Restriction map for
pF1KE0476
##################################
# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BbeI
BbrPI BcgI BciVI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
EcoT22I FseI FspI HaeII HhaI
HinP1I HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MroI
MseI MslI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I TthHB8I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 505 GTCTAC GT^[AC][GT]AC
AcsI 349 AAATTC [GA]^AATT[CT]
AcyI 556 GGCGTC G[GA]^CG[CT]C
AocI 69 CCTCAGG CC^T[ACGT]AGG
ApoI 349 AAATTC [GA]^AATT[CT]
AspHI 459 GAGCAC G[AT]GC[AT]^C
AspI 700 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 579 TGGCCA TGG^CCA
BbsI 706 GTCTTC GAAGAC(2/6)
BbvCI 624 GCTGAGG CCTCAGC(-5/-2)
BclI 410 TGATCA T^GATCA
BglI 299 GCCCCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 314 CTGGAG CTGGAG(16/14)
BpuAI 706 GTCTTC GAAGAC(2/6)
BsaHI 556 GGCGTC G[GA]^CG[CT]C
BsgI 395 CTGCAC GTGCAG(16/14)
BsiHKAI 459 GAGCAC G[AT]GC[AT]^C
BssSI 456 CACGAG CACGAG(-5/-1)
BstEII 567 GGTCACC G^GT[ACGT]ACC
BstPI 567 GGTCACC G^GT[ACGT]ACC
Bsu36I 69 CCTCAGG CC^T[ACGT]AGG
EarI 724 CTCTTC CTCTTC(1/4)
Eco81I 69 CCTCAGG CC^T[ACGT]AGG
EcoO65I 567 GGTCACC G^GT[ACGT]ACC
FauI 438 CCCGC CCCGC(4/6)
FbaI 410 TGATCA T^GATCA
Hin1I 556 GGCGTC G[GA]^CG[CT]C
HincII 604 GTCAAC GT[CT]^[GA]AC
HindII 604 GTCAAC GT[CT]^[GA]AC
Ksp632I 724 CTCTTC CTCTTC(1/4)
MluI 512 ACGCGT A^CGCGT
MluNI 579 TGGCCA TGG^CCA
MscI 579 TGGCCA TGG^CCA
MspA1I 552 CAGCGG C[AC]G^C[GT]G
PflFI 700 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 723 GCTCTTC GCTCTTC(1/4)
SmaI 196 CCCGGG CCC^GGG
Tsp509I 350 AATT ^AATT
Tth111I 700 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 196 CCCGGG C^CCGGG
XmaI 196 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 423 CGCG CG^CG
513 CGCG
AfaI 147 GTAC GT^AC
546 GTAC
AflIII 512 ACGCGT A^C[GA][CT]GT
584 ACACGT
AluI 16 AGCT AG^CT
41 AGCT
ApaI 10 GGGCCC GGGCC^C
163 GGGCCC
AvaI 196 CCCGGG C^[CT]CG[GA]G
609 CTCGGG
BamHI 101 GGATCC G^GATCC
200 GGATCC
BanI 129 GGCACC G^G[CT][GA]CC
291 GGTGCC
BseRI 328 GAGGAG GAGGAG(10/8)
643 GAGGAG
BsmFI 270 GGGAC GGGAC(10/14)
484 GTCCC
BsoBI 196 CCCGGG C^[CT]CG[GA]G
609 CTCGGG
BspMI 475 ACCTGC ACCTGC(4/8)
633 GCAGGT
BsrI 463 ACTGG ACTGG(1/-1)
354 CCAGT
BstUI 423 CGCG CG^CG
513 CGCG
BstYI 101 GGATCC [GA]^GATC[CT]
200 GGATCC
DdeI 70 CTCAG C^T[ACGT]AG
625 CTGAG
DsaI 266 CCATGG C^C[GA][CT]GG
302 CCATGG
EaeI 243 CGGCCA [CT]^GGCC[GA]
579 TGGCCA
HinfI 182 GAATC G^A[ACGT]TC
416 GATTC
MaeII 5 ACGT A^CGT
586 ACGT
MaeIII 370 GTCAC ^GT[ACGT]AC
568 GTCAC
MboII 707 TCTTC GAAGA(8/7)
725 TCTTC
MflI 101 GGATCC [GA]^GATC[CT]
200 GGATCC
MvnI 423 CGCG CG^CG
513 CGCG
NcoI 266 CCATGG C^CATGG
302 CCATGG
PpuMI 207 AGGACCC [GA]G^G[AT]CC[CT]
270 GGGACCC
RsaI 147 GTAC GT^AC
546 GTAC
TfiI 182 GAATC G^A[AT]TC
416 GATTC
Tsp45I 370 GTCAC ^GT[GC]AC
568 GTCAC
TspRI 359 CAGTG CA[GC]TG[ACGT][ACGT]^
462 CACTG
XhoII 101 GGATCC [GA]^GATC[CT]
200 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AlwI AvaII BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DpnII DraII EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HpaII HphI ItaI MboI
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI StyI TseI