##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       AccII       AccIII      AciI        AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp700I     AspEI       AspHI       AspI        AvaII       
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
Bsp1286I    BspEI       BspMI       BsrBI       BsrFI       
BssHII      BssKI       BssSI       Bst1107I    BstBI       
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      Cfr13I      CpoI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HapII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KspI        MaeI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        MvaI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PstI        PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SwaI        Tsp45I      Tth111I     Van91I      XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I        849  GGTACC               G^GTACC
AccI          448  GTCTAC               GT^[AC][GT]AC
AcsI          886  AAATTT               [GA]^AATT[CT]
AflIII        695  ACATGT               A^C[GA][CT]GT
AluI          303  AGCT                 AG^CT
AlwI          328  GATCC                GGATC(4/5)
AlwNI         765  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          886  AAATTT               [GA]^AATT[CT]
AseI          498  ATTAAT               AT^TAAT
AsnI          498  ATTAAT               AT^TAAT
Asp718I       849  GGTACC               G^GTACC
AvaI          237  CTCGGG               C^[CT]CG[GA]G
BanI          849  GGTACC               G^G[CT][GA]CC
BbsI           45  GAAGAC               GAAGAC(2/6)
BciVI         905  GGATAC               GTATCC(6/5)
BpuAI          45  GAAGAC               GAAGAC(2/6)
BsaJI          27  CCTTGG               C^C[ACGT][ACGT]GG
BsgI          581  CTGCAC               GTGCAG(16/14)
BsoBI         237  CTCGGG               C^[CT]CG[GA]G
Bsp1407I      692  TGTACA               T^GTACA
BspDI         560  ATCGAT               AT^CGAT
BspHI         438  TCATGA               T^CATGA
BspLU11I      695  ACATGT               A^CATGT
BsrDI         193  GCAATG               GCAATG(2/0)
BsrGI         692  TGTACA               T^GTACA
BstAPI        825  GCAAGATTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I          22  GCATGC               GC[ACGT]^[ACGT]GC
CfoI          257  GCGC                 GCG^C
ClaI          560  ATCGAT               AT^CGAT
DdeI          912  CTCAG                C^T[ACGT]AG
EcoT14I        27  CCTTGG               C^C[AT][AT]GG
HaeIII        631  GGCC                 GG^CC
HhaI          257  GCGC                 GCG^C
HinP1I        257  GCGC                 G^CGC
HinfI         151  GAATC                G^A[ACGT]TC
KpnI          849  GGTACC               GGTAC^C
MaeII         416  ACGT                 A^CGT
MslI          781  CATGCCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI          825  GCAAGATTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PshBI         498  ATTAAT               AT^TAAT
RcaI          438  TCATGA               T^CATGA
SmlI          820  CTTGAG               C^T[CT][GA]AG
SphI           22  GCATGC               GCATG^C
SspBI         692  TGTACA               T^GTACA
SspI          131  AATATT               AAT^ATT
StuI          630  AGGCCT               AGG^CCT
StyI           27  CCTTGG               C^C[AT][AT]GG
TfiI          151  GAATC                G^A[AT]TC
XcmI          800  CCATTTCCAGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

DraI           58  TTTAAA               TTT^AAA
              565  TTTAAA              
EarI          518  CTCTTC               CTCTTC(1/4)
              760  CTCTTC              
HphI          525  GGTGA                GGTGA(8/7)
              311  TCACC               
Ksp632I       518  CTCTTC               CTCTTC(1/4)
              760  CTCTTC              
NlaIV         849  GGTACC               GG[ACGT]^[ACGT]CC
              919  GGAGCC              
Tsp509I       600  AATT                 ^AATT
              887  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AfaI        BbvI        BsmI        BsrI        DpnII       
Fnu4HI      ItaI        MaeIII      MboI        MboII       
MnlI        MseI        NdeII       NlaIII      NspI        
RsaI        Sau3AI      SfaNI       TaqI        Tru9I       
TseI        TspRI       TthHB8I