Restriction map for
pF1KE0111
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp718I AviII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BsaAI BsaBI BsaI BseAI BsiWI
BsmAI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BsrI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EarI EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I XbaI
XcmI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AhdI 48 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 338 AGCGCT AGC^GCT
Asp700I 336 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 48 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 703 GAGCAC G[AT]GC[AT]^C
AspI 474 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 255 CCTAGG C^CTAGG
BbeI 92 GGCGCC GGCGC^C
BbsI 1018 GTCTTC GAAGAC(2/6)
BglI 209 GCCAGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 998 AGATCT A^GATCT
BlnI 255 CCTAGG C^CTAGG
BlpI 955 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 955 GCTGAGC GC^T[ACGT]AGC
BpuAI 1018 GTCTTC GAAGAC(2/6)
BsaWI 965 ACCGGA [AT]^CCGG[AT]
BseRI 985 GAGGAG GAGGAG(10/8)
BsgI 976 GTGCAG GTGCAG(16/14)
BsiHKAI 703 GAGCAC G[AT]GC[AT]^C
BsmI 645 GCATTC GAATGC(1/-1)
BsrBI 155 CCGCTC CCGCTC(-3/-3)
BssSI 617 CACGAG CACGAG(-5/-1)
BstAPI 616 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 33 CATCC GGATG(2/0)
BstXI 450 CCAGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 955 GCTGAGC GC^T[ACGT]AGC
DsaI 162 CCGTGG C^C[GA][CT]GG
EagI 73 CGGCCG C^GGCCG
Eam1105I 48 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 73 CGGCCG C^GGCCG
Eco47III 338 AGCGCT AGC^GCT
Eco52I 73 CGGCCG C^GGCCG
EcoT14I 255 CCTAGG C^C[AT][AT]GG
FokI 33 CATCC GGATG(9/13)
HphI 406 TCACC GGTGA(8/7)
KasI 92 GGCGCC G^GCGCC
MaeII 227 ACGT A^CGT
MaeIII 608 GTCAC ^GT[ACGT]AC
NarI 92 GGCGCC GG^CGCC
NspI 833 GCATGC [GA]CATG^[CT]
PflFI 474 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 846 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 658 CTGCAG CTGCA^G
PvuII 889 CAGCTG CAG^CTG
SexAI 561 ACCAGGT A^CC[AT]GGT
SfoI 92 GGCGCC GGC^GCC
SmaI 431 CCCGGG CCC^GGG
SphI 833 GCATGC GCATG^C
StuI 585 AGGCCT AGG^CCT
StyI 255 CCTAGG C^C[AT][AT]GG
TfiI 330 GATTC G^A[AT]TC
Tsp45I 608 GTCAC ^GT[GC]AC
Tth111I 474 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 846 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 431 CCCGGG C^CCGGG
XmaI 431 CCCGGG C^CCGGG
XmnI 336 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcyI 92 GGCGCC G[GA]^CG[CT]C
217 GGCGTC
AfaI 414 GTAC GT^AC
907 GTAC
AocI 289 CCTAAGG CC^T[ACGT]AGG
312 CCTGAGG
BanI 92 GGCGCC G^G[CT][GA]CC
184 GGTGCC
BsaHI 92 GGCGCC G[GA]^CG[CT]C
217 GGCGTC
BsiEI 73 CGGCCG CG[GA][CT]^CG
135 CGACCG
BspMI 660 GCAGGT ACCTGC(4/8)
978 GCAGGT
Bsu36I 289 CCTAAGG CC^T[ACGT]AGG
312 CCTGAGG
EaeI 73 CGGCCG [CT]^GGCC[GA]
207 CGGCCA
Eco81I 289 CCTAAGG CC^T[ACGT]AGG
312 CCTGAGG
HgaI 48 GACGC GACGC(5/10)
218 GCGTC
Hin1I 92 GGCGCC G[GA]^CG[CT]C
217 GGCGTC
MboII 1026 GAAGA GAAGA(8/7)
1019 TCTTC
MslI 706 CACTACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
828 CACGCGCATG
PaeR7I 97 CTCGAG C^TCGAG
241 CTCGAG
RsaI 414 GTAC GT^AC
907 GTAC
SfaNI 20 GCATC GCATC(5/9)
173 GCATC
SfcI 66 CTACAG C^T[GA][CT]AG
658 CTGCAG
TaqI 98 TCGA T^CGA
242 TCGA
TthHB8I 98 TCGA T^CGA
242 TCGA
XhoI 97 CTCGAG C^TCGAG
241 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
ApaI AvaI AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BssKI BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeI MboI MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI Sau3AI Sau96I ScrFI
SmlI TseI TspRI XhoII