Restriction map for
pF1KE0304
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AviII BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BglI BpuAI BsaAI BsaHI BseAI
BsgI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstXI
Bsu36I Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I FauI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MfeI
MluNI MroI MscI MunI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 451 GTATAC GT^[AC][GT]AC
AfaI 1310 GTAC GT^AC
AflIII 98 ACGCGT A^C[GA][CT]GT
AvrII 1225 CCTAGG C^CTAGG
BanI 1421 GGTGCC G^G[CT][GA]CC
BclI 815 TGATCA T^GATCA
BglII 846 AGATCT A^GATCT
BlnI 1225 CCTAGG C^CTAGG
BlpI 595 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 595 GCTGAGC GC^T[ACGT]AGC
BsaBI 478 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 126 ACCGGA [AT]^CCGG[AT]
BseRI 37 GAGGAG GAGGAG(10/8)
BsmBI 271 CGTCTC CGTCTC(1/5)
BsmFI 81 GTCCC GGGAC(10/14)
BspHI 579 TCATGA T^CATGA
BsrDI 506 GCAATG GCAATG(2/0)
Bst1107I 451 GTATAC GTA^TAC
BstZ17I 451 GTATAC GTA^TAC
CelII 595 GCTGAGC GC^T[ACGT]AGC
DraI 852 TTTAAA TTT^AAA
EaeI 701 TGGCCG [CT]^GGCC[GA]
EarI 1092 CTCTTC CTCTTC(1/4)
EcoRI 1326 GAATTC G^AATTC
EcoRV 1128 GATATC GAT^ATC
EcoT22I 412 ATGCAT ATGCA^T
FbaI 815 TGATCA T^GATCA
HaeII 500 GGCGCT [GA]GCGC^[CT]
HapII 127 CCGG C^CGG
HpaII 127 CCGG C^CGG
Ksp632I 1092 CTCTTC CTCTTC(1/4)
MamI 478 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 98 ACGCGT A^CGCGT
MslI 970 CACCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 178 CAGCTG C[AC]G^C[GT]G
MspI 127 CCGG C^CGG
NdeI 409 CATATG CA^TATG
NsiI 412 ATGCAT ATGCA^T
PvuII 178 CAGCTG CAG^CTG
RcaI 579 TCATGA T^CATGA
RsaI 1310 GTAC GT^AC
TaqI 191 TCGA T^CGA
TfiI 1159 GAATC G^A[AT]TC
TthHB8I 191 TCGA T^CGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 64 CGCG CG^CG
99 CGCG
AlwNI 136 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
1163 CAGCATCTG
AseI 832 ATTAAT AT^TAAT
1402 ATTAAT
AsnI 832 ATTAAT AT^TAAT
1402 ATTAAT
AspHI 598 GAGCAC G[AT]GC[AT]^C
1408 GAGCAC
AvaII 117 GGACC G^G[AT]CC
391 GGTCC
BbvI 236 GCAGC GCAGC(8/12)
503 GCTGC
BmyI 598 GAGCAC G[AGT]GC[ACT]^C
1408 GAGCAC
BsaI 319 GAGACC GGTCTC(1/5)
945 GGTCTC
BsiHKAI 598 GAGCAC G[AT]GC[AT]^C
1408 GAGCAC
Bsp1286I 598 GAGCAC G[AGT]GC[ACT]^C
1408 GAGCAC
BstUI 64 CGCG CG^CG
99 CGCG
CfoI 63 GCGC GCG^C
501 GCGC
EcoT14I 1048 CCAAGG C^C[AT][AT]GG
1225 CCTAGG
HgaI 97 GACGC GACGC(5/10)
100 GCGTC
HhaI 63 GCGC GCG^C
501 GCGC
HinP1I 63 GCGC G^CGC
501 GCGC
MvnI 64 CGCG CG^CG
99 CGCG
MwoI 419 GCATCAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
513 GCCGTGCCAGC
NspI 342 ACATGC [GA]CATG^[CT]
825 ACATGC
PleI 79 GAGTC GAGTC(4/5)
1393 GAGTC
PshBI 832 ATTAAT AT^TAAT
1402 ATTAAT
StyI 1048 CCAAGG C^C[AT][AT]GG
1225 CCTAGG
TseI 236 GCAGC G^C[AT]GC
503 GCTGC
XcmI 603 CCAGAAAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1182 CCAGGATGTGAGTGG
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
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AciI AcsI AluI AlwI ApoI
Asp700I BfaI BpmI BsaJI BsiYI
BslI BsmAI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MvaI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI Tru9I Tsp45I
Tsp509I TspRI XhoII XmnI