Restriction map for
pF1KE0007
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsoBI BspDI BspEI
BspLU11I BsrDI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraIII
DrdI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MflI MluI MroI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AhdI 809 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 809 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 1474 GGCACC G^G[CT][GA]CC
BanII 484 GAGCTC G[GA]GC[CT]^C
BbsI 679 GTCTTC GAAGAC(2/6)
BciVI 25 GTATCC GTATCC(6/5)
BpmI 487 CTCCAG CTGGAG(16/14)
BpuAI 679 GTCTTC GAAGAC(2/6)
BseRI 481 GAGGAG GAGGAG(10/8)
BspHI 1043 TCATGA T^CATGA
BsrBI 1328 CCGCTC CCGCTC(-3/-3)
BsrFI 1471 ACCGGC [GA]^CCGG[CT]
BssSI 198 CACGAG CACGAG(-5/-1)
BstAPI 996 GCATGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 806 CCAGACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1188 GCGC GCG^C
Cfr10I 1471 ACCGGC [GA]^CCGG[CT]
DraI 531 TTTAAA TTT^AAA
Eam1105I 809 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 781 GATATC GAT^ATC
HapII 1472 CCGG C^CGG
HhaI 1188 GCGC GCG^C
HinP1I 1188 GCGC G^CGC
HindIII 139 AAGCTT A^AGCTT
HpaII 1472 CCGG C^CGG
MaeII 519 ACGT A^CGT
MspI 1472 CCGG C^CGG
NspI 1373 ACATGC [GA]CATG^[CT]
PleI 1158 GAGTC GAGTC(4/5)
PvuII 1432 CAGCTG CAG^CTG
RcaI 1043 TCATGA T^CATGA
SacI 484 GAGCTC GAGCT^C
TfiI 499 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwI 721 GATCC GGATC(4/5)
1217 GGATC
BsmBI 520 CGTCTC CGTCTC(1/5)
1405 GAGACG
BsmFI 190 GTCCC GGGAC(10/14)
791 GTCCC
BspMI 256 ACCTGC ACCTGC(4/8)
1352 ACCTGC
DpnII 721 GATC ^GATC
1218 GATC
DraII 984 AGGTCCT [GA]G^G[ACGT]CC[CT]
1107 GGGTCCT
EcoO109I 984 AGGTCCT [GA]G^G[ACGT]CC[CT]
1107 GGGTCCT
HinfI 499 GATTC G^A[ACGT]TC
1158 GAGTC
MboI 721 GATC ^GATC
1218 GATC
MslI 462 CATGTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1414 CACCCTGATG
MspA1I 1227 CAGCGG C[AC]G^C[GT]G
1432 CAGCTG
NdeII 721 GATC ^GATC
1218 GATC
PpuMI 984 AGGTCCT [GA]G^G[AT]CC[CT]
1107 GGGTCCT
PshAI 44 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
346 GACGGCTGTC
PstI 1171 CTGCAG CTGCA^G
1234 CTGCAG
Sau3AI 721 GATC ^GATC
1218 GATC
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
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AciI AcsI AfaI AluI ApoI
AspHI AvaII BalI BbvI BfaI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DsaI EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HphI
ItaI MaeI MaeIII MboII MluNI
MnlI MscI MseI MvaI MwoI
NcoI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI