^CCA
MspA1I 13 CAGCGG C[AC]G^C[GT]G
MvaI 181 CCTGG CC^[AT]GG
NcoI 115 CCATGG C^CATGG
NdeII 18 GATC ^GATC
NlaIII 116 CATG CATG^
PleI 225 GAGTC GAGTC(4/5)
Sau3AI 18 GATC ^GATC
Sau96I 112 GGACC G^G[ACGT]CC
ScrFI 181 CCTGG CC^[ACGT]GG
StyI 115 CCATGG C^C[AT][AT]GG
Tsp45I 227 GTCAC ^GT[GC]AC
Tsp509I 170 AATT ^AATT
TspRI 128 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 17 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 11 enzymes) #
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AciI 88 CCGC CCGC(-3/-1)
15 GCGG
AlwI 18 GATCC GGATC(4/5)
17 GGATC
BstF5I 215 CATCC GGATG(2/0)
23 GGATG
DdeI 82 CTGAG C^T[ACGT]AG
153 CTCAG
FokI 215 CATCC GGATG(9/13)
23 GGATG
HapII 21 CCGG C^CGG
245 CCGG
HpaII 21 CCGG C^CGG
245 CCGG
HphI 53 TCACC GGTGA(8/7)
228 TCACC
MaeIII 211 GTAAC ^GT[ACGT]AC
227 GTCAC
MspI 21 CCGG C^CGG
245 CCGG
NlaIV 17 GGATCC GG[ACGT]^[ACGT]CC
111 GGGACC
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# 3 OR MORE CUT ENZYMES ( 4 enzymes) #
#########################################
BsiYI BslI HaeIII MnlI